Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system
ABSTRACTBacteria have developed various defense mechanisms against phages. Abortive infection (Abi), a bacterial defense mechanism, can be achieved through various means, including toxin-antitoxin systems, cyclic oligonucleotide-based antiphage signaling systems, and retrons. AbpA and AbpB (AbpAB) d...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2023-12-01
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| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/msphere.00372-23 |
| _version_ | 1797384683437162496 |
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| author | Takaomi Sasaki Saya Takita Takashi Fujishiro Yunosuke Shintani Satoki Nojiri Ryota Yasui Tetsuro Yonesaki Yuichi Otsuka |
| author_facet | Takaomi Sasaki Saya Takita Takashi Fujishiro Yunosuke Shintani Satoki Nojiri Ryota Yasui Tetsuro Yonesaki Yuichi Otsuka |
| author_sort | Takaomi Sasaki |
| collection | DOAJ |
| description | ABSTRACTBacteria have developed various defense mechanisms against phages. Abortive infection (Abi), a bacterial defense mechanism, can be achieved through various means, including toxin-antitoxin systems, cyclic oligonucleotide-based antiphage signaling systems, and retrons. AbpA and AbpB (AbpAB) defend against many lytic phages harboring double-stranded DNA genomes in Escherichia coli; however, how AbpAB senses phage infection and inhibits its propagation remains unclear. Here, we demonstrated that AbpAB inhibited the growth of the φX174 lytic phage with single-stranded DNA (ssDNA) as well as the lysogenization and induction of the Sakai prophage 5 lysogenic phage. The AbpAB defense system limits T4 and φX174 phage propagation via Abi. AbpA contains a nuclease domain at its N-terminus, and AbpB has an ATP-dependent RNA helicase domain; both domains are required for phage defense. This system is activated by phage Gp32 binding to ssDNA and inhibits E. coli growth. Without phage infection, DNA replication inhibitors or defects in the DNA repair factors RecB and RecC activate this system. Therefore, the E. coli AbpAB defense system may sense DNA-protein complexes, including the phage-encoded ssDNA-binding protein or those formed by interrupting host DNA replication or repair.IMPORTANCEAlthough numerous phage defense systems have recently been discovered in bacteria, how these systems defend against phage propagation or sense phage infections remains unclear. The Escherichia coli AbpAB defense system targets several lytic and lysogenic phages harboring DNA genomes. A phage-encoded single-stranded DNA-binding protein, Gp32, activates this system similar to other phage defense systems such as Retron-Eco8, Hachiman, ShosTA, Nhi, and Hna. DNA replication inhibitors or defects in DNA repair factors activate the AbpAB system, even without phage infection. This is one of the few examples of activating phage defense systems without phage infection or proteins. The AbpAB defense system may be activated by sensing specific DNA-protein complexes. |
| first_indexed | 2024-03-08T21:40:13Z |
| format | Article |
| id | doaj.art-84a387e9d8c349f28cdfd39c8348892b |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-03-08T21:40:13Z |
| publishDate | 2023-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-84a387e9d8c349f28cdfd39c8348892b2023-12-20T14:01:03ZengAmerican Society for MicrobiologymSphere2379-50422023-12-018610.1128/msphere.00372-23Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense systemTakaomi Sasaki0Saya Takita1Takashi Fujishiro2Yunosuke Shintani3Satoki Nojiri4Ryota Yasui5Tetsuro Yonesaki6Yuichi Otsuka7Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University, Saitama, JapanDepartment of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University, Saitama, JapanDepartment of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University, Saitama, JapanDepartment of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University, Saitama, JapanDepartment of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University, Saitama, JapanDepartment of Biological Sciences, Graduate School of Science, Osaka University, Osaka, JapanDepartment of Biological Sciences, Graduate School of Science, Osaka University, Osaka, JapanDepartment of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University, Saitama, JapanABSTRACTBacteria have developed various defense mechanisms against phages. Abortive infection (Abi), a bacterial defense mechanism, can be achieved through various means, including toxin-antitoxin systems, cyclic oligonucleotide-based antiphage signaling systems, and retrons. AbpA and AbpB (AbpAB) defend against many lytic phages harboring double-stranded DNA genomes in Escherichia coli; however, how AbpAB senses phage infection and inhibits its propagation remains unclear. Here, we demonstrated that AbpAB inhibited the growth of the φX174 lytic phage with single-stranded DNA (ssDNA) as well as the lysogenization and induction of the Sakai prophage 5 lysogenic phage. The AbpAB defense system limits T4 and φX174 phage propagation via Abi. AbpA contains a nuclease domain at its N-terminus, and AbpB has an ATP-dependent RNA helicase domain; both domains are required for phage defense. This system is activated by phage Gp32 binding to ssDNA and inhibits E. coli growth. Without phage infection, DNA replication inhibitors or defects in the DNA repair factors RecB and RecC activate this system. Therefore, the E. coli AbpAB defense system may sense DNA-protein complexes, including the phage-encoded ssDNA-binding protein or those formed by interrupting host DNA replication or repair.IMPORTANCEAlthough numerous phage defense systems have recently been discovered in bacteria, how these systems defend against phage propagation or sense phage infections remains unclear. The Escherichia coli AbpAB defense system targets several lytic and lysogenic phages harboring DNA genomes. A phage-encoded single-stranded DNA-binding protein, Gp32, activates this system similar to other phage defense systems such as Retron-Eco8, Hachiman, ShosTA, Nhi, and Hna. DNA replication inhibitors or defects in DNA repair factors activate the AbpAB system, even without phage infection. This is one of the few examples of activating phage defense systems without phage infection or proteins. The AbpAB defense system may be activated by sensing specific DNA-protein complexes.https://journals.asm.org/doi/10.1128/msphere.00372-23bacteriophagesEscherichia coliphage defense systemAbpA-AbpB |
| spellingShingle | Takaomi Sasaki Saya Takita Takashi Fujishiro Yunosuke Shintani Satoki Nojiri Ryota Yasui Tetsuro Yonesaki Yuichi Otsuka Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system mSphere bacteriophages Escherichia coli phage defense system AbpA-AbpB |
| title | Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system |
| title_full | Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system |
| title_fullStr | Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system |
| title_full_unstemmed | Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system |
| title_short | Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system |
| title_sort | phage single stranded dna binding protein or host dna damage triggers the activation of the abpab phage defense system |
| topic | bacteriophages Escherichia coli phage defense system AbpA-AbpB |
| url | https://journals.asm.org/doi/10.1128/msphere.00372-23 |
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