Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids
Summary: Bile ducts are essential for bile transport and consist of complex branching tubular networks. Human patient-derived cholangiocyte develops a cystic rather than branching duct morphology. Here, we present a protocol to establish branching morphogenesis in cholangiocyte and cholangiocarcinom...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723003982 |
| _version_ | 1827904489568337920 |
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| author | Kimberley Ober Floris J.M. Roos Gilles S. van Tienderen Kübra Köten Annelot Klaassen Wunan Mi Luc J.W. van der Laan Monique M.A. Verstegen |
| author_facet | Kimberley Ober Floris J.M. Roos Gilles S. van Tienderen Kübra Köten Annelot Klaassen Wunan Mi Luc J.W. van der Laan Monique M.A. Verstegen |
| author_sort | Kimberley Ober |
| collection | DOAJ |
| description | Summary: Bile ducts are essential for bile transport and consist of complex branching tubular networks. Human patient-derived cholangiocyte develops a cystic rather than branching duct morphology. Here, we present a protocol to establish branching morphogenesis in cholangiocyte and cholangiocarcinoma organoids. We describe steps for the initiation, maintenance, and expansion of intrahepatic cholangiocyte organoids branching morphology. This protocol enables the study of organ-specific and mesenchymal-independent branching morphogenesis and provides an improved model to study biliary function and diseases.For complete details on the use and execution of this protocol, please refer to Roos et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-13T00:28:10Z |
| format | Article |
| id | doaj.art-84a4580cad5643e5aaecc2b8f27e89c6 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-13T00:28:10Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-84a4580cad5643e5aaecc2b8f27e89c62023-07-11T04:06:42ZengElsevierSTAR Protocols2666-16672023-09-0143102431Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoidsKimberley Ober0Floris J.M. Roos1Gilles S. van Tienderen2Kübra Köten3Annelot Klaassen4Wunan Mi5Luc J.W. van der Laan6Monique M.A. Verstegen7Department of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands; Corresponding authorWellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge and NIHR Biomedical Research Centre, Cambridge, UK; Department of Surgery, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UKDepartment of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the NetherlandsDepartment of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the NetherlandsDepartment of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the NetherlandsDepartment of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the NetherlandsDepartment of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the NetherlandsDepartment of Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, the Netherlands; Corresponding authorSummary: Bile ducts are essential for bile transport and consist of complex branching tubular networks. Human patient-derived cholangiocyte develops a cystic rather than branching duct morphology. Here, we present a protocol to establish branching morphogenesis in cholangiocyte and cholangiocarcinoma organoids. We describe steps for the initiation, maintenance, and expansion of intrahepatic cholangiocyte organoids branching morphology. This protocol enables the study of organ-specific and mesenchymal-independent branching morphogenesis and provides an improved model to study biliary function and diseases.For complete details on the use and execution of this protocol, please refer to Roos et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723003982CancerCell BiologyDevelopmental biologyOrganoids |
| spellingShingle | Kimberley Ober Floris J.M. Roos Gilles S. van Tienderen Kübra Köten Annelot Klaassen Wunan Mi Luc J.W. van der Laan Monique M.A. Verstegen Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids STAR Protocols Cancer Cell Biology Developmental biology Organoids |
| title | Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids |
| title_full | Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids |
| title_fullStr | Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids |
| title_full_unstemmed | Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids |
| title_short | Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids |
| title_sort | protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids |
| topic | Cancer Cell Biology Developmental biology Organoids |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723003982 |
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