Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid production
Abstract Background Oleaginous yeasts are a promising candidate for the sustainable conversion of lignocellulosic feedstocks into fuels and chemicals, but their growth on these substrates can be inhibited as a result of upstream pretreatment and enzymatic hydrolysis conditions. Previous studies indi...
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
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| Online Access: | https://doi.org/10.1186/s13068-023-02429-6 |
| _version_ | 1827604107656953856 |
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| author | William Woodruff Narendra Naik Deshavath Vionna Susanto Christopher V. Rao Vijay Singh |
| author_facet | William Woodruff Narendra Naik Deshavath Vionna Susanto Christopher V. Rao Vijay Singh |
| author_sort | William Woodruff |
| collection | DOAJ |
| description | Abstract Background Oleaginous yeasts are a promising candidate for the sustainable conversion of lignocellulosic feedstocks into fuels and chemicals, but their growth on these substrates can be inhibited as a result of upstream pretreatment and enzymatic hydrolysis conditions. Previous studies indicate a high citrate buffer concentration during hydrolysis inhibits downstream cell growth and ethanol fermentation in Saccharomyces cerevisiae. In this study, an engineered Rhodosporidium toruloides strain with enhanced lipid accumulation was grown on sorghum hydrolysate with high and low citrate buffer concentrations. Results Both hydrolysis conditions resulted in similar sugar recovery rates and concentrations. No significant differences in cell growth, sugar utilization rates, or lipid production rates were observed between the two citrate buffer conditions during batch fermentation of R. toruloides. Under fed-batch growth on low-citrate hydrolysate a lipid titer of 16.7 g/L was obtained. Conclusions Citrate buffer was not found to inhibit growth or lipid production in this engineered R. toruloides strain, nor did reducing the citrate buffer concentration negatively affect sugar yields in the hydrolysate. As this process is scaled-up, $131 per ton of hydrothermally pretreated biomass can be saved by use of the lower citrate buffer concentration during enzymatic hydrolysis. Graphical Abstract |
| first_indexed | 2024-03-09T05:54:24Z |
| format | Article |
| id | doaj.art-84a55167ef87438b8cc792b121340df3 |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-03-09T05:54:24Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-84a55167ef87438b8cc792b121340df32023-12-03T12:15:10ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542023-11-011611910.1186/s13068-023-02429-6Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid productionWilliam Woodruff0Narendra Naik Deshavath1Vionna Susanto2Christopher V. Rao3Vijay Singh4Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-ChampaignDepartment of Agricultural and Biological Engineering, University of Illinois at Urbana-ChampaignDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-ChampaignDepartment of Chemical and Biomolecular Engineering, University of Illinois at Urbana-ChampaignDepartment of Agricultural and Biological Engineering, University of Illinois at Urbana-ChampaignAbstract Background Oleaginous yeasts are a promising candidate for the sustainable conversion of lignocellulosic feedstocks into fuels and chemicals, but their growth on these substrates can be inhibited as a result of upstream pretreatment and enzymatic hydrolysis conditions. Previous studies indicate a high citrate buffer concentration during hydrolysis inhibits downstream cell growth and ethanol fermentation in Saccharomyces cerevisiae. In this study, an engineered Rhodosporidium toruloides strain with enhanced lipid accumulation was grown on sorghum hydrolysate with high and low citrate buffer concentrations. Results Both hydrolysis conditions resulted in similar sugar recovery rates and concentrations. No significant differences in cell growth, sugar utilization rates, or lipid production rates were observed between the two citrate buffer conditions during batch fermentation of R. toruloides. Under fed-batch growth on low-citrate hydrolysate a lipid titer of 16.7 g/L was obtained. Conclusions Citrate buffer was not found to inhibit growth or lipid production in this engineered R. toruloides strain, nor did reducing the citrate buffer concentration negatively affect sugar yields in the hydrolysate. As this process is scaled-up, $131 per ton of hydrothermally pretreated biomass can be saved by use of the lower citrate buffer concentration during enzymatic hydrolysis. Graphical Abstracthttps://doi.org/10.1186/s13068-023-02429-6Bioenergy sorghumCitrate bufferFermentationLipidsOleaginous yeastRhodosporidium toruloides |
| spellingShingle | William Woodruff Narendra Naik Deshavath Vionna Susanto Christopher V. Rao Vijay Singh Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid production Biotechnology for Biofuels and Bioproducts Bioenergy sorghum Citrate buffer Fermentation Lipids Oleaginous yeast Rhodosporidium toruloides |
| title | Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid production |
| title_full | Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid production |
| title_fullStr | Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid production |
| title_full_unstemmed | Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid production |
| title_short | Tolerance of engineered Rhodosporidium toruloides to sorghum hydrolysates during batch and fed-batch lipid production |
| title_sort | tolerance of engineered rhodosporidium toruloides to sorghum hydrolysates during batch and fed batch lipid production |
| topic | Bioenergy sorghum Citrate buffer Fermentation Lipids Oleaginous yeast Rhodosporidium toruloides |
| url | https://doi.org/10.1186/s13068-023-02429-6 |
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