Combined masked LCD-printing and microfabrication for bioimpedance-chips
Biomedical in vitro sensors use cell cultures grown on sensor chips for drug testing, toxicological screening, studying pathologic processes in tissue and for personalized medicine. Microfluidic systems and chips bridge the gap of the biological micro world to our accessible macro world, creating th...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-08-01
|
| Series: | Micro and Nano Engineering |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590007222000569 |
| _version_ | 1811339829925904384 |
|---|---|
| author | Julia Linert Philipp Taus Sonia Prado-López Markus Pribyl Samuele M. Dozio Michael J. Haslinger Elena Guillen Michael Muehlberger Heinz D. Wanzenboeck |
| author_facet | Julia Linert Philipp Taus Sonia Prado-López Markus Pribyl Samuele M. Dozio Michael J. Haslinger Elena Guillen Michael Muehlberger Heinz D. Wanzenboeck |
| author_sort | Julia Linert |
| collection | DOAJ |
| description | Biomedical in vitro sensors use cell cultures grown on sensor chips for drug testing, toxicological screening, studying pathologic processes in tissue and for personalized medicine. Microfluidic systems and chips bridge the gap of the biological micro world to our accessible macro world, creating the interface between e.g., cells on a chip to reservoirs and pumps. Prototype and low volume lab scale microfluidic devices have traditionally been realized by soft lithography using polydimethylsiloxane (PDMS) technology. Recently, rapid prototyping of microfluidic devices using direct 3D printing has become widely available. Usually, the 3D printed parts are (i) either stand-alone systems requiring only fluidic connections, or (ii) they need to be carefully aligned and skilfully attached to the rigid micro fabricated chip. This post-fabrication attachment is time-consuming and a frequent source of error. In this work the fabrication of the microchip and the microfluidic system have been integrated into a multi technology fabrication process. For the first time we demonstrate the “on-chip 3D printing” of a microfluidic attachment directly onto an in-house fabricated multi electrode array chip. The process uses a desktop-sized LCD resin printer and eliminates the time-consuming post-deposition alignment and attachment. Biocompatibility of the used resin was confirmed for murine fibroblasts and validates this multi technology approach for biomedical cell chips. |
| first_indexed | 2024-04-13T18:33:18Z |
| format | Article |
| id | doaj.art-84a68914ec3c44c6a6226949412a2aa9 |
| institution | Directory Open Access Journal |
| issn | 2590-0072 |
| language | English |
| last_indexed | 2024-04-13T18:33:18Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Micro and Nano Engineering |
| spelling | doaj.art-84a68914ec3c44c6a6226949412a2aa92022-12-22T02:35:00ZengElsevierMicro and Nano Engineering2590-00722022-08-0116100159Combined masked LCD-printing and microfabrication for bioimpedance-chipsJulia Linert0Philipp Taus1Sonia Prado-López2Markus Pribyl3Samuele M. Dozio4Michael J. Haslinger5Elena Guillen6Michael Muehlberger7Heinz D. Wanzenboeck8TU Wien, Institute of Solid State Electronics, Vienna A-1040, Austria; TU Wien, Institute of Applied Physics, Vienna A-1040, AustriaTU Wien, Institute of Solid State Electronics, Vienna A-1040, AustriaTU Wien, Institute of Solid State Electronics, Vienna A-1040, AustriaTU Wien, Institute of Solid State Electronics, Vienna A-1040, AustriaTU Wien, Institute of Solid State Electronics, Vienna A-1040, AustriaPROFACTOR GmbH, Steyr 4407, AustriaPROFACTOR GmbH, Steyr 4407, AustriaPROFACTOR GmbH, Steyr 4407, AustriaTU Wien, Institute of Solid State Electronics, Vienna A-1040, Austria; Corresponding author.Biomedical in vitro sensors use cell cultures grown on sensor chips for drug testing, toxicological screening, studying pathologic processes in tissue and for personalized medicine. Microfluidic systems and chips bridge the gap of the biological micro world to our accessible macro world, creating the interface between e.g., cells on a chip to reservoirs and pumps. Prototype and low volume lab scale microfluidic devices have traditionally been realized by soft lithography using polydimethylsiloxane (PDMS) technology. Recently, rapid prototyping of microfluidic devices using direct 3D printing has become widely available. Usually, the 3D printed parts are (i) either stand-alone systems requiring only fluidic connections, or (ii) they need to be carefully aligned and skilfully attached to the rigid micro fabricated chip. This post-fabrication attachment is time-consuming and a frequent source of error. In this work the fabrication of the microchip and the microfluidic system have been integrated into a multi technology fabrication process. For the first time we demonstrate the “on-chip 3D printing” of a microfluidic attachment directly onto an in-house fabricated multi electrode array chip. The process uses a desktop-sized LCD resin printer and eliminates the time-consuming post-deposition alignment and attachment. Biocompatibility of the used resin was confirmed for murine fibroblasts and validates this multi technology approach for biomedical cell chips.http://www.sciencedirect.com/science/article/pii/S2590007222000569Microfluidic device3D printingOn-Chip printingBiocompatibility |
| spellingShingle | Julia Linert Philipp Taus Sonia Prado-López Markus Pribyl Samuele M. Dozio Michael J. Haslinger Elena Guillen Michael Muehlberger Heinz D. Wanzenboeck Combined masked LCD-printing and microfabrication for bioimpedance-chips Micro and Nano Engineering Microfluidic device 3D printing On-Chip printing Biocompatibility |
| title | Combined masked LCD-printing and microfabrication for bioimpedance-chips |
| title_full | Combined masked LCD-printing and microfabrication for bioimpedance-chips |
| title_fullStr | Combined masked LCD-printing and microfabrication for bioimpedance-chips |
| title_full_unstemmed | Combined masked LCD-printing and microfabrication for bioimpedance-chips |
| title_short | Combined masked LCD-printing and microfabrication for bioimpedance-chips |
| title_sort | combined masked lcd printing and microfabrication for bioimpedance chips |
| topic | Microfluidic device 3D printing On-Chip printing Biocompatibility |
| url | http://www.sciencedirect.com/science/article/pii/S2590007222000569 |
| work_keys_str_mv | AT julialinert combinedmaskedlcdprintingandmicrofabricationforbioimpedancechips AT philipptaus combinedmaskedlcdprintingandmicrofabricationforbioimpedancechips AT soniapradolopez combinedmaskedlcdprintingandmicrofabricationforbioimpedancechips AT markuspribyl combinedmaskedlcdprintingandmicrofabricationforbioimpedancechips AT samuelemdozio combinedmaskedlcdprintingandmicrofabricationforbioimpedancechips AT michaeljhaslinger combinedmaskedlcdprintingandmicrofabricationforbioimpedancechips AT elenaguillen combinedmaskedlcdprintingandmicrofabricationforbioimpedancechips AT michaelmuehlberger combinedmaskedlcdprintingandmicrofabricationforbioimpedancechips AT heinzdwanzenboeck combinedmaskedlcdprintingandmicrofabricationforbioimpedancechips |