In Vitro Release of Glycyrrhiza Glabra Extract by a Gel-Based Microneedle Patch for Psoriasis Treatment
Microneedle patches are attractive drug delivery systems that give hope for treating skin disorders. In this study, to first fabricate a chitosan-based low-cost microneedle patch (MNP) using a CO<sub>2</sub> laser cutter for in vitro purposes was tried and then the delivery and impact of...
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/2310-2861/10/2/87 |
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| author | Ayeh Khorshidian Niloufar Sharifi Fatemeh Choupani Kheirabadi Farnoushsadat Rezaei Seyed Alireza Sheikholeslami Ayda Ariyannejad Javad Esmaeili Hojat Basati Aboulfazl Barati |
| author_facet | Ayeh Khorshidian Niloufar Sharifi Fatemeh Choupani Kheirabadi Farnoushsadat Rezaei Seyed Alireza Sheikholeslami Ayda Ariyannejad Javad Esmaeili Hojat Basati Aboulfazl Barati |
| author_sort | Ayeh Khorshidian |
| collection | DOAJ |
| description | Microneedle patches are attractive drug delivery systems that give hope for treating skin disorders. In this study, to first fabricate a chitosan-based low-cost microneedle patch (MNP) using a CO<sub>2</sub> laser cutter for in vitro purposes was tried and then the delivery and impact of Glycyrrhiza glabra extract (GgE) on the cell population by this microneedle was evaluated. Microscopic analysis, swelling, penetration, degradation, biocompatibility, and drug delivery were carried out to assess the patch’s performance. DAPI staining and acridine orange (AO) staining were performed to evaluate cell numbers. Based on the results, the MNs were conical and sharp enough (diameter: 400–500 μm, height: 700–900 μm). They showed notable swelling (2 folds) during 5 min and good degradability during 30 min, which can be considered a burst release. The MNP showed no cytotoxicity against fibroblast cell line L929. It also demonstrated good potential for GgE delivery. The results from AO and DAPI staining approved the reduction in the cell population after GgE delivery. To sum up, the fabricated MNP can be a useful recommendation for lab-scale studies. In addition, a GgE-loaded MNP can be a good remedy for skin disorders in which cell proliferation needs to be controlled. |
| first_indexed | 2024-03-07T22:31:44Z |
| format | Article |
| id | doaj.art-84a967bdd9994eb5a0cfc07a547307b0 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-07T22:31:44Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-84a967bdd9994eb5a0cfc07a547307b02024-02-23T15:17:25ZengMDPI AGGels2310-28612024-01-011028710.3390/gels10020087In Vitro Release of Glycyrrhiza Glabra Extract by a Gel-Based Microneedle Patch for Psoriasis TreatmentAyeh Khorshidian0Niloufar Sharifi1Fatemeh Choupani Kheirabadi2Farnoushsadat Rezaei3Seyed Alireza Sheikholeslami4Ayda Ariyannejad5Javad Esmaeili6Hojat Basati7Aboulfazl Barati8Department of Biomedical Engineering, TISSUEHUB Co., Tehran 1956854977, IranDepartment of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, IranDepartment of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, IranDepartment of Chemical and Biomedical Engineering, University of Missouri, Columbia, MO 65211, USADepartment of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, IranDepartment of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, IranDepartment of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, IranDepartment of Tissue Engineering, TISSUEHUB Co., Tehran 1956854977, IranCenter for Materials and Manufacturing Sciences, Department of Chemistry and Physics, Troy University, Troy, AL 36082, USAMicroneedle patches are attractive drug delivery systems that give hope for treating skin disorders. In this study, to first fabricate a chitosan-based low-cost microneedle patch (MNP) using a CO<sub>2</sub> laser cutter for in vitro purposes was tried and then the delivery and impact of Glycyrrhiza glabra extract (GgE) on the cell population by this microneedle was evaluated. Microscopic analysis, swelling, penetration, degradation, biocompatibility, and drug delivery were carried out to assess the patch’s performance. DAPI staining and acridine orange (AO) staining were performed to evaluate cell numbers. Based on the results, the MNs were conical and sharp enough (diameter: 400–500 μm, height: 700–900 μm). They showed notable swelling (2 folds) during 5 min and good degradability during 30 min, which can be considered a burst release. The MNP showed no cytotoxicity against fibroblast cell line L929. It also demonstrated good potential for GgE delivery. The results from AO and DAPI staining approved the reduction in the cell population after GgE delivery. To sum up, the fabricated MNP can be a useful recommendation for lab-scale studies. In addition, a GgE-loaded MNP can be a good remedy for skin disorders in which cell proliferation needs to be controlled.https://www.mdpi.com/2310-2861/10/2/87microneedle patchskin disorderdrug deliveryburst releasepsoriasis |
| spellingShingle | Ayeh Khorshidian Niloufar Sharifi Fatemeh Choupani Kheirabadi Farnoushsadat Rezaei Seyed Alireza Sheikholeslami Ayda Ariyannejad Javad Esmaeili Hojat Basati Aboulfazl Barati In Vitro Release of Glycyrrhiza Glabra Extract by a Gel-Based Microneedle Patch for Psoriasis Treatment Gels microneedle patch skin disorder drug delivery burst release psoriasis |
| title | In Vitro Release of Glycyrrhiza Glabra Extract by a Gel-Based Microneedle Patch for Psoriasis Treatment |
| title_full | In Vitro Release of Glycyrrhiza Glabra Extract by a Gel-Based Microneedle Patch for Psoriasis Treatment |
| title_fullStr | In Vitro Release of Glycyrrhiza Glabra Extract by a Gel-Based Microneedle Patch for Psoriasis Treatment |
| title_full_unstemmed | In Vitro Release of Glycyrrhiza Glabra Extract by a Gel-Based Microneedle Patch for Psoriasis Treatment |
| title_short | In Vitro Release of Glycyrrhiza Glabra Extract by a Gel-Based Microneedle Patch for Psoriasis Treatment |
| title_sort | in vitro release of glycyrrhiza glabra extract by a gel based microneedle patch for psoriasis treatment |
| topic | microneedle patch skin disorder drug delivery burst release psoriasis |
| url | https://www.mdpi.com/2310-2861/10/2/87 |
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