Investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant Senna italica and their role as plant growth promoting factors
Natural microbial communities associated with desert plants are found in soils that face nutrient deficiencies and extreme environments, including salinity and drought. In this study, 16S rRNA metagenomic sequencing was used to screen and identify bacterial assemblies associated with the desert pla...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AcademicPres
2023-02-01
|
| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| Subjects: | |
| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13053 |
| _version_ | 1797854742167158784 |
|---|---|
| author | Asrar M. ALSWAT Rewaa S. JALAL Ashwag Y. SHAMI Mazen A. MAJEED Zohoor S. ALSAEDI Lina BAZ Mohammed Y. REFAI Mohammed N. BAESHEN Noor M. BATAWEEL Ahmed AL-HEJIN Ruba A. ASHY |
| author_facet | Asrar M. ALSWAT Rewaa S. JALAL Ashwag Y. SHAMI Mazen A. MAJEED Zohoor S. ALSAEDI Lina BAZ Mohammed Y. REFAI Mohammed N. BAESHEN Noor M. BATAWEEL Ahmed AL-HEJIN Ruba A. ASHY |
| author_sort | Asrar M. ALSWAT |
| collection | DOAJ |
| description |
Natural microbial communities associated with desert plants are found in soils that face nutrient deficiencies and extreme environments, including salinity and drought. In this study, 16S rRNA metagenomic sequencing was used to screen and identify bacterial assemblies associated with the desert plant Senna italica, obtained from diverse soil samples located in the Asfan region, northeast of Jeddah, Saudi Arabia. Several studies found Senna italica as a valuable medicinal plant for treating different diseases; however, a few studies were done on its association with bacterial communities under drought conditions. This study aimed to identify bacterial communities present in the drought soil environment of the Senna italica plants. To approach our goals, we applied metagenomic techniques, discovering a new bacterial strain beneficial for biotechnological applications. Our results showed that the analysis of the 16S rRNA sequences at the taxonomic phylum level detected 15 phyla of bacterial populations in the soil samples. The most prevalent was kept for further research. Our findings demonstrated that rhizospheric bacteria may be used as indicators of plant growth rate and survival ability in hostile environments. Studying the soil microbiome's taxonomic, phylogenetic, and functional diversity will facilitate identifying new candidates for biological agents that can be used to improve agricultural and industrial processes.
|
| first_indexed | 2024-04-09T20:12:10Z |
| format | Article |
| id | doaj.art-84ad0a4e77d64405ade410aef6608769 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-04-09T20:12:10Z |
| publishDate | 2023-02-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-84ad0a4e77d64405ade410aef66087692023-03-31T16:41:03ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092023-02-0151110.15835/nbha51113053Investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant Senna italica and their role as plant growth promoting factorsAsrar M. ALSWAT0Rewaa S. JALAL1Ashwag Y. SHAMI2Mazen A. MAJEED 3Zohoor S. ALSAEDI4Lina BAZ5Mohammed Y. REFAI6Mohammed N. BAESHEN7Noor M. BATAWEEL8Ahmed AL-HEJIN9Ruba A. ASHY 10University of Jeddah, College of Science, Department of Biology, Jeddah 21493University of Jeddah, College of Science, Department of Biology, Jeddah 21493Princess Nourah bint Abdulrahman University, College of Sciences, Department of Biology, P.O. Box 84428, Riyadh 11671University of Jeddah, College of Science, Department of Biology, Jeddah 21493University of Jeddah, College of Science, Department of Biology, Jeddah 21493King Abdulaziz University, Faculty of Science, Department of Biochemistry, Jeddah 21589University of Jeddah, College of Science, Department of Biochemistry, Jeddah 21493University of Jeddah, College of Science, Department of Biology, Jeddah 21493King Fahad Medical Research Center, P.O. Box 80216, King Abdulaziz University, Jeddah, 21589King Abdulaziz University, Faculty of Science, Department of Biological Sciences, JeddahUniversity of Jeddah, College of Science, Department of Biology, Jeddah 21493 Natural microbial communities associated with desert plants are found in soils that face nutrient deficiencies and extreme environments, including salinity and drought. In this study, 16S rRNA metagenomic sequencing was used to screen and identify bacterial assemblies associated with the desert plant Senna italica, obtained from diverse soil samples located in the Asfan region, northeast of Jeddah, Saudi Arabia. Several studies found Senna italica as a valuable medicinal plant for treating different diseases; however, a few studies were done on its association with bacterial communities under drought conditions. This study aimed to identify bacterial communities present in the drought soil environment of the Senna italica plants. To approach our goals, we applied metagenomic techniques, discovering a new bacterial strain beneficial for biotechnological applications. Our results showed that the analysis of the 16S rRNA sequences at the taxonomic phylum level detected 15 phyla of bacterial populations in the soil samples. The most prevalent was kept for further research. Our findings demonstrated that rhizospheric bacteria may be used as indicators of plant growth rate and survival ability in hostile environments. Studying the soil microbiome's taxonomic, phylogenetic, and functional diversity will facilitate identifying new candidates for biological agents that can be used to improve agricultural and industrial processes. https://www.notulaebotanicae.ro/index.php/nbha/article/view/13053metagenomicsmicrobiomePGPRrhizosphereSenna italica plant |
| spellingShingle | Asrar M. ALSWAT Rewaa S. JALAL Ashwag Y. SHAMI Mazen A. MAJEED Zohoor S. ALSAEDI Lina BAZ Mohammed Y. REFAI Mohammed N. BAESHEN Noor M. BATAWEEL Ahmed AL-HEJIN Ruba A. ASHY Investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant Senna italica and their role as plant growth promoting factors Notulae Botanicae Horti Agrobotanici Cluj-Napoca metagenomics microbiome PGPR rhizosphere Senna italica plant |
| title | Investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant Senna italica and their role as plant growth promoting factors |
| title_full | Investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant Senna italica and their role as plant growth promoting factors |
| title_fullStr | Investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant Senna italica and their role as plant growth promoting factors |
| title_full_unstemmed | Investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant Senna italica and their role as plant growth promoting factors |
| title_short | Investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant Senna italica and their role as plant growth promoting factors |
| title_sort | investigating the metagenomics of the bacterial communities in the rhizosphere of the desert plant senna italica and their role as plant growth promoting factors |
| topic | metagenomics microbiome PGPR rhizosphere Senna italica plant |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13053 |
| work_keys_str_mv | AT asrarmalswat investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors AT rewaasjalal investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors AT ashwagyshami investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors AT mazenamajeed investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors AT zohoorsalsaedi investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors AT linabaz investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors AT mohammedyrefai investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors AT mohammednbaeshen investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors AT noormbataweel investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors AT ahmedalhejin investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors AT rubaaashy investigatingthemetagenomicsofthebacterialcommunitiesintherhizosphereofthedesertplantsennaitalicaandtheirroleasplantgrowthpromotingfactors |