Telomere Dynamics in the Diploid and Triploid Rainbow Trout (<i>Oncorhynchus mykiss</i>) Assessed by Q-FISH Analysis
Changes of telomere length with age were assessed in diploid and triploid rainbow trout <i>(Oncorhynchus mykiss</i>) females in the cross-sectional study using Q-FISH technique. Triploid trout as sterile do not invest an energy in gametogenesis and continue to grow, whereas fertile diplo...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/11/7/786 |
| _version_ | 1797416943868706816 |
|---|---|
| author | Ligia Panasiak Stefan Dobosz Konrad Ocalewicz |
| author_facet | Ligia Panasiak Stefan Dobosz Konrad Ocalewicz |
| author_sort | Ligia Panasiak |
| collection | DOAJ |
| description | Changes of telomere length with age were assessed in diploid and triploid rainbow trout <i>(Oncorhynchus mykiss</i>) females in the cross-sectional study using Q-FISH technique. Triploid trout as sterile do not invest an energy in gametogenesis and continue to grow, whereas fertile diploid individuals suffer from declines in growth and survival during sexual maturation. However, triploid and diploid specimens exhibited similar patterns of telomere dynamics. Telomere length in the embryos, larvae and one-year-old juveniles did not change significantly. In the second year after hatching, subadults exhibited substantially shortened telomeres, while significant increase of the telomere length was reported in the three-year-old adults. On the other hand, correlation between telomere length and body size was observed in the triploid, but not in the diploid rainbow trout. Telomere shortening observed in two-year-old subadults may have been associated with the premature period of the fast growth in rainbow trout. Similar pattern of the telomere dynamics reported in the fertile diploids and sterile triploids indicated processes related to reproduction did not affect telomere dynamics in this species. Unexpected increase of the telomere length reported during the third year of life confirmed that in rainbow trout telomeric DNA shortens and lengthens, depending on the developmental stage. |
| first_indexed | 2024-03-09T06:11:41Z |
| format | Article |
| id | doaj.art-84ae04afec104cdbb6da993322bf28e6 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T06:11:41Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-84ae04afec104cdbb6da993322bf28e62023-12-03T11:57:45ZengMDPI AGGenes2073-44252020-07-0111778610.3390/genes11070786Telomere Dynamics in the Diploid and Triploid Rainbow Trout (<i>Oncorhynchus mykiss</i>) Assessed by Q-FISH AnalysisLigia Panasiak0Stefan Dobosz1Konrad Ocalewicz2Department of Marine Biology and Ecology, Institute of Oceanography, Faculty of Oceanography and Geography, University of Gdansk, M. Piłsudskiego 46 Av, 81378 Gdynia, PolandDepartment of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki, 10719 Olsztyn, PolandDepartment of Marine Biology and Ecology, Institute of Oceanography, Faculty of Oceanography and Geography, University of Gdansk, M. Piłsudskiego 46 Av, 81378 Gdynia, PolandChanges of telomere length with age were assessed in diploid and triploid rainbow trout <i>(Oncorhynchus mykiss</i>) females in the cross-sectional study using Q-FISH technique. Triploid trout as sterile do not invest an energy in gametogenesis and continue to grow, whereas fertile diploid individuals suffer from declines in growth and survival during sexual maturation. However, triploid and diploid specimens exhibited similar patterns of telomere dynamics. Telomere length in the embryos, larvae and one-year-old juveniles did not change significantly. In the second year after hatching, subadults exhibited substantially shortened telomeres, while significant increase of the telomere length was reported in the three-year-old adults. On the other hand, correlation between telomere length and body size was observed in the triploid, but not in the diploid rainbow trout. Telomere shortening observed in two-year-old subadults may have been associated with the premature period of the fast growth in rainbow trout. Similar pattern of the telomere dynamics reported in the fertile diploids and sterile triploids indicated processes related to reproduction did not affect telomere dynamics in this species. Unexpected increase of the telomere length reported during the third year of life confirmed that in rainbow trout telomeric DNA shortens and lengthens, depending on the developmental stage.https://www.mdpi.com/2073-4425/11/7/786aginggrowthtelomere attrition |
| spellingShingle | Ligia Panasiak Stefan Dobosz Konrad Ocalewicz Telomere Dynamics in the Diploid and Triploid Rainbow Trout (<i>Oncorhynchus mykiss</i>) Assessed by Q-FISH Analysis Genes aging growth telomere attrition |
| title | Telomere Dynamics in the Diploid and Triploid Rainbow Trout (<i>Oncorhynchus mykiss</i>) Assessed by Q-FISH Analysis |
| title_full | Telomere Dynamics in the Diploid and Triploid Rainbow Trout (<i>Oncorhynchus mykiss</i>) Assessed by Q-FISH Analysis |
| title_fullStr | Telomere Dynamics in the Diploid and Triploid Rainbow Trout (<i>Oncorhynchus mykiss</i>) Assessed by Q-FISH Analysis |
| title_full_unstemmed | Telomere Dynamics in the Diploid and Triploid Rainbow Trout (<i>Oncorhynchus mykiss</i>) Assessed by Q-FISH Analysis |
| title_short | Telomere Dynamics in the Diploid and Triploid Rainbow Trout (<i>Oncorhynchus mykiss</i>) Assessed by Q-FISH Analysis |
| title_sort | telomere dynamics in the diploid and triploid rainbow trout i oncorhynchus mykiss i assessed by q fish analysis |
| topic | aging growth telomere attrition |
| url | https://www.mdpi.com/2073-4425/11/7/786 |
| work_keys_str_mv | AT ligiapanasiak telomeredynamicsinthediploidandtriploidrainbowtroutioncorhynchusmykissiassessedbyqfishanalysis AT stefandobosz telomeredynamicsinthediploidandtriploidrainbowtroutioncorhynchusmykissiassessedbyqfishanalysis AT konradocalewicz telomeredynamicsinthediploidandtriploidrainbowtroutioncorhynchusmykissiassessedbyqfishanalysis |