Recent Advances in Single Cell Analysis by Electrochemiluminescence
Abstract Understanding biological mechanisms operating in cells is one of the major goals of biology. Since heterogeneity is the fundamental property of cellular systems, single cell measurements can provide more accurate information about the composition, dynamics, and regulatory circuits of cells...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-05-01
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| Series: | ChemistryOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/open.202200113 |
| _version_ | 1797819960172478464 |
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| author | Dr. Hao Ding Prof. Bin Su Prof. Dechen Jiang |
| author_facet | Dr. Hao Ding Prof. Bin Su Prof. Dechen Jiang |
| author_sort | Dr. Hao Ding |
| collection | DOAJ |
| description | Abstract Understanding biological mechanisms operating in cells is one of the major goals of biology. Since heterogeneity is the fundamental property of cellular systems, single cell measurements can provide more accurate information about the composition, dynamics, and regulatory circuits of cells than population‐averaged assays. Electrochemiluminescence (ECL), the light emission triggered by electrochemical reactions, is an emerging approach for single cell analysis. Numerous analytes, ranging from small biomolecules such as glucose and cholesterol, proteins and nucleic acids to subcellular structures, have been determined in single cells by ECL, which yields new insights into cellular functions. This review aims to provide an overview of research progress on ECL principles and systems for single cell analysis in recent years. The ECL reaction mechanisms are briefly introduced, and then the advances and representative works in ECL single cell analysis are summarized. Finally, outlooks and challenges in this field are addressed. |
| first_indexed | 2024-03-13T09:30:19Z |
| format | Article |
| id | doaj.art-84b02210b5534f36bceb697df7f1978b |
| institution | Directory Open Access Journal |
| issn | 2191-1363 |
| language | English |
| last_indexed | 2024-03-13T09:30:19Z |
| publishDate | 2023-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | ChemistryOpen |
| spelling | doaj.art-84b02210b5534f36bceb697df7f1978b2023-05-26T04:02:37ZengWiley-VCHChemistryOpen2191-13632023-05-01125n/an/a10.1002/open.202200113Recent Advances in Single Cell Analysis by ElectrochemiluminescenceDr. Hao Ding0Prof. Bin Su1Prof. Dechen Jiang2State Key Laboratory of Analytical Chemistry for Life Science Chemistry and Biomedicine Innovation Center (ChemBIC) School of Chemistry and Chemical Engineering Nanjing University Nanjing Jiangsu 210023 ChinaInstitute of Analytical Chemistry Department of Chemistry Zhejiang University Hangzhou Zhejiang 310058 ChinaState Key Laboratory of Analytical Chemistry for Life Science Chemistry and Biomedicine Innovation Center (ChemBIC) School of Chemistry and Chemical Engineering Nanjing University Nanjing Jiangsu 210023 ChinaAbstract Understanding biological mechanisms operating in cells is one of the major goals of biology. Since heterogeneity is the fundamental property of cellular systems, single cell measurements can provide more accurate information about the composition, dynamics, and regulatory circuits of cells than population‐averaged assays. Electrochemiluminescence (ECL), the light emission triggered by electrochemical reactions, is an emerging approach for single cell analysis. Numerous analytes, ranging from small biomolecules such as glucose and cholesterol, proteins and nucleic acids to subcellular structures, have been determined in single cells by ECL, which yields new insights into cellular functions. This review aims to provide an overview of research progress on ECL principles and systems for single cell analysis in recent years. The ECL reaction mechanisms are briefly introduced, and then the advances and representative works in ECL single cell analysis are summarized. Finally, outlooks and challenges in this field are addressed.https://doi.org/10.1002/open.202200113electrochemiluminescencesingle cellsreaction mechanismsbiomoleculescellular structures |
| spellingShingle | Dr. Hao Ding Prof. Bin Su Prof. Dechen Jiang Recent Advances in Single Cell Analysis by Electrochemiluminescence ChemistryOpen electrochemiluminescence single cells reaction mechanisms biomolecules cellular structures |
| title | Recent Advances in Single Cell Analysis by Electrochemiluminescence |
| title_full | Recent Advances in Single Cell Analysis by Electrochemiluminescence |
| title_fullStr | Recent Advances in Single Cell Analysis by Electrochemiluminescence |
| title_full_unstemmed | Recent Advances in Single Cell Analysis by Electrochemiluminescence |
| title_short | Recent Advances in Single Cell Analysis by Electrochemiluminescence |
| title_sort | recent advances in single cell analysis by electrochemiluminescence |
| topic | electrochemiluminescence single cells reaction mechanisms biomolecules cellular structures |
| url | https://doi.org/10.1002/open.202200113 |
| work_keys_str_mv | AT drhaoding recentadvancesinsinglecellanalysisbyelectrochemiluminescence AT profbinsu recentadvancesinsinglecellanalysisbyelectrochemiluminescence AT profdechenjiang recentadvancesinsinglecellanalysisbyelectrochemiluminescence |