Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing of Stipa grandis
Heavy grazing significantly reduces Stipa grandis growth. To enhance our understanding of plant responses to heavy grazing, we conducted transcriptomic, proteomic, and metabolic analyses of the leaves of non-grazed plants (NG) and heavy-grazing-induced dwarf plants (HG) of S. grandis. A total of 101...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.995074/full |
| _version_ | 1811235217312055296 |
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| author | Dongli Wan Yongqing Wan Tongrui Zhang Ruigang Wang Yong Ding |
| author_facet | Dongli Wan Yongqing Wan Tongrui Zhang Ruigang Wang Yong Ding |
| author_sort | Dongli Wan |
| collection | DOAJ |
| description | Heavy grazing significantly reduces Stipa grandis growth. To enhance our understanding of plant responses to heavy grazing, we conducted transcriptomic, proteomic, and metabolic analyses of the leaves of non-grazed plants (NG) and heavy-grazing-induced dwarf plants (HG) of S. grandis. A total of 101 metabolites, 167 proteins, and 1,268 genes differed in abundance between the HG and NG groups. Analysis of Kyoto Encyclopedia of Genes and Genomes pathways among differentially accumulated metabolites (DAMs) revealed that the most enriched pathways were flavone and flavonol biosynthesis, tryptophan metabolism, and phenylpropanoid biosynthesis. An integrative analysis of differentially expressed genes (DEGs) and proteins, and DAMs in these three pathways was performed. Heavy-grazing-induced dwarfism decreased the accumulation of DAMs enriched in phenylpropanoid biosynthesis, among which four DAMs were associated with lignin biosynthesis. In contrast, all DAMs enriched in flavone and flavonol biosynthesis and tryptophan metabolism showed increased accumulation in HG compared with NG plants. Among the DAMs enriched in tryptophan metabolism, three were involved in tryptophan-dependent IAA biosynthesis. Some of the DEGs and proteins enriched in these pathways showed different expression trends. The results indicated that these pathways play important roles in the regulation of growth and grazing-associated stress adaptions of S. grandis. This study enriches the knowledge of the mechanism of heavy-grazing-induced growth inhibition of S. grandis and provides valuable information for restoration of the productivity in degraded grassland. |
| first_indexed | 2024-04-12T11:47:19Z |
| format | Article |
| id | doaj.art-84b084e9356f4c1ab095f1cd28b4c00c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T11:47:19Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-84b084e9356f4c1ab095f1cd28b4c00c2022-12-22T03:34:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.995074995074Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing of Stipa grandisDongli Wan0Yongqing Wan1Tongrui Zhang2Ruigang Wang3Yong Ding4Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, ChinaCollege of Life Sciences, Inner Mongolia Agricultural University, Hohhot, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, ChinaCollege of Life Sciences, Inner Mongolia Agricultural University, Hohhot, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, ChinaHeavy grazing significantly reduces Stipa grandis growth. To enhance our understanding of plant responses to heavy grazing, we conducted transcriptomic, proteomic, and metabolic analyses of the leaves of non-grazed plants (NG) and heavy-grazing-induced dwarf plants (HG) of S. grandis. A total of 101 metabolites, 167 proteins, and 1,268 genes differed in abundance between the HG and NG groups. Analysis of Kyoto Encyclopedia of Genes and Genomes pathways among differentially accumulated metabolites (DAMs) revealed that the most enriched pathways were flavone and flavonol biosynthesis, tryptophan metabolism, and phenylpropanoid biosynthesis. An integrative analysis of differentially expressed genes (DEGs) and proteins, and DAMs in these three pathways was performed. Heavy-grazing-induced dwarfism decreased the accumulation of DAMs enriched in phenylpropanoid biosynthesis, among which four DAMs were associated with lignin biosynthesis. In contrast, all DAMs enriched in flavone and flavonol biosynthesis and tryptophan metabolism showed increased accumulation in HG compared with NG plants. Among the DAMs enriched in tryptophan metabolism, three were involved in tryptophan-dependent IAA biosynthesis. Some of the DEGs and proteins enriched in these pathways showed different expression trends. The results indicated that these pathways play important roles in the regulation of growth and grazing-associated stress adaptions of S. grandis. This study enriches the knowledge of the mechanism of heavy-grazing-induced growth inhibition of S. grandis and provides valuable information for restoration of the productivity in degraded grassland.https://www.frontiersin.org/articles/10.3389/fpls.2022.995074/fullStipa grandisheavy grazingtranscriptomicproteomicmetabolic |
| spellingShingle | Dongli Wan Yongqing Wan Tongrui Zhang Ruigang Wang Yong Ding Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing of Stipa grandis Frontiers in Plant Science Stipa grandis heavy grazing transcriptomic proteomic metabolic |
| title | Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing of Stipa grandis |
| title_full | Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing of Stipa grandis |
| title_fullStr | Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing of Stipa grandis |
| title_full_unstemmed | Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing of Stipa grandis |
| title_short | Multi-omics analysis reveals the molecular changes accompanying heavy-grazing-induced dwarfing of Stipa grandis |
| title_sort | multi omics analysis reveals the molecular changes accompanying heavy grazing induced dwarfing of stipa grandis |
| topic | Stipa grandis heavy grazing transcriptomic proteomic metabolic |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.995074/full |
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