Analysis of Complete Genome Sequence of <i>Acinetobacter</i> <i>baumannii</i> Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage
<i>Acinetobacter baumannii</i> isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of <i>A. baumannii</i>, while, until recently, a complete genome of thi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/8/12/1851 |
| _version_ | 1827701572171202560 |
|---|---|
| author | Mohammad Hamidian Lucia Blasco Lauren N. Tillman Joyce To María Tomas Garry S. A. Myers |
| author_facet | Mohammad Hamidian Lucia Blasco Lauren N. Tillman Joyce To María Tomas Garry S. A. Myers |
| author_sort | Mohammad Hamidian |
| collection | DOAJ |
| description | <i>Acinetobacter baumannii</i> isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of <i>A. baumannii</i>, while, until recently, a complete genome of this strain was not available. Here, we present an analysis of the complete 3.91-Mbp genome sequence, generated via a combination of short-read sequencing (Illumina) and long-read sequencing (MinION), and show it contains two small cryptic plasmids and a novel complete prophage of size 41.2 kb. We also characterised several regions of the ATCC 19606 genome, leading to the identification of a novel cadmium/mercury transposon, which was named Tn<i>6551</i>. ATCC 19606 is an antibiotic-sensitive strain, but a comparative analysis of all publicly available ST52 strains predicts a resistance to modern antibiotics by the accumulation of antibiotic-resistance genes via plasmids in recent isolates that belong to this sequence type. |
| first_indexed | 2024-03-10T14:37:02Z |
| format | Article |
| id | doaj.art-84b330fbdcea4b1b9edeb49f11bb73f1 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:37:02Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-84b330fbdcea4b1b9edeb49f11bb73f12023-11-20T22:07:41ZengMDPI AGMicroorganisms2076-26072020-11-01812185110.3390/microorganisms8121851Analysis of Complete Genome Sequence of <i>Acinetobacter</i> <i>baumannii</i> Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel ProphageMohammad Hamidian0Lucia Blasco1Lauren N. Tillman2Joyce To3María Tomas4Garry S. A. Myers5The iThree Institute, University of Technology Sydney, Ultimo 2007, NSW, AustraliaMicrobiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), 15495 A Coruña, SpainThe iThree Institute, University of Technology Sydney, Ultimo 2007, NSW, AustraliaThe iThree Institute, University of Technology Sydney, Ultimo 2007, NSW, AustraliaMicrobiology Department-Research Institute Biomedical A Coruña (INIBIC), Hospital A Coruña (CHUAC), University of A Coruña (UDC), 15495 A Coruña, SpainThe iThree Institute, University of Technology Sydney, Ultimo 2007, NSW, Australia<i>Acinetobacter baumannii</i> isolate ATCC 19606 was recovered in the US prior to 1948. It has been used as a reference and model organism in many studies involving antibiotic resistance and pathogenesis of <i>A. baumannii</i>, while, until recently, a complete genome of this strain was not available. Here, we present an analysis of the complete 3.91-Mbp genome sequence, generated via a combination of short-read sequencing (Illumina) and long-read sequencing (MinION), and show it contains two small cryptic plasmids and a novel complete prophage of size 41.2 kb. We also characterised several regions of the ATCC 19606 genome, leading to the identification of a novel cadmium/mercury transposon, which was named Tn<i>6551</i>. ATCC 19606 is an antibiotic-sensitive strain, but a comparative analysis of all publicly available ST52 strains predicts a resistance to modern antibiotics by the accumulation of antibiotic-resistance genes via plasmids in recent isolates that belong to this sequence type.https://www.mdpi.com/2076-2607/8/12/1851<i>Acinetobacter baumannii</i> ATCC 19606complete genomeplasmidantibiotic resistanceST52 and Multi-Locus Sequence Typing (MLST) |
| spellingShingle | Mohammad Hamidian Lucia Blasco Lauren N. Tillman Joyce To María Tomas Garry S. A. Myers Analysis of Complete Genome Sequence of <i>Acinetobacter</i> <i>baumannii</i> Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage Microorganisms <i>Acinetobacter baumannii</i> ATCC 19606 complete genome plasmid antibiotic resistance ST52 and Multi-Locus Sequence Typing (MLST) |
| title | Analysis of Complete Genome Sequence of <i>Acinetobacter</i> <i>baumannii</i> Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
| title_full | Analysis of Complete Genome Sequence of <i>Acinetobacter</i> <i>baumannii</i> Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
| title_fullStr | Analysis of Complete Genome Sequence of <i>Acinetobacter</i> <i>baumannii</i> Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
| title_full_unstemmed | Analysis of Complete Genome Sequence of <i>Acinetobacter</i> <i>baumannii</i> Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
| title_short | Analysis of Complete Genome Sequence of <i>Acinetobacter</i> <i>baumannii</i> Strain ATCC 19606 Reveals Novel Mobile Genetic Elements and Novel Prophage |
| title_sort | analysis of complete genome sequence of i acinetobacter i i baumannii i strain atcc 19606 reveals novel mobile genetic elements and novel prophage |
| topic | <i>Acinetobacter baumannii</i> ATCC 19606 complete genome plasmid antibiotic resistance ST52 and Multi-Locus Sequence Typing (MLST) |
| url | https://www.mdpi.com/2076-2607/8/12/1851 |
| work_keys_str_mv | AT mohammadhamidian analysisofcompletegenomesequenceofiacinetobacteriibaumanniiistrainatcc19606revealsnovelmobilegeneticelementsandnovelprophage AT luciablasco analysisofcompletegenomesequenceofiacinetobacteriibaumanniiistrainatcc19606revealsnovelmobilegeneticelementsandnovelprophage AT laurenntillman analysisofcompletegenomesequenceofiacinetobacteriibaumanniiistrainatcc19606revealsnovelmobilegeneticelementsandnovelprophage AT joyceto analysisofcompletegenomesequenceofiacinetobacteriibaumanniiistrainatcc19606revealsnovelmobilegeneticelementsandnovelprophage AT mariatomas analysisofcompletegenomesequenceofiacinetobacteriibaumanniiistrainatcc19606revealsnovelmobilegeneticelementsandnovelprophage AT garrysamyers analysisofcompletegenomesequenceofiacinetobacteriibaumanniiistrainatcc19606revealsnovelmobilegeneticelementsandnovelprophage |