Characterization of <i>Anopheles stephensi</i> Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory Transcriptome
Several mosquito species within the genus <i>Anopheles</i> are vectors for human malaria, and the spread of this disease is driven by the propensity of certain species to feed preferentially on humans. The study of olfaction in mosquitoes is important to understand dynamics of host-seeki...
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MDPI AG
2021-06-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/7/593 |
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| author | Zachary Speth Gurlaz Kaur Devin Mazolewski Rayden Sisomphou Danielle Denise C. Siao Rana Pooraiiouby Hans Vasquez-Gross Juli Petereit Monika Gulia-Nuss Dennis Mathew Andrew B. Nuss |
| author_facet | Zachary Speth Gurlaz Kaur Devin Mazolewski Rayden Sisomphou Danielle Denise C. Siao Rana Pooraiiouby Hans Vasquez-Gross Juli Petereit Monika Gulia-Nuss Dennis Mathew Andrew B. Nuss |
| author_sort | Zachary Speth |
| collection | DOAJ |
| description | Several mosquito species within the genus <i>Anopheles</i> are vectors for human malaria, and the spread of this disease is driven by the propensity of certain species to feed preferentially on humans. The study of olfaction in mosquitoes is important to understand dynamics of host-seeking and host-selection; however, the majority of these studies focus on <i>Anopheles gambiae</i> or <i>An. coluzzii</i>, both vectors of malaria in Sub-Saharan Africa. Other malaria vectors may recognize different chemical cues from potential hosts; therefore, in this study, we investigated <i>An. stephensi</i>, the south Asian malaria mosquito. We specifically focused on the mouthparts (primarily the maxillary palp and labella) that have been much less investigated compared to the antennae but are also important for host-seeking. To provide a broad view of chemoreceptor expression, RNAseq was used to examine the transcriptomes from the mouthparts of host-seeking females, blood-fed females, and males. Notably, AsOr8 had a high transcript abundance in all transcriptomes and was, therefore, cloned and expressed in the <i>Drosophila</i> empty neuron system. This permitted characterization with a panel of odorants that were selected, in part, for their presence in the human odor profile. The responsiveness of AsOr8 to odorants was highly similar to <i>An. gambiae</i> Or8 (AgOr8), except for sulcatone, which was detected by AsOr8 but not AgOr8. Subtle differences in the receptor sensitivity to specific odorants may provide clues to species- or strain-specific approaches to host-seeking and host selection. Further exploration of the profile of <i>An. stephensi</i> chemosensory proteins may yield a better understanding of how different malaria vectors navigate host-finding and host-choice. |
| first_indexed | 2024-03-09T04:43:06Z |
| format | Article |
| id | doaj.art-84b4e574643041e7b781b1fc7784e60b |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T04:43:06Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-84b4e574643041e7b781b1fc7784e60b2023-12-03T13:18:19ZengMDPI AGInsects2075-44502021-06-0112759310.3390/insects12070593Characterization of <i>Anopheles stephensi</i> Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory TranscriptomeZachary Speth0Gurlaz Kaur1Devin Mazolewski2Rayden Sisomphou3Danielle Denise C. Siao4Rana Pooraiiouby5Hans Vasquez-Gross6Juli Petereit7Monika Gulia-Nuss8Dennis Mathew9Andrew B. Nuss10Cell and Molecular Biology Graduate Program, University of Nevada, Reno, NV 89557, USACell and Molecular Biology Graduate Program, University of Nevada, Reno, NV 89557, USACell and Molecular Biology Graduate Program, University of Nevada, Reno, NV 89557, USADepartment of Agriculture, Veterinary and Rangeland Sciences, University of Nevada, Reno, NV 89557, USADepartment of Agriculture, Veterinary and Rangeland Sciences, University of Nevada, Reno, NV 89557, USADepartment of Agriculture, Veterinary and Rangeland Sciences, University of Nevada, Reno, NV 89557, USANevada Bioinformatics Center, University of Nevada, Reno, NV 89557, USANevada Bioinformatics Center, University of Nevada, Reno, NV 89557, USADepartment of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, USADepartment of Biology, University of Nevada, Reno, NV 89557, USADepartment of Agriculture, Veterinary and Rangeland Sciences, University of Nevada, Reno, NV 89557, USASeveral mosquito species within the genus <i>Anopheles</i> are vectors for human malaria, and the spread of this disease is driven by the propensity of certain species to feed preferentially on humans. The study of olfaction in mosquitoes is important to understand dynamics of host-seeking and host-selection; however, the majority of these studies focus on <i>Anopheles gambiae</i> or <i>An. coluzzii</i>, both vectors of malaria in Sub-Saharan Africa. Other malaria vectors may recognize different chemical cues from potential hosts; therefore, in this study, we investigated <i>An. stephensi</i>, the south Asian malaria mosquito. We specifically focused on the mouthparts (primarily the maxillary palp and labella) that have been much less investigated compared to the antennae but are also important for host-seeking. To provide a broad view of chemoreceptor expression, RNAseq was used to examine the transcriptomes from the mouthparts of host-seeking females, blood-fed females, and males. Notably, AsOr8 had a high transcript abundance in all transcriptomes and was, therefore, cloned and expressed in the <i>Drosophila</i> empty neuron system. This permitted characterization with a panel of odorants that were selected, in part, for their presence in the human odor profile. The responsiveness of AsOr8 to odorants was highly similar to <i>An. gambiae</i> Or8 (AgOr8), except for sulcatone, which was detected by AsOr8 but not AgOr8. Subtle differences in the receptor sensitivity to specific odorants may provide clues to species- or strain-specific approaches to host-seeking and host selection. Further exploration of the profile of <i>An. stephensi</i> chemosensory proteins may yield a better understanding of how different malaria vectors navigate host-finding and host-choice.https://www.mdpi.com/2075-4450/12/7/593odorant receptorstranscriptomemouthpartschemoreceptormaxillary palpilabellum |
| spellingShingle | Zachary Speth Gurlaz Kaur Devin Mazolewski Rayden Sisomphou Danielle Denise C. Siao Rana Pooraiiouby Hans Vasquez-Gross Juli Petereit Monika Gulia-Nuss Dennis Mathew Andrew B. Nuss Characterization of <i>Anopheles stephensi</i> Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory Transcriptome Insects odorant receptors transcriptome mouthparts chemoreceptor maxillary palpi labellum |
| title | Characterization of <i>Anopheles stephensi</i> Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory Transcriptome |
| title_full | Characterization of <i>Anopheles stephensi</i> Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory Transcriptome |
| title_fullStr | Characterization of <i>Anopheles stephensi</i> Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory Transcriptome |
| title_full_unstemmed | Characterization of <i>Anopheles stephensi</i> Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory Transcriptome |
| title_short | Characterization of <i>Anopheles stephensi</i> Odorant Receptor 8, an Abundant Component of the Mouthpart Chemosensory Transcriptome |
| title_sort | characterization of i anopheles stephensi i odorant receptor 8 an abundant component of the mouthpart chemosensory transcriptome |
| topic | odorant receptors transcriptome mouthparts chemoreceptor maxillary palpi labellum |
| url | https://www.mdpi.com/2075-4450/12/7/593 |
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