Development of aminoglycoside and β-lactamase resistance in intestinal microbiota of swine treated with lincomycin, chlorotetracycline and amoxicillin
Lincomycin, chlortetracycline, and amoxicillin are commonly used antimicrobials for growth promotion and infectious disease prophylaxis in swine production. In this study, we investigated the shifts and resistance development among intestinal microbiota in pregnant sows before and after lincomycin,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-11-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00580/full |
| _version_ | 1811326652714582016 |
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| author | Jian eSun Liang eLi Baotao eLiu Jing eXia Xiaoping eLiao Yahong eLiu |
| author_facet | Jian eSun Liang eLi Baotao eLiu Jing eXia Xiaoping eLiao Yahong eLiu |
| author_sort | Jian eSun |
| collection | DOAJ |
| description | Lincomycin, chlortetracycline, and amoxicillin are commonly used antimicrobials for growth promotion and infectious disease prophylaxis in swine production. In this study, we investigated the shifts and resistance development among intestinal microbiota in pregnant sows before and after lincomycin, chlortetracycline, and amoxicillin treatment by using phylogenetic analysis, bacterial enumeration, and PCR. After the antimicrobial treatment, shifts in microbial community, an increased proportion of resistant bacteria, and genes related to antimicrobial resistance as compared to the day before antimicrobial administration (day 0) were observed. Importantly, a positive correlation between antimicrobial resistance gene expression in different categories, especially those encoding aminoglycoside and β-lactamase and antimicrobial resistance, was observed. These findings demonstrate an important role of antimicrobial usage in animals in the development of antimicrobial resistance, and support the notion that prudent use of antimicrobials in swine is needed to reduce the risk of the emergence of multi-drug resistant (MDR) zoonotic pathogens. |
| first_indexed | 2024-04-13T14:53:34Z |
| format | Article |
| id | doaj.art-84b6dd0b8a3a4d40af9aa07da57f17f1 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T14:53:34Z |
| publishDate | 2014-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-84b6dd0b8a3a4d40af9aa07da57f17f12022-12-22T02:42:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2014-11-01510.3389/fmicb.2014.00580112683Development of aminoglycoside and β-lactamase resistance in intestinal microbiota of swine treated with lincomycin, chlorotetracycline and amoxicillinJian eSun0Liang eLi1Baotao eLiu2Jing eXia3Xiaoping eLiao4Yahong eLiu5South China agricultural universitySouth China agricultural universitySouth China agricultural universitySouth China agricultural universitySouth China agricultural universitySouth China agricultural universityLincomycin, chlortetracycline, and amoxicillin are commonly used antimicrobials for growth promotion and infectious disease prophylaxis in swine production. In this study, we investigated the shifts and resistance development among intestinal microbiota in pregnant sows before and after lincomycin, chlortetracycline, and amoxicillin treatment by using phylogenetic analysis, bacterial enumeration, and PCR. After the antimicrobial treatment, shifts in microbial community, an increased proportion of resistant bacteria, and genes related to antimicrobial resistance as compared to the day before antimicrobial administration (day 0) were observed. Importantly, a positive correlation between antimicrobial resistance gene expression in different categories, especially those encoding aminoglycoside and β-lactamase and antimicrobial resistance, was observed. These findings demonstrate an important role of antimicrobial usage in animals in the development of antimicrobial resistance, and support the notion that prudent use of antimicrobials in swine is needed to reduce the risk of the emergence of multi-drug resistant (MDR) zoonotic pathogens.http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00580/fullqPCR16S rRNAculture-independent methodantibiotic-resistant genesBacterial Enumeration |
| spellingShingle | Jian eSun Liang eLi Baotao eLiu Jing eXia Xiaoping eLiao Yahong eLiu Development of aminoglycoside and β-lactamase resistance in intestinal microbiota of swine treated with lincomycin, chlorotetracycline and amoxicillin Frontiers in Microbiology qPCR 16S rRNA culture-independent method antibiotic-resistant genes Bacterial Enumeration |
| title | Development of aminoglycoside and β-lactamase resistance in intestinal microbiota of swine treated with lincomycin, chlorotetracycline and amoxicillin |
| title_full | Development of aminoglycoside and β-lactamase resistance in intestinal microbiota of swine treated with lincomycin, chlorotetracycline and amoxicillin |
| title_fullStr | Development of aminoglycoside and β-lactamase resistance in intestinal microbiota of swine treated with lincomycin, chlorotetracycline and amoxicillin |
| title_full_unstemmed | Development of aminoglycoside and β-lactamase resistance in intestinal microbiota of swine treated with lincomycin, chlorotetracycline and amoxicillin |
| title_short | Development of aminoglycoside and β-lactamase resistance in intestinal microbiota of swine treated with lincomycin, chlorotetracycline and amoxicillin |
| title_sort | development of aminoglycoside and β lactamase resistance in intestinal microbiota of swine treated with lincomycin chlorotetracycline and amoxicillin |
| topic | qPCR 16S rRNA culture-independent method antibiotic-resistant genes Bacterial Enumeration |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00580/full |
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