Selection and identification of thermophilic yeast strains in leachate from the organic waste heap in Phu Luong district, Thai Nguyen province, Vietnam

The study was carried out to isolate and select useful thermophilic yeast strains in the process of organic domestic waste treatment in Phu Luong - Thai Nguyen. Research results from 23 samples of rust have isolated 10 strains of yeast on YPG medium at 40 oC. Among them, 6 strains of yeast were sele...

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Main Authors: Thi Hong Phuong Dang, Do Thi Tuyen
Format: Article
Language:English
Published: EDP Sciences 2023-01-01
Series:E3S Web of Conferences
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/44/e3sconf_apeem2023_01011.pdf
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author Thi Hong Phuong Dang
Do Thi Tuyen
author_facet Thi Hong Phuong Dang
Do Thi Tuyen
author_sort Thi Hong Phuong Dang
collection DOAJ
description The study was carried out to isolate and select useful thermophilic yeast strains in the process of organic domestic waste treatment in Phu Luong - Thai Nguyen. Research results from 23 samples of rust have isolated 10 strains of yeast on YPG medium at 40 oC. Among them, 6 strains of yeast were selected with the ability to grow and develop in a wide temperature range from 20-45 oC. The results of identification combined with morphological, physiological, and biochemical characteristics of yeast strains showed that, out of 6 selected strains, there were 3 strains belonging to the genus Saccharomyces (Saccharomyces sp. TNY13.01, Saccharomyces sp. TNY22.01), Saccharomyces cerevisiae TNY13.09), 2 strains of the genus Candida (Candida sp. TNY23.01, Candida tropicalis TNY23.126) and 1 strain of the genus Papiliotrema (Papiliotrema laurentii TNY23.127). Among them, the identified strain Saccharomyces cerevisiae TNY13.09 has the ability to grow at 45, tolerates a wide pH range of 4.0– 8.5, has a positive catalase reaction, is capable of using a variety of carbon sources, and belongs to class I biosafety group. On that basis, Saccharomyces cerevisiae TNY13.09 has the potential to be further researched and applied as additional microbial inoculants to the organic waste heap.
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spelling doaj.art-84b723a3a96648a2886aace9fdcc0d882023-08-10T13:16:05ZengEDP SciencesE3S Web of Conferences2267-12422023-01-014070101110.1051/e3sconf/202340701011e3sconf_apeem2023_01011Selection and identification of thermophilic yeast strains in leachate from the organic waste heap in Phu Luong district, Thai Nguyen province, VietnamThi Hong Phuong Dang0Do Thi Tuyen1Department of Biotechnology, Joint Vietnam Russia Tropical Science and Technology Research CenterDepartment of Biotechnology, Joint Vietnam Russia Tropical Science and Technology Research CenterThe study was carried out to isolate and select useful thermophilic yeast strains in the process of organic domestic waste treatment in Phu Luong - Thai Nguyen. Research results from 23 samples of rust have isolated 10 strains of yeast on YPG medium at 40 oC. Among them, 6 strains of yeast were selected with the ability to grow and develop in a wide temperature range from 20-45 oC. The results of identification combined with morphological, physiological, and biochemical characteristics of yeast strains showed that, out of 6 selected strains, there were 3 strains belonging to the genus Saccharomyces (Saccharomyces sp. TNY13.01, Saccharomyces sp. TNY22.01), Saccharomyces cerevisiae TNY13.09), 2 strains of the genus Candida (Candida sp. TNY23.01, Candida tropicalis TNY23.126) and 1 strain of the genus Papiliotrema (Papiliotrema laurentii TNY23.127). Among them, the identified strain Saccharomyces cerevisiae TNY13.09 has the ability to grow at 45, tolerates a wide pH range of 4.0– 8.5, has a positive catalase reaction, is capable of using a variety of carbon sources, and belongs to class I biosafety group. On that basis, Saccharomyces cerevisiae TNY13.09 has the potential to be further researched and applied as additional microbial inoculants to the organic waste heap.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/44/e3sconf_apeem2023_01011.pdf
spellingShingle Thi Hong Phuong Dang
Do Thi Tuyen
Selection and identification of thermophilic yeast strains in leachate from the organic waste heap in Phu Luong district, Thai Nguyen province, Vietnam
E3S Web of Conferences
title Selection and identification of thermophilic yeast strains in leachate from the organic waste heap in Phu Luong district, Thai Nguyen province, Vietnam
title_full Selection and identification of thermophilic yeast strains in leachate from the organic waste heap in Phu Luong district, Thai Nguyen province, Vietnam
title_fullStr Selection and identification of thermophilic yeast strains in leachate from the organic waste heap in Phu Luong district, Thai Nguyen province, Vietnam
title_full_unstemmed Selection and identification of thermophilic yeast strains in leachate from the organic waste heap in Phu Luong district, Thai Nguyen province, Vietnam
title_short Selection and identification of thermophilic yeast strains in leachate from the organic waste heap in Phu Luong district, Thai Nguyen province, Vietnam
title_sort selection and identification of thermophilic yeast strains in leachate from the organic waste heap in phu luong district thai nguyen province vietnam
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/44/e3sconf_apeem2023_01011.pdf
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AT dothituyen selectionandidentificationofthermophilicyeaststrainsinleachatefromtheorganicwasteheapinphuluongdistrictthainguyenprovincevietnam