Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in <it>Vitis vinifera</it>
<p>Abstract</p> <p>The version of this article published in <it>BMC Genomics </it>2009, <b>10:</b>558, contains data in Table <tblr tid="T1">1</tblr> which are now known to be unreliable, and an illustration, in Figure <figr fid=&quo...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-02-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/11/109 |
| _version_ | 1811262850493054976 |
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| author | Policriti Alberto Valle Giorgio Del Fabbro Cristian Casati Cesare Pezzotti Mario Ferrarini Alberto Delledonne Massimo Piccolo Viviana Mica Erica Morgante Michele Pesole Graziano Pè M Enrico Horner David S |
| author_facet | Policriti Alberto Valle Giorgio Del Fabbro Cristian Casati Cesare Pezzotti Mario Ferrarini Alberto Delledonne Massimo Piccolo Viviana Mica Erica Morgante Michele Pesole Graziano Pè M Enrico Horner David S |
| author_sort | Policriti Alberto |
| collection | DOAJ |
| description | <p>Abstract</p> <p>The version of this article published in <it>BMC Genomics </it>2009, <b>10:</b>558, contains data in Table <tblr tid="T1">1</tblr> which are now known to be unreliable, and an illustration, in Figure <figr fid="F1">1</figr>, of unusual miRNA processing events predicted by these unreliable data. In this full-length correction, new data replace those found to be unreliable, leading to a more straightforward interpretation without altering the principle conclusions of the study. Table <tblr tid="T1">1</tblr> and associated methods have been corrected, Figure <figr fid="F1">1</figr> deleted, supplementary file 1 added, and modifications made to the sections "Deep sequencing of small RNAs from grapevine leaf tissue" and "Microarray analysis of miRNA expression". The editors and authors regret the inconvenience caused to readers by premature publication of the original paper.</p> <p>Background</p> <p>MicroRNAs are short (~21 base) single stranded RNAs that, in plants, are generally coded by specific genes and cleaved specifically from hairpin precursors. MicroRNAs are critical for the regulation of multiple developmental, stress related and other physiological processes in plants. The recent annotation of the genome of the grapevine (<it>Vitis vinifera </it>L.) allowed the identification of many putative conserved microRNA precursors, grouped into multiple gene families.</p> <p>Results</p> <p>Here we use oligonucleotide arrays to provide the first indication that many of these microRNAs show differential expression patterns between tissues and during the maturation of fruit in the grapevine. Furthermore we demonstrate that whole transcriptome sequencing and deep-sequencing of small RNA fractions can be used both to identify which microRNA precursors are expressed in different tissues and to estimate genomic coordinates and patterns of splicing and alternative splicing for many primary miRNA transcripts.</p> <p>Conclusions</p> <p>Our results show that many microRNAs are differentially expressed in different tissues and during fruit maturation in the grapevine. Furthermore, the demonstration that whole transcriptome sequencing can be used to identify candidate splicing events and approximate primary microRNA transcript coordinates represents a significant step towards the large-scale elucidation of mechanisms regulating the expression of microRNAs at the transcriptional and post-transcriptional levels.</p> |
| first_indexed | 2024-04-12T19:34:23Z |
| format | Article |
| id | doaj.art-84b8e131612a447aa385d7f19c2c1030 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T19:34:23Z |
| publishDate | 2010-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-84b8e131612a447aa385d7f19c2c10302022-12-22T03:19:15ZengBMCBMC Genomics1471-21642010-02-0111110910.1186/1471-2164-11-109Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in <it>Vitis vinifera</it>Policriti AlbertoValle GiorgioDel Fabbro CristianCasati CesarePezzotti MarioFerrarini AlbertoDelledonne MassimoPiccolo VivianaMica EricaMorgante MichelePesole GrazianoPè M EnricoHorner David S<p>Abstract</p> <p>The version of this article published in <it>BMC Genomics </it>2009, <b>10:</b>558, contains data in Table <tblr tid="T1">1</tblr> which are now known to be unreliable, and an illustration, in Figure <figr fid="F1">1</figr>, of unusual miRNA processing events predicted by these unreliable data. In this full-length correction, new data replace those found to be unreliable, leading to a more straightforward interpretation without altering the principle conclusions of the study. Table <tblr tid="T1">1</tblr> and associated methods have been corrected, Figure <figr fid="F1">1</figr> deleted, supplementary file 1 added, and modifications made to the sections "Deep sequencing of small RNAs from grapevine leaf tissue" and "Microarray analysis of miRNA expression". The editors and authors regret the inconvenience caused to readers by premature publication of the original paper.</p> <p>Background</p> <p>MicroRNAs are short (~21 base) single stranded RNAs that, in plants, are generally coded by specific genes and cleaved specifically from hairpin precursors. MicroRNAs are critical for the regulation of multiple developmental, stress related and other physiological processes in plants. The recent annotation of the genome of the grapevine (<it>Vitis vinifera </it>L.) allowed the identification of many putative conserved microRNA precursors, grouped into multiple gene families.</p> <p>Results</p> <p>Here we use oligonucleotide arrays to provide the first indication that many of these microRNAs show differential expression patterns between tissues and during the maturation of fruit in the grapevine. Furthermore we demonstrate that whole transcriptome sequencing and deep-sequencing of small RNA fractions can be used both to identify which microRNA precursors are expressed in different tissues and to estimate genomic coordinates and patterns of splicing and alternative splicing for many primary miRNA transcripts.</p> <p>Conclusions</p> <p>Our results show that many microRNAs are differentially expressed in different tissues and during fruit maturation in the grapevine. Furthermore, the demonstration that whole transcriptome sequencing can be used to identify candidate splicing events and approximate primary microRNA transcript coordinates represents a significant step towards the large-scale elucidation of mechanisms regulating the expression of microRNAs at the transcriptional and post-transcriptional levels.</p>http://www.biomedcentral.com/1471-2164/11/109 |
| spellingShingle | Policriti Alberto Valle Giorgio Del Fabbro Cristian Casati Cesare Pezzotti Mario Ferrarini Alberto Delledonne Massimo Piccolo Viviana Mica Erica Morgante Michele Pesole Graziano Pè M Enrico Horner David S Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in <it>Vitis vinifera</it> BMC Genomics |
| title | Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in <it>Vitis vinifera</it> |
| title_full | Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in <it>Vitis vinifera</it> |
| title_fullStr | Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in <it>Vitis vinifera</it> |
| title_full_unstemmed | Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in <it>Vitis vinifera</it> |
| title_short | Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in <it>Vitis vinifera</it> |
| title_sort | correction high throughput approaches reveal splicing of primary microrna transcripts and tissue specific expression of mature micrornas in it vitis vinifera it |
| url | http://www.biomedcentral.com/1471-2164/11/109 |
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