Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells.
The organization of the cytoplasm is regulated by molecular motors which transport organelles and other cargoes along cytoskeleton tracks. Melanophores have pigment organelles or melanosomes that move along microtubules toward their minus and plus end by the action of cytoplasmic dynein and kinesin-...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-04-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3069964?pdf=render |
| _version_ | 1818138595358146560 |
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| author | Luciana Bruno Marcelo Salierno Diana E Wetzler Marcelo A Despósito Valeria Levi |
| author_facet | Luciana Bruno Marcelo Salierno Diana E Wetzler Marcelo A Despósito Valeria Levi |
| author_sort | Luciana Bruno |
| collection | DOAJ |
| description | The organization of the cytoplasm is regulated by molecular motors which transport organelles and other cargoes along cytoskeleton tracks. Melanophores have pigment organelles or melanosomes that move along microtubules toward their minus and plus end by the action of cytoplasmic dynein and kinesin-2, respectively. In this work, we used single particle tracking to characterize the mechanical properties of motor-driven organelles during transport along microtubules. We tracked organelles with high temporal and spatial resolutions and characterized their dynamics perpendicular to the cytoskeleton track. The quantitative analysis of these data showed that the dynamics is due to a spring-like interaction between melanosomes and microtubules in a viscoelastic microenvironment. A model based on a generalized Langevin equation explained these observations and predicted that the stiffness measured for the motor complex acting as a linker between organelles and microtubules is ∼ one order smaller than that determined for motor proteins in vitro. This result suggests that other biomolecules involved in the interaction between motors and organelles contribute to the mechanical properties of the motor complex. We hypothesise that the high flexibility observed for the motor linker may be required to improve the efficiency of the transport driven by multiple copies of motor molecules. |
| first_indexed | 2024-12-11T10:14:42Z |
| format | Article |
| id | doaj.art-84b9ef0c176d44c48db04d67b4b31168 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T10:14:42Z |
| publishDate | 2011-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-84b9ef0c176d44c48db04d67b4b311682022-12-22T01:11:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-04-0164e1833210.1371/journal.pone.0018332Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells.Luciana BrunoMarcelo SaliernoDiana E WetzlerMarcelo A DespósitoValeria LeviThe organization of the cytoplasm is regulated by molecular motors which transport organelles and other cargoes along cytoskeleton tracks. Melanophores have pigment organelles or melanosomes that move along microtubules toward their minus and plus end by the action of cytoplasmic dynein and kinesin-2, respectively. In this work, we used single particle tracking to characterize the mechanical properties of motor-driven organelles during transport along microtubules. We tracked organelles with high temporal and spatial resolutions and characterized their dynamics perpendicular to the cytoskeleton track. The quantitative analysis of these data showed that the dynamics is due to a spring-like interaction between melanosomes and microtubules in a viscoelastic microenvironment. A model based on a generalized Langevin equation explained these observations and predicted that the stiffness measured for the motor complex acting as a linker between organelles and microtubules is ∼ one order smaller than that determined for motor proteins in vitro. This result suggests that other biomolecules involved in the interaction between motors and organelles contribute to the mechanical properties of the motor complex. We hypothesise that the high flexibility observed for the motor linker may be required to improve the efficiency of the transport driven by multiple copies of motor molecules.http://europepmc.org/articles/PMC3069964?pdf=render |
| spellingShingle | Luciana Bruno Marcelo Salierno Diana E Wetzler Marcelo A Despósito Valeria Levi Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells. PLoS ONE |
| title | Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells. |
| title_full | Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells. |
| title_fullStr | Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells. |
| title_full_unstemmed | Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells. |
| title_short | Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells. |
| title_sort | mechanical properties of organelles driven by microtubule dependent molecular motors in living cells |
| url | http://europepmc.org/articles/PMC3069964?pdf=render |
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