Electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic SOD, catalase in an in vitro assay system by pectic polysaccharides of Momordica charantia in combination with curcumin
Momordica charantia (MC) fruit known as bitter gourd, is of potential nutritional and medicinal value. The objectives of the present in vitro study were to evaluate the efficacy of bioactive pectic polysaccharides (CCPS) of MC along with another well-known bioactive compound curcumin in the abrogati...
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| Format: | Article |
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Elsevier
2017-09-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580816303284 |
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| author | Hasina Perveen Moumita Dash Shamima Khatun Moulima Maity Syed Sirajul Islam Sandip Chattopadhyay |
| author_facet | Hasina Perveen Moumita Dash Shamima Khatun Moulima Maity Syed Sirajul Islam Sandip Chattopadhyay |
| author_sort | Hasina Perveen |
| collection | DOAJ |
| description | Momordica charantia (MC) fruit known as bitter gourd, is of potential nutritional and medicinal value. The objectives of the present in vitro study were to evaluate the efficacy of bioactive pectic polysaccharides (CCPS) of MC along with another well-known bioactive compound curcumin in the abrogation of hepatocellular oxidative stress persuaded by sodium arsenite. Electrozymographic method was developed for the assessment of superoxide dismutase (SOD) and catalase activities of liver tissues maintained under an in vitro system. A significant association of CCPS of MC in combination with curcumin was found in the alleviation of oxidative stress induced by sodium arsenite in liver slice. Generated data pointed out that CCPS of MC and curcumin separately or in combination can offer significant protection against alterations in malondialdehyde (MDA), conjugated diene (CD) and antioxidative defense (SOD, CAT) markers. Furthermore, results of hepatic cell DNA degradation strongly supported that both these co-administrations have efficacy in preventing cellular damage. This is the first information of extracted polysaccharides from MC preventing arsenic induced damage in a liver slice of rat. |
| first_indexed | 2024-04-12T08:16:49Z |
| format | Article |
| id | doaj.art-84be19ae4eea4e458eb8a72dadecab1a |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-04-12T08:16:49Z |
| publishDate | 2017-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-84be19ae4eea4e458eb8a72dadecab1a2022-12-22T03:40:47ZengElsevierBiochemistry and Biophysics Reports2405-58082017-09-0111C647110.1016/j.bbrep.2017.06.002Electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic SOD, catalase in an in vitro assay system by pectic polysaccharides of Momordica charantia in combination with curcuminHasina Perveen0Moumita Dash1Shamima Khatun2Moulima Maity3Syed Sirajul Islam4Sandip Chattopadhyay5Department of Biomedical Laboratory Science and Management, and Clinical Nutrition and Dietetics division, (UGC Innovative Department), Vidyasagar University, Midnapore, West Bengal 721102, IndiaDepartment of Biomedical Laboratory Science and Management, and Clinical Nutrition and Dietetics division, (UGC Innovative Department), Vidyasagar University, Midnapore, West Bengal 721102, IndiaDepartment of Biomedical Laboratory Science and Management, and Clinical Nutrition and Dietetics division, (UGC Innovative Department), Vidyasagar University, Midnapore, West Bengal 721102, IndiaDepartment of Biomedical Laboratory Science and Management, and Clinical Nutrition and Dietetics division, (UGC Innovative Department), Vidyasagar University, Midnapore, West Bengal 721102, IndiaDepartment of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, IndiaDepartment of Biomedical Laboratory Science and Management, and Clinical Nutrition and Dietetics division, (UGC Innovative Department), Vidyasagar University, Midnapore, West Bengal 721102, IndiaMomordica charantia (MC) fruit known as bitter gourd, is of potential nutritional and medicinal value. The objectives of the present in vitro study were to evaluate the efficacy of bioactive pectic polysaccharides (CCPS) of MC along with another well-known bioactive compound curcumin in the abrogation of hepatocellular oxidative stress persuaded by sodium arsenite. Electrozymographic method was developed for the assessment of superoxide dismutase (SOD) and catalase activities of liver tissues maintained under an in vitro system. A significant association of CCPS of MC in combination with curcumin was found in the alleviation of oxidative stress induced by sodium arsenite in liver slice. Generated data pointed out that CCPS of MC and curcumin separately or in combination can offer significant protection against alterations in malondialdehyde (MDA), conjugated diene (CD) and antioxidative defense (SOD, CAT) markers. Furthermore, results of hepatic cell DNA degradation strongly supported that both these co-administrations have efficacy in preventing cellular damage. This is the first information of extracted polysaccharides from MC preventing arsenic induced damage in a liver slice of rat.http://www.sciencedirect.com/science/article/pii/S2405580816303284ArsenicCCPSCurcuminOxidative stressDNA |
| spellingShingle | Hasina Perveen Moumita Dash Shamima Khatun Moulima Maity Syed Sirajul Islam Sandip Chattopadhyay Electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic SOD, catalase in an in vitro assay system by pectic polysaccharides of Momordica charantia in combination with curcumin Biochemistry and Biophysics Reports Arsenic CCPS Curcumin Oxidative stress DNA |
| title | Electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic SOD, catalase in an in vitro assay system by pectic polysaccharides of Momordica charantia in combination with curcumin |
| title_full | Electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic SOD, catalase in an in vitro assay system by pectic polysaccharides of Momordica charantia in combination with curcumin |
| title_fullStr | Electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic SOD, catalase in an in vitro assay system by pectic polysaccharides of Momordica charantia in combination with curcumin |
| title_full_unstemmed | Electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic SOD, catalase in an in vitro assay system by pectic polysaccharides of Momordica charantia in combination with curcumin |
| title_short | Electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic SOD, catalase in an in vitro assay system by pectic polysaccharides of Momordica charantia in combination with curcumin |
| title_sort | electrozymographic evaluation of the attenuation of arsenic induced degradation of hepatic sod catalase in an in vitro assay system by pectic polysaccharides of momordica charantia in combination with curcumin |
| topic | Arsenic CCPS Curcumin Oxidative stress DNA |
| url | http://www.sciencedirect.com/science/article/pii/S2405580816303284 |
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