Kinesin-1 activity recorded in living cells with a precipitating dye
Monitoring the activity of the processive motor protein kinesin-1 in live cells is currently difficult. Here the authors report the fluorogenic small molecule QPD-OTf, a kinesin-1 substrate that causes activity-dependent dye precipitation.
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-21626-1 |
| _version_ | 1819202174552375296 |
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| author | Simona Angerani Eric Lindberg Nikolai Klena Christopher K. E. Bleck Charlotte Aumeier Nicolas Winssinger |
| author_facet | Simona Angerani Eric Lindberg Nikolai Klena Christopher K. E. Bleck Charlotte Aumeier Nicolas Winssinger |
| author_sort | Simona Angerani |
| collection | DOAJ |
| description | Monitoring the activity of the processive motor protein kinesin-1 in live cells is currently difficult. Here the authors report the fluorogenic small molecule QPD-OTf, a kinesin-1 substrate that causes activity-dependent dye precipitation. |
| first_indexed | 2024-12-23T03:59:50Z |
| format | Article |
| id | doaj.art-84c103ff473b4a30af353c47c941bdf3 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-23T03:59:50Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-84c103ff473b4a30af353c47c941bdf32022-12-21T18:00:45ZengNature PortfolioNature Communications2041-17232021-03-0112111010.1038/s41467-021-21626-1Kinesin-1 activity recorded in living cells with a precipitating dyeSimona Angerani0Eric Lindberg1Nikolai Klena2Christopher K. E. Bleck3Charlotte Aumeier4Nicolas Winssinger5School of Chemistry and Biochemistry, NCCR Chemical Biology, Faculty of Science, University of GenevaSchool of Chemistry and Biochemistry, NCCR Chemical Biology, Faculty of Science, University of GenevaDepartment of Cell Biology, Faculty of Science, University of GenevaElectron Microscopy Core Facility, National Heart, Lung and Blood Institute, National Institutes of HealthSchool of Chemistry and Biochemistry, NCCR Chemical Biology, Faculty of Science, University of GenevaSchool of Chemistry and Biochemistry, NCCR Chemical Biology, Faculty of Science, University of GenevaMonitoring the activity of the processive motor protein kinesin-1 in live cells is currently difficult. Here the authors report the fluorogenic small molecule QPD-OTf, a kinesin-1 substrate that causes activity-dependent dye precipitation.https://doi.org/10.1038/s41467-021-21626-1 |
| spellingShingle | Simona Angerani Eric Lindberg Nikolai Klena Christopher K. E. Bleck Charlotte Aumeier Nicolas Winssinger Kinesin-1 activity recorded in living cells with a precipitating dye Nature Communications |
| title | Kinesin-1 activity recorded in living cells with a precipitating dye |
| title_full | Kinesin-1 activity recorded in living cells with a precipitating dye |
| title_fullStr | Kinesin-1 activity recorded in living cells with a precipitating dye |
| title_full_unstemmed | Kinesin-1 activity recorded in living cells with a precipitating dye |
| title_short | Kinesin-1 activity recorded in living cells with a precipitating dye |
| title_sort | kinesin 1 activity recorded in living cells with a precipitating dye |
| url | https://doi.org/10.1038/s41467-021-21626-1 |
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