Spectroscopic Signature of Red Blood Cells in a D-Galactose-Induced Accelerated Aging Model
This work presents a semi-quantitative spectroscopic approach, including FTIR–ATR and Raman spectroscopies, for the biochemical analysis of red blood cells (RBCs) supported by the biochemical, morphological and rheological reference techniques. This multi-modal approach provided the description of t...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/5/2660 |
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| author | Aneta Blat Tetiana Stepanenko Katarzyna Bulat Aleksandra Wajda Jakub Dybas Tasnim Mohaissen Fatih Celal Alcicek Ewa Szczesny-Malysiak Kamilla Malek Andrzej Fedorowicz Katarzyna M. Marzec |
| author_facet | Aneta Blat Tetiana Stepanenko Katarzyna Bulat Aleksandra Wajda Jakub Dybas Tasnim Mohaissen Fatih Celal Alcicek Ewa Szczesny-Malysiak Kamilla Malek Andrzej Fedorowicz Katarzyna M. Marzec |
| author_sort | Aneta Blat |
| collection | DOAJ |
| description | This work presents a semi-quantitative spectroscopic approach, including FTIR–ATR and Raman spectroscopies, for the biochemical analysis of red blood cells (RBCs) supported by the biochemical, morphological and rheological reference techniques. This multi-modal approach provided the description of the RBC alterations at the molecular level in a model of accelerated aging induced by administration of D-galactose (D-gal), in comparison to natural aging. Such an approach allowed to conclude that most age-related biochemical RBC membrane changes (a decrease in lipid unsaturation and the level of phospholipids, or an increase in acyl chain shortening) as well as alterations in the morphological parameters and RBC deformability are well reflected in the D-gal model of accelerated aging. Similarly, as in natural aging, a decrease in LDL level in blood plasma and no changes in the fraction of glucose, creatinine, total cholesterol, HDL, iron, or triglycerides were observed during the course of accelerated aging. Contrary to natural aging, the D-gal model led to an increase in cholesterol esters and the fraction of total esterified lipids in RBC membranes, and evoked significant changes in the secondary structure of the membrane proteins. Moreover, a significant decrease in the phosphorous level of blood plasma was specific for the D-gal model. On the other hand, natural aging induced stronger changes in the secondary structures of the proteins of the RBCs’ interior. This work proves that research on the aging mechanism, especially in circulation-related diseases, should employ the D-gal model with caution. Nonetheless, the D-gal model enables to imitate age-related rheological alterations in RBCs, although they are partially derived from different changes observed in the RBC membrane at the molecular level. |
| first_indexed | 2024-03-09T05:12:03Z |
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| id | doaj.art-84c5070d1a1d4b89a9601c91f6b7f705 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:12:03Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-84c5070d1a1d4b89a9601c91f6b7f7052023-12-03T12:48:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01225266010.3390/ijms22052660Spectroscopic Signature of Red Blood Cells in a D-Galactose-Induced Accelerated Aging ModelAneta Blat0Tetiana Stepanenko1Katarzyna Bulat2Aleksandra Wajda3Jakub Dybas4Tasnim Mohaissen5Fatih Celal Alcicek6Ewa Szczesny-Malysiak7Kamilla Malek8Andrzej Fedorowicz9Katarzyna M. Marzec10Jagiellonian Center for Experimental Therapeutics, Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, PolandJagiellonian Center for Experimental Therapeutics, Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, PolandJagiellonian Center for Experimental Therapeutics, Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, PolandJagiellonian Center for Experimental Therapeutics, Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, PolandJagiellonian Center for Experimental Therapeutics, Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, PolandJagiellonian Center for Experimental Therapeutics, Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, PolandJagiellonian Center for Experimental Therapeutics, Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, PolandJagiellonian Center for Experimental Therapeutics, Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, PolandFaculty of Chemistry, Jagiellonian University, 2 Gronostajowa Str., 30-387 Krakow, PolandChair of Pharmacology, Jagiellonian University Medical College, 16 Grzegorzecka Str., 31-531 Krakow, PolandJagiellonian Center for Experimental Therapeutics, Jagiellonian University, 14 Bobrzynskiego Str., 30-348 Krakow, PolandThis work presents a semi-quantitative spectroscopic approach, including FTIR–ATR and Raman spectroscopies, for the biochemical analysis of red blood cells (RBCs) supported by the biochemical, morphological and rheological reference techniques. This multi-modal approach provided the description of the RBC alterations at the molecular level in a model of accelerated aging induced by administration of D-galactose (D-gal), in comparison to natural aging. Such an approach allowed to conclude that most age-related biochemical RBC membrane changes (a decrease in lipid unsaturation and the level of phospholipids, or an increase in acyl chain shortening) as well as alterations in the morphological parameters and RBC deformability are well reflected in the D-gal model of accelerated aging. Similarly, as in natural aging, a decrease in LDL level in blood plasma and no changes in the fraction of glucose, creatinine, total cholesterol, HDL, iron, or triglycerides were observed during the course of accelerated aging. Contrary to natural aging, the D-gal model led to an increase in cholesterol esters and the fraction of total esterified lipids in RBC membranes, and evoked significant changes in the secondary structure of the membrane proteins. Moreover, a significant decrease in the phosphorous level of blood plasma was specific for the D-gal model. On the other hand, natural aging induced stronger changes in the secondary structures of the proteins of the RBCs’ interior. This work proves that research on the aging mechanism, especially in circulation-related diseases, should employ the D-gal model with caution. Nonetheless, the D-gal model enables to imitate age-related rheological alterations in RBCs, although they are partially derived from different changes observed in the RBC membrane at the molecular level.https://www.mdpi.com/1422-0067/22/5/2660red blood cells (RBCs)agingRBC membranesD-galactose-induced accelerated aging mouse modelvibrational spectroscopyRaman spectroscopy |
| spellingShingle | Aneta Blat Tetiana Stepanenko Katarzyna Bulat Aleksandra Wajda Jakub Dybas Tasnim Mohaissen Fatih Celal Alcicek Ewa Szczesny-Malysiak Kamilla Malek Andrzej Fedorowicz Katarzyna M. Marzec Spectroscopic Signature of Red Blood Cells in a D-Galactose-Induced Accelerated Aging Model International Journal of Molecular Sciences red blood cells (RBCs) aging RBC membranes D-galactose-induced accelerated aging mouse model vibrational spectroscopy Raman spectroscopy |
| title | Spectroscopic Signature of Red Blood Cells in a D-Galactose-Induced Accelerated Aging Model |
| title_full | Spectroscopic Signature of Red Blood Cells in a D-Galactose-Induced Accelerated Aging Model |
| title_fullStr | Spectroscopic Signature of Red Blood Cells in a D-Galactose-Induced Accelerated Aging Model |
| title_full_unstemmed | Spectroscopic Signature of Red Blood Cells in a D-Galactose-Induced Accelerated Aging Model |
| title_short | Spectroscopic Signature of Red Blood Cells in a D-Galactose-Induced Accelerated Aging Model |
| title_sort | spectroscopic signature of red blood cells in a d galactose induced accelerated aging model |
| topic | red blood cells (RBCs) aging RBC membranes D-galactose-induced accelerated aging mouse model vibrational spectroscopy Raman spectroscopy |
| url | https://www.mdpi.com/1422-0067/22/5/2660 |
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