Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks
In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and m...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2010-01-01
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| Series: | Biomarker Insights |
| Online Access: | https://doi.org/10.4137/BMI.S5522 |
| _version_ | 1818242983515914240 |
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| author | Daniel Gilbert Weber Swaantje Casjens Peter Rozynek Martin Lehnert Sandra Zilch-Schöneweis Oleksandr Bryk Dirk Taeger Maria Gomolka Michaela Kreuzer Heinz Otten Beate Pesch Georg Johnen Thomas Brüning |
| author_facet | Daniel Gilbert Weber Swaantje Casjens Peter Rozynek Martin Lehnert Sandra Zilch-Schöneweis Oleksandr Bryk Dirk Taeger Maria Gomolka Michaela Kreuzer Heinz Otten Beate Pesch Georg Johnen Thomas Brüning |
| author_sort | Daniel Gilbert Weber |
| collection | DOAJ |
| description | In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies. |
| first_indexed | 2024-12-12T13:53:54Z |
| format | Article |
| id | doaj.art-84c7549e7f6c43189e89099e3c17f180 |
| institution | Directory Open Access Journal |
| issn | 1177-2719 |
| language | English |
| last_indexed | 2024-12-12T13:53:54Z |
| publishDate | 2010-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Biomarker Insights |
| spelling | doaj.art-84c7549e7f6c43189e89099e3c17f1802022-12-22T00:22:31ZengSAGE PublishingBiomarker Insights1177-27192010-01-01510.4137/BMI.S5522Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and BiobanksDaniel Gilbert Weber0Swaantje Casjens1Peter Rozynek2Martin Lehnert3Sandra Zilch-Schöneweis4Oleksandr Bryk5Dirk Taeger6Maria Gomolka7Michaela Kreuzer8Heinz Otten9Beate Pesch10Georg Johnen11Thomas Brüning12Institute for Prevention and Occupational Medicine of the German Social Accident Insurance–-Institute of the Ruhr-Universität Bochum (IPA), Bochum, Germany.Institute for Prevention and Occupational Medicine of the German Social Accident Insurance–-Institute of the Ruhr-Universität Bochum (IPA), Bochum, Germany.Institute for Prevention and Occupational Medicine of the German Social Accident Insurance–-Institute of the Ruhr-Universität Bochum (IPA), Bochum, Germany.Institute for Prevention and Occupational Medicine of the German Social Accident Insurance–-Institute of the Ruhr-Universität Bochum (IPA), Bochum, Germany.Institute for Prevention and Occupational Medicine of the German Social Accident Insurance–-Institute of the Ruhr-Universität Bochum (IPA), Bochum, Germany.Institute for Prevention and Occupational Medicine of the German Social Accident Insurance–-Institute of the Ruhr-Universität Bochum (IPA), Bochum, Germany.Institute for Prevention and Occupational Medicine of the German Social Accident Insurance–-Institute of the Ruhr-Universität Bochum (IPA), Bochum, Germany.Department of Radiation Protection and Health, Federal Office for Radiation Protection, Oberschleissheim, Germany.Department of Radiation Protection and Health, Federal Office for Radiation Protection, Oberschleissheim, Germany.German Social Accident Insurance (DGUV), Sankt Augustin, Germany.Institute for Prevention and Occupational Medicine of the German Social Accident Insurance–-Institute of the Ruhr-Universität Bochum (IPA), Bochum, Germany.Institute for Prevention and Occupational Medicine of the German Social Accident Insurance–-Institute of the Ruhr-Universität Bochum (IPA), Bochum, Germany.Institute for Prevention and Occupational Medicine of the German Social Accident Insurance–-Institute of the Ruhr-Universität Bochum (IPA), Bochum, Germany.In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies.https://doi.org/10.4137/BMI.S5522 |
| spellingShingle | Daniel Gilbert Weber Swaantje Casjens Peter Rozynek Martin Lehnert Sandra Zilch-Schöneweis Oleksandr Bryk Dirk Taeger Maria Gomolka Michaela Kreuzer Heinz Otten Beate Pesch Georg Johnen Thomas Brüning Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks Biomarker Insights |
| title | Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks |
| title_full | Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks |
| title_fullStr | Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks |
| title_full_unstemmed | Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks |
| title_short | Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks |
| title_sort | assessment of mrna and microrna stabilization in peripheral human blood for multicenter studies and biobanks |
| url | https://doi.org/10.4137/BMI.S5522 |
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