Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human Plasma
Exosomes that are released by T cells are key messengers involved in immune regulation. However, the molecular profiling of these vesicles, which is necessary for understanding their functions, requires their isolation from a very heterogeneous mixture of extracellular vesicles that are present in t...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/12/1965 |
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| author | Aneta Zebrowska Karol Jelonek Sujan Mondal Marta Gawin Katarzyna Mrowiec Piotr Widłak Theresa Whiteside Monika Pietrowska |
| author_facet | Aneta Zebrowska Karol Jelonek Sujan Mondal Marta Gawin Katarzyna Mrowiec Piotr Widłak Theresa Whiteside Monika Pietrowska |
| author_sort | Aneta Zebrowska |
| collection | DOAJ |
| description | Exosomes that are released by T cells are key messengers involved in immune regulation. However, the molecular profiling of these vesicles, which is necessary for understanding their functions, requires their isolation from a very heterogeneous mixture of extracellular vesicles that are present in the human plasma. It has been shown that exosomes that are produced by T cells could be isolated from plasma by immune capture using antibodies that target the CD3 antigen, which is a key component of the TCR complex that is present in all T lymphocytes. Here, we demonstrate that CD3(+) exosomes that are isolated from plasma can be used for high-throughput molecular profiling using proteomics and metabolomics tools. This profiling allowed for the identification of proteins and metabolites that differentiated the CD3(+) from the CD3(−) exosome fractions that were present in the plasma of healthy donors. Importantly, the proteins and metabolites that accumulated in the CD3(+) vesicles reflected the known molecular features of T lymphocytes. Hence, CD3(+) exosomes that are isolated from human plasma by immune capture could serve as a “T cell biopsy”. |
| first_indexed | 2024-03-10T00:09:17Z |
| format | Article |
| id | doaj.art-84c7654006ca433d810b62e977494c96 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:09:17Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-84c7654006ca433d810b62e977494c962023-11-23T16:02:17ZengMDPI AGCells2073-44092022-06-011112196510.3390/cells11121965Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human PlasmaAneta Zebrowska0Karol Jelonek1Sujan Mondal2Marta Gawin3Katarzyna Mrowiec4Piotr Widłak5Theresa Whiteside6Monika Pietrowska7Maria Sklodowska-Curie National Research Institute of Oncology, 44-102 Gliwice, PolandMaria Sklodowska-Curie National Research Institute of Oncology, 44-102 Gliwice, PolandUPMC Hillman Cancer Center, University of Pittsburgh Cancer Institute, Pittsburgh, PA 15232, USAMaria Sklodowska-Curie National Research Institute of Oncology, 44-102 Gliwice, PolandMaria Sklodowska-Curie National Research Institute of Oncology, 44-102 Gliwice, PolandClinical Research Support Centre, Medical University of Gdańsk, 80-210 Gdańsk, PolandUPMC Hillman Cancer Center, University of Pittsburgh Cancer Institute, Pittsburgh, PA 15232, USAMaria Sklodowska-Curie National Research Institute of Oncology, 44-102 Gliwice, PolandExosomes that are released by T cells are key messengers involved in immune regulation. However, the molecular profiling of these vesicles, which is necessary for understanding their functions, requires their isolation from a very heterogeneous mixture of extracellular vesicles that are present in the human plasma. It has been shown that exosomes that are produced by T cells could be isolated from plasma by immune capture using antibodies that target the CD3 antigen, which is a key component of the TCR complex that is present in all T lymphocytes. Here, we demonstrate that CD3(+) exosomes that are isolated from plasma can be used for high-throughput molecular profiling using proteomics and metabolomics tools. This profiling allowed for the identification of proteins and metabolites that differentiated the CD3(+) from the CD3(−) exosome fractions that were present in the plasma of healthy donors. Importantly, the proteins and metabolites that accumulated in the CD3(+) vesicles reflected the known molecular features of T lymphocytes. Hence, CD3(+) exosomes that are isolated from human plasma by immune capture could serve as a “T cell biopsy”.https://www.mdpi.com/2073-4409/11/12/1965CD3 antigenexosomesimmune captureT lymphocytesmetabolomicsproteomics |
| spellingShingle | Aneta Zebrowska Karol Jelonek Sujan Mondal Marta Gawin Katarzyna Mrowiec Piotr Widłak Theresa Whiteside Monika Pietrowska Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human Plasma Cells CD3 antigen exosomes immune capture T lymphocytes metabolomics proteomics |
| title | Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human Plasma |
| title_full | Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human Plasma |
| title_fullStr | Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human Plasma |
| title_full_unstemmed | Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human Plasma |
| title_short | Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human Plasma |
| title_sort | proteomic and metabolomic profiles of t cell derived exosomes isolated from human plasma |
| topic | CD3 antigen exosomes immune capture T lymphocytes metabolomics proteomics |
| url | https://www.mdpi.com/2073-4409/11/12/1965 |
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