Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study
Abstract Background Peri-implantitis therapy is a major problem in implantology. Because of challenging rough implant surface and implant geometry, microorganisms can hide and survive in implant microstructures and impede debridement. We developed a new water jet (WJ) device and a new cold atmospher...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-05-01
|
| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-022-02195-1 |
| _version_ | 1828320699564949504 |
|---|---|
| author | Rutger Matthes Lukasz Jablonowski Vinay Pitchika Birte Holtfreter Christian Eberhard Leo Seifert Torsten Gerling Laura Vilardell Scholten Rabea Schlüter Thomas Kocher |
| author_facet | Rutger Matthes Lukasz Jablonowski Vinay Pitchika Birte Holtfreter Christian Eberhard Leo Seifert Torsten Gerling Laura Vilardell Scholten Rabea Schlüter Thomas Kocher |
| author_sort | Rutger Matthes |
| collection | DOAJ |
| description | Abstract Background Peri-implantitis therapy is a major problem in implantology. Because of challenging rough implant surface and implant geometry, microorganisms can hide and survive in implant microstructures and impede debridement. We developed a new water jet (WJ) device and a new cold atmospheric pressure plasma (CAP) device to overcome these problems and investigated aspects of efficacy in vitro and safety with the aim to create the prerequisites for a clinical pilot study with these medical devices. Methods We compared the efficiency of a single treatment with a WJ or curette and cotton swab (CC) without or with adjunctive use of CAP (WJ + CAP, CC + CAP) to remove biofilm in vitro from rough titanium discs. Treatment efficacy was evaluated by measuring turbidity up to 72 h for bacterial re-growth or spreading of osteoblast-like cells (MG-63) after 5 days with scanning electron microscopy. With respect to application safety, the WJ and CAP instruments were examined according to basic regulations for medical devices. Results After 96 h of incubation all WJ and CC treated disks were turbid but 67% of WJ + CAP and 46% CC + CAP treated specimens were still clear. The increase in turbidity after WJ treatment was delayed by about 20 h compared to CC treatment. In combination with CAP the cell coverage significantly increased to 82% (WJ + CAP) or 72% (CC + CAP), compared to single treatment 11% (WJ) or 10% (CC). Conclusion The newly developed water jet device effectively removes biofilm from rough titanium surfaces in vitro and, in combination with the new CAP device, biologically acceptable surfaces allow osteoblasts to grow. WJ in combination with CAP leads to cleaner surfaces than the usage of curette and cotton swabs with or without subsequent plasma treatment. Our next step will be a clinical pilot study with these new devices to assess the clinical healing process. |
| first_indexed | 2024-04-13T18:15:12Z |
| format | Article |
| id | doaj.art-84c767e99d0e40d69f86eba5173e8f82 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-13T18:15:12Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-84c767e99d0e40d69f86eba5173e8f822022-12-22T02:35:42ZengBMCBMC Oral Health1472-68312022-05-0122111610.1186/s12903-022-02195-1Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro studyRutger Matthes0Lukasz Jablonowski1Vinay Pitchika2Birte Holtfreter3Christian Eberhard4Leo Seifert5Torsten Gerling6Laura Vilardell Scholten7Rabea Schlüter8Thomas Kocher9Department of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine GreifswaldDepartment of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine GreifswaldDepartment of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine GreifswaldDepartment of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine GreifswaldSirona Dental Systems GmbHSirona Dental Systems GmbHZIK Plasmatis, Leibniz-Institute for Plasma Science and Technology e.V. (INP)ZIK Plasmatis, Leibniz-Institute for Plasma Science and Technology e.V. (INP)Imaging Center of the Department of Biology, University of GreifswaldDepartment of Restorative Dentistry, Periodontology, Endodontology, Preventive Dentistry and Pedodontics, Dental School, University Medicine GreifswaldAbstract Background Peri-implantitis therapy is a major problem in implantology. Because of challenging rough implant surface and implant geometry, microorganisms can hide and survive in implant microstructures and impede debridement. We developed a new water jet (WJ) device and a new cold atmospheric pressure plasma (CAP) device to overcome these problems and investigated aspects of efficacy in vitro and safety with the aim to create the prerequisites for a clinical pilot study with these medical devices. Methods We compared the efficiency of a single treatment with a WJ or curette and cotton swab (CC) without or with adjunctive use of CAP (WJ + CAP, CC + CAP) to remove biofilm in vitro from rough titanium discs. Treatment efficacy was evaluated by measuring turbidity up to 72 h for bacterial re-growth or spreading of osteoblast-like cells (MG-63) after 5 days with scanning electron microscopy. With respect to application safety, the WJ and CAP instruments were examined according to basic regulations for medical devices. Results After 96 h of incubation all WJ and CC treated disks were turbid but 67% of WJ + CAP and 46% CC + CAP treated specimens were still clear. The increase in turbidity after WJ treatment was delayed by about 20 h compared to CC treatment. In combination with CAP the cell coverage significantly increased to 82% (WJ + CAP) or 72% (CC + CAP), compared to single treatment 11% (WJ) or 10% (CC). Conclusion The newly developed water jet device effectively removes biofilm from rough titanium surfaces in vitro and, in combination with the new CAP device, biologically acceptable surfaces allow osteoblasts to grow. WJ in combination with CAP leads to cleaner surfaces than the usage of curette and cotton swabs with or without subsequent plasma treatment. Our next step will be a clinical pilot study with these new devices to assess the clinical healing process.https://doi.org/10.1186/s12903-022-02195-1BiofilmWater jetCold plasmaTitanium surfacePeri-implantitis |
| spellingShingle | Rutger Matthes Lukasz Jablonowski Vinay Pitchika Birte Holtfreter Christian Eberhard Leo Seifert Torsten Gerling Laura Vilardell Scholten Rabea Schlüter Thomas Kocher Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study BMC Oral Health Biofilm Water jet Cold plasma Titanium surface Peri-implantitis |
| title | Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study |
| title_full | Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study |
| title_fullStr | Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study |
| title_full_unstemmed | Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study |
| title_short | Efficiency of biofilm removal by combination of water jet and cold plasma: an in-vitro study |
| title_sort | efficiency of biofilm removal by combination of water jet and cold plasma an in vitro study |
| topic | Biofilm Water jet Cold plasma Titanium surface Peri-implantitis |
| url | https://doi.org/10.1186/s12903-022-02195-1 |
| work_keys_str_mv | AT rutgermatthes efficiencyofbiofilmremovalbycombinationofwaterjetandcoldplasmaaninvitrostudy AT lukaszjablonowski efficiencyofbiofilmremovalbycombinationofwaterjetandcoldplasmaaninvitrostudy AT vinaypitchika efficiencyofbiofilmremovalbycombinationofwaterjetandcoldplasmaaninvitrostudy AT birteholtfreter efficiencyofbiofilmremovalbycombinationofwaterjetandcoldplasmaaninvitrostudy AT christianeberhard efficiencyofbiofilmremovalbycombinationofwaterjetandcoldplasmaaninvitrostudy AT leoseifert efficiencyofbiofilmremovalbycombinationofwaterjetandcoldplasmaaninvitrostudy AT torstengerling efficiencyofbiofilmremovalbycombinationofwaterjetandcoldplasmaaninvitrostudy AT lauravilardellscholten efficiencyofbiofilmremovalbycombinationofwaterjetandcoldplasmaaninvitrostudy AT rabeaschluter efficiencyofbiofilmremovalbycombinationofwaterjetandcoldplasmaaninvitrostudy AT thomaskocher efficiencyofbiofilmremovalbycombinationofwaterjetandcoldplasmaaninvitrostudy |