Genome-Wide Expression Patterns of Rhoptry Kinases during the <i>Eimeria tenella</i> Life-Cycle
Kinome from apicomplexan parasites is composed of eukaryotic protein kinases and Apicomplexa specific kinases, such as rhoptry kinases (ROPK). <i>Ropk</i> is a gene family that is known to play important roles in host–pathogen interaction in <i>Toxoplasma gondii</i> but is st...
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MDPI AG
2021-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/8/1621 |
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| author | Adeline Ribeiro E Silva Alix Sausset Françoise I. Bussière Fabrice Laurent Sonia Lacroix-Lamandé Anne Silvestre |
| author_facet | Adeline Ribeiro E Silva Alix Sausset Françoise I. Bussière Fabrice Laurent Sonia Lacroix-Lamandé Anne Silvestre |
| author_sort | Adeline Ribeiro E Silva |
| collection | DOAJ |
| description | Kinome from apicomplexan parasites is composed of eukaryotic protein kinases and Apicomplexa specific kinases, such as rhoptry kinases (ROPK). <i>Ropk</i> is a gene family that is known to play important roles in host–pathogen interaction in <i>Toxoplasma gondii</i> but is still poorly described in <i>Eimeria tenella</i>, the parasite responsible for avian coccidiosis worldwide. In the <i>E. tenella</i> genome, 28 <i>ropk</i> genes are predicted and could be classified as active (<i>n</i> = 7), inactive (incomplete catalytic triad, <i>n</i> = 12), and non-canonical kinases (active kinase with a modified catalytic triad, <i>n</i> = 9). We characterized the <i>ropk</i> gene expression patterns by real-time quantitative RT-PCR, normalized by parasite housekeeping genes, during the <i>E. tenella</i> life-cycle. Analyzed stages were: non-sporulated oocysts, sporulated oocysts, extracellular and intracellular sporozoites, immature and mature schizonts I, first- and second-generation merozoites, and gametes. Transcription of all those predicted <i>ropk</i> was confirmed. The mean intensity of transcription was higher in extracellular stages and 7–9 <i>ropk</i> were specifically transcribed in merozoites in comparison with sporozoites. Transcriptional profiles of intracellular stages were closely related to each other, suggesting a probable common role of ROPKs in hijacking signaling pathways and immune responses in infected cells. These results provide a solid basis for future functional analysis of ROPK from <i>E. tenella</i>. |
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| language | English |
| last_indexed | 2024-03-10T08:35:16Z |
| publishDate | 2021-07-01 |
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| series | Microorganisms |
| spelling | doaj.art-84c9e6aeb0fa47d184121a9140d4893a2023-11-22T08:46:26ZengMDPI AGMicroorganisms2076-26072021-07-0198162110.3390/microorganisms9081621Genome-Wide Expression Patterns of Rhoptry Kinases during the <i>Eimeria tenella</i> Life-CycleAdeline Ribeiro E Silva0Alix Sausset1Françoise I. Bussière2Fabrice Laurent3Sonia Lacroix-Lamandé4Anne Silvestre5Institut National de Recherche pour L’agriculture, L’alimentation et L’environnement (INRAE), Université de Tours, ISP, 37380 Nouzilly, FranceInstitut National de Recherche pour L’agriculture, L’alimentation et L’environnement (INRAE), Université de Tours, ISP, 37380 Nouzilly, FranceInstitut National de Recherche pour L’agriculture, L’alimentation et L’environnement (INRAE), Université de Tours, ISP, 37380 Nouzilly, FranceInstitut National de Recherche pour L’agriculture, L’alimentation et L’environnement (INRAE), Université de Tours, ISP, 37380 Nouzilly, FranceInstitut National de Recherche pour L’agriculture, L’alimentation et L’environnement (INRAE), Université de Tours, ISP, 37380 Nouzilly, FranceInstitut National de Recherche pour L’agriculture, L’alimentation et L’environnement (INRAE), Université de Tours, ISP, 37380 Nouzilly, FranceKinome from apicomplexan parasites is composed of eukaryotic protein kinases and Apicomplexa specific kinases, such as rhoptry kinases (ROPK). <i>Ropk</i> is a gene family that is known to play important roles in host–pathogen interaction in <i>Toxoplasma gondii</i> but is still poorly described in <i>Eimeria tenella</i>, the parasite responsible for avian coccidiosis worldwide. In the <i>E. tenella</i> genome, 28 <i>ropk</i> genes are predicted and could be classified as active (<i>n</i> = 7), inactive (incomplete catalytic triad, <i>n</i> = 12), and non-canonical kinases (active kinase with a modified catalytic triad, <i>n</i> = 9). We characterized the <i>ropk</i> gene expression patterns by real-time quantitative RT-PCR, normalized by parasite housekeeping genes, during the <i>E. tenella</i> life-cycle. Analyzed stages were: non-sporulated oocysts, sporulated oocysts, extracellular and intracellular sporozoites, immature and mature schizonts I, first- and second-generation merozoites, and gametes. Transcription of all those predicted <i>ropk</i> was confirmed. The mean intensity of transcription was higher in extracellular stages and 7–9 <i>ropk</i> were specifically transcribed in merozoites in comparison with sporozoites. Transcriptional profiles of intracellular stages were closely related to each other, suggesting a probable common role of ROPKs in hijacking signaling pathways and immune responses in infected cells. These results provide a solid basis for future functional analysis of ROPK from <i>E. tenella</i>.https://www.mdpi.com/2076-2607/9/8/1621rhoptry kinasesgene expression<i>Eimeria tenella</i>life-cycle |
| spellingShingle | Adeline Ribeiro E Silva Alix Sausset Françoise I. Bussière Fabrice Laurent Sonia Lacroix-Lamandé Anne Silvestre Genome-Wide Expression Patterns of Rhoptry Kinases during the <i>Eimeria tenella</i> Life-Cycle Microorganisms rhoptry kinases gene expression <i>Eimeria tenella</i> life-cycle |
| title | Genome-Wide Expression Patterns of Rhoptry Kinases during the <i>Eimeria tenella</i> Life-Cycle |
| title_full | Genome-Wide Expression Patterns of Rhoptry Kinases during the <i>Eimeria tenella</i> Life-Cycle |
| title_fullStr | Genome-Wide Expression Patterns of Rhoptry Kinases during the <i>Eimeria tenella</i> Life-Cycle |
| title_full_unstemmed | Genome-Wide Expression Patterns of Rhoptry Kinases during the <i>Eimeria tenella</i> Life-Cycle |
| title_short | Genome-Wide Expression Patterns of Rhoptry Kinases during the <i>Eimeria tenella</i> Life-Cycle |
| title_sort | genome wide expression patterns of rhoptry kinases during the i eimeria tenella i life cycle |
| topic | rhoptry kinases gene expression <i>Eimeria tenella</i> life-cycle |
| url | https://www.mdpi.com/2076-2607/9/8/1621 |
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