SMC1A facilitates gastric cancer cell proliferation, migration, and invasion via promoting SNAIL activated EMT
Abstract Background Structural maintenance of chromosomes protein 1 A (SMC1A) is a crucial subunit of the cohesion protein complex and plays a vital role in cell cycle regulation, genomic stability maintenance, chromosome dynamics. Recent studies demonstrated that SMC1A participates in tumorigenesis...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | BMC Gastroenterology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12876-023-02850-z |
| _version_ | 1797559341048397824 |
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| author | Yaling Liu Xianrui Fang Qianqian Wang Da Xiao Ting Zhou Kuo Kang Zhenyu Peng Feng Ren Jingyu Zhou |
| author_facet | Yaling Liu Xianrui Fang Qianqian Wang Da Xiao Ting Zhou Kuo Kang Zhenyu Peng Feng Ren Jingyu Zhou |
| author_sort | Yaling Liu |
| collection | DOAJ |
| description | Abstract Background Structural maintenance of chromosomes protein 1 A (SMC1A) is a crucial subunit of the cohesion protein complex and plays a vital role in cell cycle regulation, genomic stability maintenance, chromosome dynamics. Recent studies demonstrated that SMC1A participates in tumorigenesis. This reseach aims to explore the role and the underlying mechanisms of SMC1A in gastric cancer (GC). Materials and methods RT-qPCR and western blot were used to examine the expression levels of SMC1A in GC tissues and cell lines. The role of SMC1A on GC cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) were analyzed. Furthermore,the mechanism of SMC1A action was investigated. Results SMC1A was highly expressed in GC tissues and cell lines. The high expression of SMC1A indicated the poor overall survival of GC patients from Kaplan-Meier Plotter. Enhancing the expression of SMC1A in AGS cells remarkably promoted cell proliferation in vitro and in vivo, migration and invasion, Conversely, knockdown of SMC1A in HGC27 cells inhibited cell proliferation, migration and invasion. Moreover, it’s observed that SMC1A promoted EMT and malignant cell behaviors via regulating SNAIL. Conclusion Our study revealed that SMC1A promotes EMT process by upregulating SNAIL, which contributes to gastric cancer cell proliferation, migration and invasion. Therefore, targeting SMC1A may be a potential strategy to improve GC therapy. |
| first_indexed | 2024-03-10T17:43:55Z |
| format | Article |
| id | doaj.art-84ccd4c3ecb84abf8763adcc0a8495af |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-03-10T17:43:55Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-84ccd4c3ecb84abf8763adcc0a8495af2023-11-20T09:35:58ZengBMCBMC Gastroenterology1471-230X2023-08-0123111010.1186/s12876-023-02850-zSMC1A facilitates gastric cancer cell proliferation, migration, and invasion via promoting SNAIL activated EMTYaling Liu0Xianrui Fang1Qianqian Wang2Da Xiao3Ting Zhou4Kuo Kang5Zhenyu Peng6Feng Ren7Jingyu Zhou8Department of Geriatrics Surgery, The Second Xiangya Hospital, Central South UniversityDepartment of General Surgery, Yantai Qishan HospitalDepartment of Oncology, The Affiliated ZhuZhou Hospital of XiangYa Medical College, Central South UniversityDepartment of General Surgery, Shekou People’s HospitalDepartment of Geriatrics Surgery, The Second Xiangya Hospital, Central South UniversityDepartment of Geriatrics Surgery, The Second Xiangya Hospital, Central South UniversityDepartment of Geriatrics Surgery, The Second Xiangya Hospital, Central South UniversityDepartment of Geriatrics Surgery, The Second Xiangya Hospital, Central South UniversityDepartment of Geriatrics Surgery, The Second Xiangya Hospital, Central South UniversityAbstract Background Structural maintenance of chromosomes protein 1 A (SMC1A) is a crucial subunit of the cohesion protein complex and plays a vital role in cell cycle regulation, genomic stability maintenance, chromosome dynamics. Recent studies demonstrated that SMC1A participates in tumorigenesis. This reseach aims to explore the role and the underlying mechanisms of SMC1A in gastric cancer (GC). Materials and methods RT-qPCR and western blot were used to examine the expression levels of SMC1A in GC tissues and cell lines. The role of SMC1A on GC cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) were analyzed. Furthermore,the mechanism of SMC1A action was investigated. Results SMC1A was highly expressed in GC tissues and cell lines. The high expression of SMC1A indicated the poor overall survival of GC patients from Kaplan-Meier Plotter. Enhancing the expression of SMC1A in AGS cells remarkably promoted cell proliferation in vitro and in vivo, migration and invasion, Conversely, knockdown of SMC1A in HGC27 cells inhibited cell proliferation, migration and invasion. Moreover, it’s observed that SMC1A promoted EMT and malignant cell behaviors via regulating SNAIL. Conclusion Our study revealed that SMC1A promotes EMT process by upregulating SNAIL, which contributes to gastric cancer cell proliferation, migration and invasion. Therefore, targeting SMC1A may be a potential strategy to improve GC therapy.https://doi.org/10.1186/s12876-023-02850-zGastric cancerStructural maintenance of chromosomes protein 1ASNAILEpithelial-mesenchymal transition |
| spellingShingle | Yaling Liu Xianrui Fang Qianqian Wang Da Xiao Ting Zhou Kuo Kang Zhenyu Peng Feng Ren Jingyu Zhou SMC1A facilitates gastric cancer cell proliferation, migration, and invasion via promoting SNAIL activated EMT BMC Gastroenterology Gastric cancer Structural maintenance of chromosomes protein 1A SNAIL Epithelial-mesenchymal transition |
| title | SMC1A facilitates gastric cancer cell proliferation, migration, and invasion via promoting SNAIL activated EMT |
| title_full | SMC1A facilitates gastric cancer cell proliferation, migration, and invasion via promoting SNAIL activated EMT |
| title_fullStr | SMC1A facilitates gastric cancer cell proliferation, migration, and invasion via promoting SNAIL activated EMT |
| title_full_unstemmed | SMC1A facilitates gastric cancer cell proliferation, migration, and invasion via promoting SNAIL activated EMT |
| title_short | SMC1A facilitates gastric cancer cell proliferation, migration, and invasion via promoting SNAIL activated EMT |
| title_sort | smc1a facilitates gastric cancer cell proliferation migration and invasion via promoting snail activated emt |
| topic | Gastric cancer Structural maintenance of chromosomes protein 1A SNAIL Epithelial-mesenchymal transition |
| url | https://doi.org/10.1186/s12876-023-02850-z |
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