Melatonin Treatment Maintains the Quality of Fresh-Cut <i>Gastrodia elata</i> under Low-Temperature Conditions by Regulating Reactive Oxygen Species Metabolism and Phenylpropanoid Pathway
The application of melatonin (MT) has been shown to improve the quality during the storage of fruits and vegetables. The primary objective of this study is to investigate the effects of MT on the quality of fresh-cut <i>Gastrodia elata</i> during low-temperature (4 °C) storage. The resul...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/14284 |
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| author | Boyu Dong Fangfang Da Yulong Chen Xiaochun Ding |
| author_facet | Boyu Dong Fangfang Da Yulong Chen Xiaochun Ding |
| author_sort | Boyu Dong |
| collection | DOAJ |
| description | The application of melatonin (MT) has been shown to improve the quality during the storage of fruits and vegetables. The primary objective of this study is to investigate the effects of MT on the quality of fresh-cut <i>Gastrodia elata</i> during low-temperature (4 °C) storage. The results indicated that MT treatment not only suppressed the respiratory rate and malondialdehyde content but also slowed down the decline in total acidity and total soluble solids, effectively inhibiting microbial growth and enhancing the product safety of fresh-cut <i>G. elata</i>. The treatment with MT reduced the superoxide anions and hydrogen peroxide production, as well as inhibiting the activity and expression of peroxidase and polyphenol oxidase. Additionally, it led to increased activity and the expression of antioxidant-related enzymes, including superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase, while also resulting in elevated levels of ascorbic acid and glutathione. Furthermore, the treatment with MT induced an increase in the total phenolic and flavonoid content of fresh-cut <i>G. elata</i> and enhanced the activity and expression of key enzymes involved in the phenylpropanoid pathway (phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, 4-coumarate: CoA ligase). In summary, MT enhances the antioxidant capacity by activating both the ROS metabolism and phenylpropanoid pathway, thus maintaining the quality of fresh-cut <i>G. elata</i>. |
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| id | doaj.art-84ce68d628d54fb780ae4fa66ad9575a |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:39:16Z |
| publishDate | 2023-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-84ce68d628d54fb780ae4fa66ad9575a2023-11-19T11:10:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181428410.3390/ijms241814284Melatonin Treatment Maintains the Quality of Fresh-Cut <i>Gastrodia elata</i> under Low-Temperature Conditions by Regulating Reactive Oxygen Species Metabolism and Phenylpropanoid PathwayBoyu Dong0Fangfang Da1Yulong Chen2Xiaochun Ding3School of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, ChinaSchool of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, ChinaSchool of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, ChinaEngineering Research Center for Fruit Crops of Guizhou Province, Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Agriculture, Guizhou University, Guiyang 550025, ChinaThe application of melatonin (MT) has been shown to improve the quality during the storage of fruits and vegetables. The primary objective of this study is to investigate the effects of MT on the quality of fresh-cut <i>Gastrodia elata</i> during low-temperature (4 °C) storage. The results indicated that MT treatment not only suppressed the respiratory rate and malondialdehyde content but also slowed down the decline in total acidity and total soluble solids, effectively inhibiting microbial growth and enhancing the product safety of fresh-cut <i>G. elata</i>. The treatment with MT reduced the superoxide anions and hydrogen peroxide production, as well as inhibiting the activity and expression of peroxidase and polyphenol oxidase. Additionally, it led to increased activity and the expression of antioxidant-related enzymes, including superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase, while also resulting in elevated levels of ascorbic acid and glutathione. Furthermore, the treatment with MT induced an increase in the total phenolic and flavonoid content of fresh-cut <i>G. elata</i> and enhanced the activity and expression of key enzymes involved in the phenylpropanoid pathway (phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, 4-coumarate: CoA ligase). In summary, MT enhances the antioxidant capacity by activating both the ROS metabolism and phenylpropanoid pathway, thus maintaining the quality of fresh-cut <i>G. elata</i>.https://www.mdpi.com/1422-0067/24/18/14284fresh-cut <i>Gastrodia elata</i>melatoninROS metabolismphenylpropanoid pathway |
| spellingShingle | Boyu Dong Fangfang Da Yulong Chen Xiaochun Ding Melatonin Treatment Maintains the Quality of Fresh-Cut <i>Gastrodia elata</i> under Low-Temperature Conditions by Regulating Reactive Oxygen Species Metabolism and Phenylpropanoid Pathway International Journal of Molecular Sciences fresh-cut <i>Gastrodia elata</i> melatonin ROS metabolism phenylpropanoid pathway |
| title | Melatonin Treatment Maintains the Quality of Fresh-Cut <i>Gastrodia elata</i> under Low-Temperature Conditions by Regulating Reactive Oxygen Species Metabolism and Phenylpropanoid Pathway |
| title_full | Melatonin Treatment Maintains the Quality of Fresh-Cut <i>Gastrodia elata</i> under Low-Temperature Conditions by Regulating Reactive Oxygen Species Metabolism and Phenylpropanoid Pathway |
| title_fullStr | Melatonin Treatment Maintains the Quality of Fresh-Cut <i>Gastrodia elata</i> under Low-Temperature Conditions by Regulating Reactive Oxygen Species Metabolism and Phenylpropanoid Pathway |
| title_full_unstemmed | Melatonin Treatment Maintains the Quality of Fresh-Cut <i>Gastrodia elata</i> under Low-Temperature Conditions by Regulating Reactive Oxygen Species Metabolism and Phenylpropanoid Pathway |
| title_short | Melatonin Treatment Maintains the Quality of Fresh-Cut <i>Gastrodia elata</i> under Low-Temperature Conditions by Regulating Reactive Oxygen Species Metabolism and Phenylpropanoid Pathway |
| title_sort | melatonin treatment maintains the quality of fresh cut i gastrodia elata i under low temperature conditions by regulating reactive oxygen species metabolism and phenylpropanoid pathway |
| topic | fresh-cut <i>Gastrodia elata</i> melatonin ROS metabolism phenylpropanoid pathway |
| url | https://www.mdpi.com/1422-0067/24/18/14284 |
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