Evaluation of oxidative stress, 3-Nitrotyrosine, and HMGB-1 levels in patients with wet type Age-Related Macular Degeneration
Background: This study aims to compare serum HMGB-1, 3-nitrotyrosine (3-NT), TAS, TOS, and OSI levels in Wettype Age-Related Macular Degeneration (wAMD) patients and healthy controls to determine the correlation of these parameters with each other. Methods: Thirty patients with Wet-type Age-Related...
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| Format: | Article |
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Society of Medical Biochemists of Serbia, Belgrade
2022-01-01
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| Series: | Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2022/1452-82582203275R.pdf |
| _version_ | 1818514214919077888 |
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| author | Ramazan Zor Kürşad Sarı İsmail Yıldırım Biçer Gamze Güntürk İnayet Küçük Erkut Erşan Serpil Seydel Gönül Şeyda |
| author_facet | Ramazan Zor Kürşad Sarı İsmail Yıldırım Biçer Gamze Güntürk İnayet Küçük Erkut Erşan Serpil Seydel Gönül Şeyda |
| author_sort | Ramazan Zor Kürşad |
| collection | DOAJ |
| description | Background: This study aims to compare serum HMGB-1, 3-nitrotyrosine (3-NT), TAS, TOS, and OSI levels in Wettype Age-Related Macular Degeneration (wAMD) patients and healthy controls to determine the correlation of these parameters with each other. Methods: Thirty patients with Wet-type Age-Related Macular Degeneration (wAMD) and 27 healthy adults, as controls were enrolled in the study. We determined the TAS and TOS levels in serum samples of both groups using commercial kits on a microplate reader. Serum HMGB-1 and 3-NT levels were measured with the enzyme-linked immunosorbent assay method. Results: HMGB-1 levels were significantly higher in the patient group (137.51 pg/mL, p=0.001), while there was no difference between the two groups in serum 3-NT levels (p = 0.428). A statistically significant difference found in the levels of TOS and OSI (p = 0.001 and p = 0.045, respectively) between the patients and controls, however, no significant difference was observed between the groups in terms of TAS levels (p = 0.228). Conclusions: Oxidative stress and HMGB-1 levels were increased in wAMD patients and enhanced oxidative stress may be associated with increased tissue necrosis and inflammation. Thus administration of antioxidant treatment in addition to routine therapy should be considered in wAMD. |
| first_indexed | 2024-12-11T00:12:43Z |
| format | Article |
| id | doaj.art-84d2c72ff00f46d58da793581a2d42e5 |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-12-11T00:12:43Z |
| publishDate | 2022-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-84d2c72ff00f46d58da793581a2d42e52022-12-22T01:28:04ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662022-01-0141327528110.5937/jomb0-321891452-82582203275REvaluation of oxidative stress, 3-Nitrotyrosine, and HMGB-1 levels in patients with wet type Age-Related Macular DegenerationRamazan Zor Kürşad0Sarı İsmail1Yıldırım Biçer Gamze2Güntürk İnayet3Küçük Erkut4Erşan Serpil5Seydel Gönül Şeyda6Niğde Ömer Halisdemir University School of Medicine Department of Ophthalmology, Bor Yolu, Niğde, TurkeyNiğde Ömer Halisdemir University School of Medicine Department of Biochemistry, Bor Yolu, Niğde, TurkeyNiğde Ömer Halisdemir University School of Medicine Department of Ophthalmology, Bor Yolu, Niğde, TurkeyNiğde Ömer Halisdemir University, Healthcare Services, Zübeyde Hanım Health Services Vocational High School, Bor Yolu, Niğde, TurkeyNiğde Ömer Halisdemir University School of Medicine Department of Ophthalmology, Bor Yolu, Niğde, TurkeyNiğde Ömer Halisdemir University School of Medicine Department of Biochemistry, Bor Yolu, Niğde, TurkeyNiğde Ömer Halisdemir University School of Medicine Department of Medical Biochemistry, Bor Yolu, Niğde, TurkeyBackground: This study aims to compare serum HMGB-1, 3-nitrotyrosine (3-NT), TAS, TOS, and OSI levels in Wettype Age-Related Macular Degeneration (wAMD) patients and healthy controls to determine the correlation of these parameters with each other. Methods: Thirty patients with Wet-type Age-Related Macular Degeneration (wAMD) and 27 healthy adults, as controls were enrolled in the study. We determined the TAS and TOS levels in serum samples of both groups using commercial kits on a microplate reader. Serum HMGB-1 and 3-NT levels were measured with the enzyme-linked immunosorbent assay method. Results: HMGB-1 levels were significantly higher in the patient group (137.51 pg/mL, p=0.001), while there was no difference between the two groups in serum 3-NT levels (p = 0.428). A statistically significant difference found in the levels of TOS and OSI (p = 0.001 and p = 0.045, respectively) between the patients and controls, however, no significant difference was observed between the groups in terms of TAS levels (p = 0.228). Conclusions: Oxidative stress and HMGB-1 levels were increased in wAMD patients and enhanced oxidative stress may be associated with increased tissue necrosis and inflammation. Thus administration of antioxidant treatment in addition to routine therapy should be considered in wAMD.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2022/1452-82582203275R.pdfhmgb-13-nitrotyrosinetastososiwet type amd |
| spellingShingle | Ramazan Zor Kürşad Sarı İsmail Yıldırım Biçer Gamze Güntürk İnayet Küçük Erkut Erşan Serpil Seydel Gönül Şeyda Evaluation of oxidative stress, 3-Nitrotyrosine, and HMGB-1 levels in patients with wet type Age-Related Macular Degeneration Journal of Medical Biochemistry hmgb-1 3-nitrotyrosine tas tos osi wet type amd |
| title | Evaluation of oxidative stress, 3-Nitrotyrosine, and HMGB-1 levels in patients with wet type Age-Related Macular Degeneration |
| title_full | Evaluation of oxidative stress, 3-Nitrotyrosine, and HMGB-1 levels in patients with wet type Age-Related Macular Degeneration |
| title_fullStr | Evaluation of oxidative stress, 3-Nitrotyrosine, and HMGB-1 levels in patients with wet type Age-Related Macular Degeneration |
| title_full_unstemmed | Evaluation of oxidative stress, 3-Nitrotyrosine, and HMGB-1 levels in patients with wet type Age-Related Macular Degeneration |
| title_short | Evaluation of oxidative stress, 3-Nitrotyrosine, and HMGB-1 levels in patients with wet type Age-Related Macular Degeneration |
| title_sort | evaluation of oxidative stress 3 nitrotyrosine and hmgb 1 levels in patients with wet type age related macular degeneration |
| topic | hmgb-1 3-nitrotyrosine tas tos osi wet type amd |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2022/1452-82582203275R.pdf |
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