In Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media
A very high rate of multiple shoots was obtained from nodal explants of Photinia x fraseri on MS medium supplemented with a various concentration of Kin alone (1, 2, and 3) mgl-1alone and combination of Kin at (1, 2, and 3) mgl-1 with IBA or IAA at (0.2 and 0.4) mgl-1. The results show that the numb...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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University of Zakho
2014-12-01
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| Series: | Science Journal of University of Zakho |
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| Online Access: | https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/238 |
| _version_ | 1811314617297666048 |
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| author | Layla Sh. Mohammed AL-Mizory Ameena M. Hassan |
| author_facet | Layla Sh. Mohammed AL-Mizory Ameena M. Hassan |
| author_sort | Layla Sh. Mohammed AL-Mizory |
| collection | DOAJ |
| description | A very high rate of multiple shoots was obtained from nodal explants of Photinia x fraseri on MS medium supplemented with a various concentration of Kin alone (1, 2, and 3) mgl-1alone and combination of Kin at (1, 2, and 3) mgl-1 with IBA or IAA at (0.2 and 0.4) mgl-1. The results show that the number of shoots/ explant was significantly affected the levels of Kin alone and combination with IBA or IAA. The number of shoots (6.00 shoot/ explant) when Kin added to the medium at 3mgl-1 in which it was significantly different from other Kin concentrations. While the effect of different medium with the fixed and best concentration of Kin+ IBA and Kin+ IAA on the average shoot number and leaves number /explant of Photinia explant (node segment and shoot tips) The highest number of shoots (5.90) was recorded in node segment when cultured on WPM containing Kin+ IBA and it was increased significantly compared with all treatments from shoot tip. However, the maximum number of leaves (20.10) was recorded on WPM medium supplemented with Kin+ IBA and it was increased significantly compared with all treatments which containing different media plus Kin at 2mgl-1 + 0.4mgl-1 IBA and Kin at 1mgl-1+ 0.4mgl-1 IAA.Shootlel form the combination of from photinia cultured on ¼MS medium improved 0.6 mgl-1NAA concentration produced the highest number of roots/shootlel (6.5roots/shootlet) compared with all treatments contains ½MS medium added IAA, IBA and NAA on all concentration. While, theshootlets from photinia cultured on ¼MS medium and supplemented with 0.6mgl-1NAA formed an average (5.29 cm) compared to all treatments containing ½MS medium. Such plantlets were successfully transferred to soil after hardening with a high rate of survival. |
| first_indexed | 2024-04-13T11:15:41Z |
| format | Article |
| id | doaj.art-84d461c1e4b34bc2860ae752368222e4 |
| institution | Directory Open Access Journal |
| issn | 2663-628X 2663-6298 |
| language | English |
| last_indexed | 2024-04-13T11:15:41Z |
| publishDate | 2014-12-01 |
| publisher | University of Zakho |
| record_format | Article |
| series | Science Journal of University of Zakho |
| spelling | doaj.art-84d461c1e4b34bc2860ae752368222e42022-12-22T02:48:59ZengUniversity of ZakhoScience Journal of University of Zakho2663-628X2663-62982014-12-0122310322238In Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture MediaLayla Sh. Mohammed AL-Mizory0Ameena M. Hassan1Dohuk UniversityDuhok UniversityA very high rate of multiple shoots was obtained from nodal explants of Photinia x fraseri on MS medium supplemented with a various concentration of Kin alone (1, 2, and 3) mgl-1alone and combination of Kin at (1, 2, and 3) mgl-1 with IBA or IAA at (0.2 and 0.4) mgl-1. The results show that the number of shoots/ explant was significantly affected the levels of Kin alone and combination with IBA or IAA. The number of shoots (6.00 shoot/ explant) when Kin added to the medium at 3mgl-1 in which it was significantly different from other Kin concentrations. While the effect of different medium with the fixed and best concentration of Kin+ IBA and Kin+ IAA on the average shoot number and leaves number /explant of Photinia explant (node segment and shoot tips) The highest number of shoots (5.90) was recorded in node segment when cultured on WPM containing Kin+ IBA and it was increased significantly compared with all treatments from shoot tip. However, the maximum number of leaves (20.10) was recorded on WPM medium supplemented with Kin+ IBA and it was increased significantly compared with all treatments which containing different media plus Kin at 2mgl-1 + 0.4mgl-1 IBA and Kin at 1mgl-1+ 0.4mgl-1 IAA.Shootlel form the combination of from photinia cultured on ¼MS medium improved 0.6 mgl-1NAA concentration produced the highest number of roots/shootlel (6.5roots/shootlet) compared with all treatments contains ½MS medium added IAA, IBA and NAA on all concentration. While, theshootlets from photinia cultured on ¼MS medium and supplemented with 0.6mgl-1NAA formed an average (5.29 cm) compared to all treatments containing ½MS medium. Such plantlets were successfully transferred to soil after hardening with a high rate of survival.https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/238Photinia x fraseritissue culture mediaplant growth regulators |
| spellingShingle | Layla Sh. Mohammed AL-Mizory Ameena M. Hassan In Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media Science Journal of University of Zakho Photinia x fraseri tissue culture media plant growth regulators |
| title | In Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media |
| title_full | In Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media |
| title_fullStr | In Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media |
| title_full_unstemmed | In Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media |
| title_short | In Vitro Multiplication of Photinia (Photinia X Fraseri)Usingdifferent Culture Media |
| title_sort | in vitro multiplication of photinia photinia x fraseri usingdifferent culture media |
| topic | Photinia x fraseri tissue culture media plant growth regulators |
| url | https://sjuoz.uoz.edu.krd/index.php/sjuoz/article/view/238 |
| work_keys_str_mv | AT laylashmohammedalmizory invitromultiplicationofphotiniaphotiniaxfraseriusingdifferentculturemedia AT ameenamhassan invitromultiplicationofphotiniaphotiniaxfraseriusingdifferentculturemedia |