Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells
Alokita Karmakar2, Stacie M Bratton1, Enkeleda Dervishi2, Anindya Ghosh3, Meena Mahmood2, Yang Xu2, Lamya Mohammed Saeed2, Thikra Mustafa2, Dan Casciano2, Anna Radominska-Pandya1, Alexandru S Biris21Biochemistry Department, University of Arkansas for Medical Sciences; 2Nanotechnology Center, Applied...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2011-05-01
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| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/ethylenediamine-functionalized-single-walled-nanotube-f-swnt-assisted--a7486 |
| _version_ | 1818693708428607488 |
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| author | Karmakar A Bratton SM Dervishi E Ghosh A Mahmood M Xu Y Saeed L Mustafa T Casciano D Radominska-Pandya A Biris AS |
| author_facet | Karmakar A Bratton SM Dervishi E Ghosh A Mahmood M Xu Y Saeed L Mustafa T Casciano D Radominska-Pandya A Biris AS |
| author_sort | Karmakar A |
| collection | DOAJ |
| description | Alokita Karmakar2, Stacie M Bratton1, Enkeleda Dervishi2, Anindya Ghosh3, Meena Mahmood2, Yang Xu2, Lamya Mohammed Saeed2, Thikra Mustafa2, Dan Casciano2, Anna Radominska-Pandya1, Alexandru S Biris21Biochemistry Department, University of Arkansas for Medical Sciences; 2Nanotechnology Center, Applied Science Department; 3Department of Chemistry, University of Arkansas, Little Rock, AR, USAAbstract: A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 µg mL-1 and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials.Keywords: carbon nanotubes, gene delivery, cancer cells, p53 oncogene suppressor |
| first_indexed | 2024-12-17T13:17:59Z |
| format | Article |
| id | doaj.art-84d647854c8d47919687049201c96301 |
| institution | Directory Open Access Journal |
| issn | 1176-9114 1178-2013 |
| language | English |
| last_indexed | 2024-12-17T13:17:59Z |
| publishDate | 2011-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-84d647854c8d47919687049201c963012022-12-21T21:46:57ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132011-05-012011default10451055Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cellsKarmakar ABratton SMDervishi EGhosh AMahmood MXu YSaeed LMustafa TCasciano DRadominska-Pandya ABiris ASAlokita Karmakar2, Stacie M Bratton1, Enkeleda Dervishi2, Anindya Ghosh3, Meena Mahmood2, Yang Xu2, Lamya Mohammed Saeed2, Thikra Mustafa2, Dan Casciano2, Anna Radominska-Pandya1, Alexandru S Biris21Biochemistry Department, University of Arkansas for Medical Sciences; 2Nanotechnology Center, Applied Science Department; 3Department of Chemistry, University of Arkansas, Little Rock, AR, USAAbstract: A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 µg mL-1 and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials.Keywords: carbon nanotubes, gene delivery, cancer cells, p53 oncogene suppressorhttp://www.dovepress.com/ethylenediamine-functionalized-single-walled-nanotube-f-swnt-assisted--a7486 |
| spellingShingle | Karmakar A Bratton SM Dervishi E Ghosh A Mahmood M Xu Y Saeed L Mustafa T Casciano D Radominska-Pandya A Biris AS Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells International Journal of Nanomedicine |
| title | Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| title_full | Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| title_fullStr | Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| title_full_unstemmed | Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| title_short | Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| title_sort | ethylenediamine functionalized single walled nanotube f swnt assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer mcf 7 cells |
| url | http://www.dovepress.com/ethylenediamine-functionalized-single-walled-nanotube-f-swnt-assisted--a7486 |
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