Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups
A forensic validation study of the Early Access Huaxia™ Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations. The genotyping of DNA samples was consistent across a large range of template DNA concentrations, with complete profiles obtain...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press
2018-04-01
|
| Series: | Forensic Sciences Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20961790.2018.1428782 |
| _version_ | 1797714788247142400 |
|---|---|
| author | Xiang Sheng Yali Wang Jiashuo Zhang Liqin Chen Yuan Lin Zhenmin Zhao Chengtao Li Suhua Zhang |
| author_facet | Xiang Sheng Yali Wang Jiashuo Zhang Liqin Chen Yuan Lin Zhenmin Zhao Chengtao Li Suhua Zhang |
| author_sort | Xiang Sheng |
| collection | DOAJ |
| description | A forensic validation study of the Early Access Huaxia™ Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations. The genotyping of DNA samples was consistent across a large range of template DNA concentrations, with complete profiles obtained at 0.125 ng; however, no more than 2 mm × 1.2 mm punches of samples would be recommended for direct amplification. The size precision and accuracy test revealed the genotyping ability; while consistent results were obtained when comparing the kit with other commercially available systems. In addition, the whole PCR amplification can finish within approximately 45 min, making the system suitable for fast-detection. However, only partial profiles may be obtained with challenging samples, including DNA stored on Foam-Tipped Applicators (FTA) cards or some case samples. For the forensic application in ethnic groups, a total of 282 and 229 alleles were obtained in Han and Mongolia, respectively. Since the 23 short tandem repeats were independent from each other, the cumulative power of exclusion in duos was 0.999 999 157 188 and the cumulative power of exclusion in trios was 0.999 999 999 859 in the Han group while the cumulative power of exclusion in duos (CPEduo) was 0.999 998 848 26 and cumulative power of exclusion in trios (CPEtrio) was 0.999 999 999 79 in the Mongolia group. And good internal consistency was found between the two investigated groups and the Sichuan Han, Hui, Tibetan and Uygur according to available reference data. |
| first_indexed | 2024-03-12T07:56:57Z |
| format | Article |
| id | doaj.art-84d6d8fa882f4b78aaf63c6f6c733355 |
| institution | Directory Open Access Journal |
| issn | 2096-1790 2471-1411 |
| language | English |
| last_indexed | 2024-03-12T07:56:57Z |
| publishDate | 2018-04-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Forensic Sciences Research |
| spelling | doaj.art-84d6d8fa882f4b78aaf63c6f6c7333552023-09-02T20:07:37ZengOxford University PressForensic Sciences Research2096-17902471-14112018-04-013213814410.1080/20961790.2018.14287821428782Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groupsXiang Sheng0Yali Wang1Jiashuo Zhang2Liqin Chen3Yuan Lin4Zhenmin Zhao5Chengtao Li6Suhua Zhang7Academy of Forensic ScienceAcademy of Forensic ScienceAcademy of Forensic ScienceInner Mongolia Medical UniversityAcademy of Forensic ScienceAcademy of Forensic ScienceAcademy of Forensic ScienceAcademy of Forensic ScienceA forensic validation study of the Early Access Huaxia™ Platinum Polymerase Chain Reaction (PCR) kit was completed to document the performance capabilities and limitations. The genotyping of DNA samples was consistent across a large range of template DNA concentrations, with complete profiles obtained at 0.125 ng; however, no more than 2 mm × 1.2 mm punches of samples would be recommended for direct amplification. The size precision and accuracy test revealed the genotyping ability; while consistent results were obtained when comparing the kit with other commercially available systems. In addition, the whole PCR amplification can finish within approximately 45 min, making the system suitable for fast-detection. However, only partial profiles may be obtained with challenging samples, including DNA stored on Foam-Tipped Applicators (FTA) cards or some case samples. For the forensic application in ethnic groups, a total of 282 and 229 alleles were obtained in Han and Mongolia, respectively. Since the 23 short tandem repeats were independent from each other, the cumulative power of exclusion in duos was 0.999 999 157 188 and the cumulative power of exclusion in trios was 0.999 999 999 859 in the Han group while the cumulative power of exclusion in duos (CPEduo) was 0.999 998 848 26 and cumulative power of exclusion in trios (CPEtrio) was 0.999 999 999 79 in the Mongolia group. And good internal consistency was found between the two investigated groups and the Sichuan Han, Hui, Tibetan and Uygur according to available reference data.http://dx.doi.org/10.1080/20961790.2018.1428782Forensic geneticsshort tandem repeats (STRs)Early Access Huaxia™ Platinum PCR kit |
| spellingShingle | Xiang Sheng Yali Wang Jiashuo Zhang Liqin Chen Yuan Lin Zhenmin Zhao Chengtao Li Suhua Zhang Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups Forensic Sciences Research Forensic genetics short tandem repeats (STRs) Early Access Huaxia™ Platinum PCR kit |
| title | Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups |
| title_full | Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups |
| title_fullStr | Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups |
| title_full_unstemmed | Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups |
| title_short | Forensic investigation of 23 autosomal STRs and application in Han and Mongolia ethnic groups |
| title_sort | forensic investigation of 23 autosomal strs and application in han and mongolia ethnic groups |
| topic | Forensic genetics short tandem repeats (STRs) Early Access Huaxia™ Platinum PCR kit |
| url | http://dx.doi.org/10.1080/20961790.2018.1428782 |
| work_keys_str_mv | AT xiangsheng forensicinvestigationof23autosomalstrsandapplicationinhanandmongoliaethnicgroups AT yaliwang forensicinvestigationof23autosomalstrsandapplicationinhanandmongoliaethnicgroups AT jiashuozhang forensicinvestigationof23autosomalstrsandapplicationinhanandmongoliaethnicgroups AT liqinchen forensicinvestigationof23autosomalstrsandapplicationinhanandmongoliaethnicgroups AT yuanlin forensicinvestigationof23autosomalstrsandapplicationinhanandmongoliaethnicgroups AT zhenminzhao forensicinvestigationof23autosomalstrsandapplicationinhanandmongoliaethnicgroups AT chengtaoli forensicinvestigationof23autosomalstrsandapplicationinhanandmongoliaethnicgroups AT suhuazhang forensicinvestigationof23autosomalstrsandapplicationinhanandmongoliaethnicgroups |