Effects of Chronic Administration of Capsaicin on Biomarkers of Kidney Injury in Male Wistar Rats with Experimental Diabetes
Capsaicin is an agonist of the transient receptor potential vanilloid type 1 (TRPV1) channel, which has been related to the pathophysiology of kidney disease secondary to diabetes. This study aimed to evaluate the chronic effect of capsaicin administration on biomarkers of kidney injury in an experi...
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| Format: | Article |
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MDPI AG
2018-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/24/1/36 |
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| author | Mónica Ríos-Silva Rubén Santos-Álvarez Xóchitl Trujillo Rosa Yolitzy Cárdenas-María Marisa López-Zamudio Jaime Alberto Bricio-Barrios Caridad Leal Alfredo Saavedra-Molina Miguel Huerta-Trujillo Karina Espinoza-Mejía Miguel Huerta |
| author_facet | Mónica Ríos-Silva Rubén Santos-Álvarez Xóchitl Trujillo Rosa Yolitzy Cárdenas-María Marisa López-Zamudio Jaime Alberto Bricio-Barrios Caridad Leal Alfredo Saavedra-Molina Miguel Huerta-Trujillo Karina Espinoza-Mejía Miguel Huerta |
| author_sort | Mónica Ríos-Silva |
| collection | DOAJ |
| description | Capsaicin is an agonist of the transient receptor potential vanilloid type 1 (TRPV1) channel, which has been related to the pathophysiology of kidney disease secondary to diabetes. This study aimed to evaluate the chronic effect of capsaicin administration on biomarkers of kidney injury in an experimental rat model of diabetes. Male Wistar rats were assigned to four groups: (1) healthy controls without diabetes (CON), (2) healthy controls plus capsaicin at 1 mg/kg/day (CON + CAPS), (3) experimental diabetes without capsaicin (DM), and (4) experimental diabetes plus capsaicin at 1 mg/kg/day (DM + CAPS). For each group, 24-h urine samples were collected to determine diuresis, albumin, cystatin C, β2 microglobulin, epidermal growth factor (EGF), alpha (1)-acid glycoprotein, and neutrophil gelatinase-associated lipocalin (NAG-L). Blood samples were drawn to measure fasting glucose. After 8 weeks, the CON + CAPS and DM + CAPS groups showed increased diuresis compared to the CON and DM groups, but the difference was significant only in the DM + CAPS group. The two-way ANOVA only showed a statistically significant effect of CAPS on the urinary EGF levels, as well as a tendency to have a significant effect in the urinary NAG-L levels. The EGF levels decreased in both CAPS-treated groups, but the change was only significant in the CON + CAPS group vs. CON group; and the NAG-L levels were lower in both CAPS-treated groups. These results show that capsaicin had a diuretic effect in healthy and diabetic rats; additionally, it increased the urinary EGF levels and tended to decrease the urinary NAG-L levels. |
| first_indexed | 2024-12-13T12:08:15Z |
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| id | doaj.art-84d7d102fe234974b0aa9c914c1151dd |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T12:08:15Z |
| publishDate | 2018-12-01 |
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| spelling | doaj.art-84d7d102fe234974b0aa9c914c1151dd2022-12-21T23:46:54ZengMDPI AGMolecules1420-30492018-12-012413610.3390/molecules24010036molecules24010036Effects of Chronic Administration of Capsaicin on Biomarkers of Kidney Injury in Male Wistar Rats with Experimental DiabetesMónica Ríos-Silva0Rubén Santos-Álvarez1Xóchitl Trujillo2Rosa Yolitzy Cárdenas-María3Marisa López-Zamudio4Jaime Alberto Bricio-Barrios5Caridad Leal6Alfredo Saavedra-Molina7Miguel Huerta-Trujillo8Karina Espinoza-Mejía9Miguel Huerta10Universidad de Colima, Centro Universitario de Investigaciones Biomédicas, Av. 25 de Julio No. 965, Col. Villas San Sebastián, Colima 28045, Colima, MexicoUniversidad de Colima, Centro Universitario de Investigaciones Biomédicas, Av. 25 de Julio No. 965, Col. Villas San Sebastián, Colima 28045, Colima, MexicoUniversidad de Colima, Centro Universitario de Investigaciones Biomédicas, Av. 25 de Julio No. 965, Col. Villas San Sebastián, Colima 28045, Colima, MexicoUniversidad de Colima, Centro Universitario de Investigaciones Biomédicas, Av. 25 de Julio No. 965, Col. Villas San Sebastián, Colima 28045, Colima, MexicoUniversidad de Colima, Centro Universitario de Investigaciones Biomédicas, Av. 25 de Julio No. 965, Col. Villas San Sebastián, Colima 28045, Colima, MexicoUniversidad de Colima, Facultad de Medicina, Escuela de Nutrición, Av. Universidad No. 333, Col. Las Víboras, Colima 28040, Colima, MexicoInstituto Mexicano del Seguro Social, Centro de Investigaciones Biomédicas de Occidente, Sierra Mojada No. 800, Col. Independencia, Guadalajara 44340, Jalisco, MexicoUniversidad Michoacana de San Nicolás de Hidalgo, Instituto de Investigaciones Químico-Biológicas; Av. Francisco J Mujica S/N, Morelia 58020, Michoacán, MexicoUniversidad de Colima, Facultad de Medicina, Av. Universidad No. 333, Col. Las Víboras, Colima 28040, Colima, MexicoUniversidad de Colima, Centro Universitario de Investigaciones Biomédicas, Av. 25 de Julio No. 965, Col. Villas San Sebastián, Colima 28045, Colima, MexicoUniversidad de Colima, Centro Universitario de Investigaciones Biomédicas, Av. 25 de Julio No. 965, Col. Villas San Sebastián, Colima 28045, Colima, MexicoCapsaicin is an agonist of the transient receptor potential vanilloid type 1 (TRPV1) channel, which has been related to the pathophysiology of kidney disease secondary to diabetes. This study aimed to evaluate the chronic effect of capsaicin administration on biomarkers of kidney injury in an experimental rat model of diabetes. Male Wistar rats were assigned to four groups: (1) healthy controls without diabetes (CON), (2) healthy controls plus capsaicin at 1 mg/kg/day (CON + CAPS), (3) experimental diabetes without capsaicin (DM), and (4) experimental diabetes plus capsaicin at 1 mg/kg/day (DM + CAPS). For each group, 24-h urine samples were collected to determine diuresis, albumin, cystatin C, β2 microglobulin, epidermal growth factor (EGF), alpha (1)-acid glycoprotein, and neutrophil gelatinase-associated lipocalin (NAG-L). Blood samples were drawn to measure fasting glucose. After 8 weeks, the CON + CAPS and DM + CAPS groups showed increased diuresis compared to the CON and DM groups, but the difference was significant only in the DM + CAPS group. The two-way ANOVA only showed a statistically significant effect of CAPS on the urinary EGF levels, as well as a tendency to have a significant effect in the urinary NAG-L levels. The EGF levels decreased in both CAPS-treated groups, but the change was only significant in the CON + CAPS group vs. CON group; and the NAG-L levels were lower in both CAPS-treated groups. These results show that capsaicin had a diuretic effect in healthy and diabetic rats; additionally, it increased the urinary EGF levels and tended to decrease the urinary NAG-L levels.http://www.mdpi.com/1420-3049/24/1/36TRPV1capsaicinrenal injurydiabetes mellitus |
| spellingShingle | Mónica Ríos-Silva Rubén Santos-Álvarez Xóchitl Trujillo Rosa Yolitzy Cárdenas-María Marisa López-Zamudio Jaime Alberto Bricio-Barrios Caridad Leal Alfredo Saavedra-Molina Miguel Huerta-Trujillo Karina Espinoza-Mejía Miguel Huerta Effects of Chronic Administration of Capsaicin on Biomarkers of Kidney Injury in Male Wistar Rats with Experimental Diabetes Molecules TRPV1 capsaicin renal injury diabetes mellitus |
| title | Effects of Chronic Administration of Capsaicin on Biomarkers of Kidney Injury in Male Wistar Rats with Experimental Diabetes |
| title_full | Effects of Chronic Administration of Capsaicin on Biomarkers of Kidney Injury in Male Wistar Rats with Experimental Diabetes |
| title_fullStr | Effects of Chronic Administration of Capsaicin on Biomarkers of Kidney Injury in Male Wistar Rats with Experimental Diabetes |
| title_full_unstemmed | Effects of Chronic Administration of Capsaicin on Biomarkers of Kidney Injury in Male Wistar Rats with Experimental Diabetes |
| title_short | Effects of Chronic Administration of Capsaicin on Biomarkers of Kidney Injury in Male Wistar Rats with Experimental Diabetes |
| title_sort | effects of chronic administration of capsaicin on biomarkers of kidney injury in male wistar rats with experimental diabetes |
| topic | TRPV1 capsaicin renal injury diabetes mellitus |
| url | http://www.mdpi.com/1420-3049/24/1/36 |
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