Effective Extraction of Limonene and Hibaene from Hinoki (<i>Chamaecyparis obtusa</i>) Using Ionic Liquid and Deep Eutectic Solvent
The essential oils of hinoki (<i>Chamaecyparis obtusa</i>) leaves have anti-bacterial, anti-fungal, and relaxation properties that are likely associated with the major components such as sabinene, α-terpinyl acetate, limonene, elemol, myrcene, and hibaene. The present study describes the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/14/4271 |
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| author | Rina Yasutomi Riki Anzawa Masamitsu Urakawa Toyonobu Usuki |
| author_facet | Rina Yasutomi Riki Anzawa Masamitsu Urakawa Toyonobu Usuki |
| author_sort | Rina Yasutomi |
| collection | DOAJ |
| description | The essential oils of hinoki (<i>Chamaecyparis obtusa</i>) leaves have anti-bacterial, anti-fungal, and relaxation properties that are likely associated with the major components such as sabinene, α-terpinyl acetate, limonene, elemol, myrcene, and hibaene. The present study describes the use of a cellulose-dissolving ionic liquid (IL) [C<sub>2</sub>mim][(MeO)(H)PO<sub>2</sub>] and low-toxicity solvents called betaine-based deep eutectic solvents (DESs) for the efficient extraction of hinoki essential oils. As a control method, organic solvent extraction was performed using either hexane, ethyl acetate (EtOAc), or acetone at 30 °C for 1 h. Both the experimental and control methods were conducted under the same conditions, which relied on partial dissolution of the leaves using the IL and DESs before partitioning the hinoki oils into the organic solvent for analysis. Quantitative analysis was performed using gas chromatography–mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode. The results indicated that extraction using the [C<sub>2</sub>mim][(MeO)(H)PO<sub>2</sub>]/acetone bilayer system improved the yields of limonene and hibaene, 1.5- and 1.9-fold, respectively, when compared with the control method. In addition, extraction using betaine/<span style="font-variant: small-caps;">l</span>-lactic acid (molar ratio 1:1) gave the greatest yields for both limonene and hibaene, 1.3-fold and 1.5-fold greater, respectively, than when using an organic solvent. These results demonstrate the effective extraction of essential oils from plant leaves under conditions milder than those needed for the conventional method. The less toxic and environmentally begin DESs for the extraction are also applicable to the food and cosmetic industries. |
| first_indexed | 2024-03-10T09:30:38Z |
| format | Article |
| id | doaj.art-84d8bdaabe894b3384e1aa37cb55df26 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T09:30:38Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-84d8bdaabe894b3384e1aa37cb55df262023-11-22T04:31:09ZengMDPI AGMolecules1420-30492021-07-012614427110.3390/molecules26144271Effective Extraction of Limonene and Hibaene from Hinoki (<i>Chamaecyparis obtusa</i>) Using Ionic Liquid and Deep Eutectic SolventRina Yasutomi0Riki Anzawa1Masamitsu Urakawa2Toyonobu Usuki3Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, JapanEbisu Kagaku Co. Ltd., 1-10-6 Kajicho, Chiyoda-ku, Tokyo 101-0044, JapanDepartment of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, JapanThe essential oils of hinoki (<i>Chamaecyparis obtusa</i>) leaves have anti-bacterial, anti-fungal, and relaxation properties that are likely associated with the major components such as sabinene, α-terpinyl acetate, limonene, elemol, myrcene, and hibaene. The present study describes the use of a cellulose-dissolving ionic liquid (IL) [C<sub>2</sub>mim][(MeO)(H)PO<sub>2</sub>] and low-toxicity solvents called betaine-based deep eutectic solvents (DESs) for the efficient extraction of hinoki essential oils. As a control method, organic solvent extraction was performed using either hexane, ethyl acetate (EtOAc), or acetone at 30 °C for 1 h. Both the experimental and control methods were conducted under the same conditions, which relied on partial dissolution of the leaves using the IL and DESs before partitioning the hinoki oils into the organic solvent for analysis. Quantitative analysis was performed using gas chromatography–mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode. The results indicated that extraction using the [C<sub>2</sub>mim][(MeO)(H)PO<sub>2</sub>]/acetone bilayer system improved the yields of limonene and hibaene, 1.5- and 1.9-fold, respectively, when compared with the control method. In addition, extraction using betaine/<span style="font-variant: small-caps;">l</span>-lactic acid (molar ratio 1:1) gave the greatest yields for both limonene and hibaene, 1.3-fold and 1.5-fold greater, respectively, than when using an organic solvent. These results demonstrate the effective extraction of essential oils from plant leaves under conditions milder than those needed for the conventional method. The less toxic and environmentally begin DESs for the extraction are also applicable to the food and cosmetic industries.https://www.mdpi.com/1420-3049/26/14/4271<i>Chamaecyparis obtusa</i>extractionhibaeneionic liquiddeep eutectic solvent |
| spellingShingle | Rina Yasutomi Riki Anzawa Masamitsu Urakawa Toyonobu Usuki Effective Extraction of Limonene and Hibaene from Hinoki (<i>Chamaecyparis obtusa</i>) Using Ionic Liquid and Deep Eutectic Solvent Molecules <i>Chamaecyparis obtusa</i> extraction hibaene ionic liquid deep eutectic solvent |
| title | Effective Extraction of Limonene and Hibaene from Hinoki (<i>Chamaecyparis obtusa</i>) Using Ionic Liquid and Deep Eutectic Solvent |
| title_full | Effective Extraction of Limonene and Hibaene from Hinoki (<i>Chamaecyparis obtusa</i>) Using Ionic Liquid and Deep Eutectic Solvent |
| title_fullStr | Effective Extraction of Limonene and Hibaene from Hinoki (<i>Chamaecyparis obtusa</i>) Using Ionic Liquid and Deep Eutectic Solvent |
| title_full_unstemmed | Effective Extraction of Limonene and Hibaene from Hinoki (<i>Chamaecyparis obtusa</i>) Using Ionic Liquid and Deep Eutectic Solvent |
| title_short | Effective Extraction of Limonene and Hibaene from Hinoki (<i>Chamaecyparis obtusa</i>) Using Ionic Liquid and Deep Eutectic Solvent |
| title_sort | effective extraction of limonene and hibaene from hinoki i chamaecyparis obtusa i using ionic liquid and deep eutectic solvent |
| topic | <i>Chamaecyparis obtusa</i> extraction hibaene ionic liquid deep eutectic solvent |
| url | https://www.mdpi.com/1420-3049/26/14/4271 |
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