Subcellular Distribution of Thyroid Hormone Receptor Beta in Ovarian Cancer
Background: Since the most well-known function of thyroid hormone receptors (TRs) relies on their ability to act as ligand-activated transcription factors, their subcellular localization has been recognized to be relevant for their biological meaning. The current study aimed to determine the prevale...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/5/2698 |
| _version_ | 1797474933729656832 |
|---|---|
| author | Sabine Heublein Udo Jeschke Cornelia Sattler Christina Kuhn Anna Hester Bastian Czogalla Fabian Trillsch Sven Mahner Doris Mayr Elisa Schmoeckel Nina Ditsch |
| author_facet | Sabine Heublein Udo Jeschke Cornelia Sattler Christina Kuhn Anna Hester Bastian Czogalla Fabian Trillsch Sven Mahner Doris Mayr Elisa Schmoeckel Nina Ditsch |
| author_sort | Sabine Heublein |
| collection | DOAJ |
| description | Background: Since the most well-known function of thyroid hormone receptors (TRs) relies on their ability to act as ligand-activated transcription factors, their subcellular localization has been recognized to be relevant for their biological meaning. The current study aimed to determine the prevalence and subcellular distribution of TR beta and TR beta-1 in ovarian cancer (OC). Methods: Tissue was collected from 153 patients that had undergone surgery due to OC at the Department of Obstetrics and Gynaecology of the Ludwig-Maximilians-University Munich. Immunohistochemistry detecting TR beta and TR beta-1 was performed. Staining signals were quantified and tested for association with clinico-pathological parameters including overall survival (OS). Results: The subcellular distribution of TR beta and TR beta-1 differed among histologic subtypes, grade and FIGO stage. TR beta positivity was strongly linked to shortened overall survival (<i>p</i> < 0.001). Strikingly, this shortened OS was mainly attributed to those cases showing complete (<i>p</i> = 0.005) or incomplete shift of TR beta to the cytoplasm (<i>p</i> < 0.001). Significance was lost in multivariate testing. Conclusions: Cytoplasmatic localization of TR beta was associated with reduced OS, at least in univariate analysis. Since TRs have long been supposed to mainly function via the regulation of gene transcription in the nucleus, cytoplasmatic shifting might be interpreted as a regulator of their activity. |
| first_indexed | 2024-03-09T20:37:03Z |
| format | Article |
| id | doaj.art-84dc7de1d0ca48c0b525fe231ce9bac8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:37:03Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-84dc7de1d0ca48c0b525fe231ce9bac82023-11-23T23:08:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01235269810.3390/ijms23052698Subcellular Distribution of Thyroid Hormone Receptor Beta in Ovarian CancerSabine Heublein0Udo Jeschke1Cornelia Sattler2Christina Kuhn3Anna Hester4Bastian Czogalla5Fabian Trillsch6Sven Mahner7Doris Mayr8Elisa Schmoeckel9Nina Ditsch10Department of Obstetrics and Gynecology, University of Heidelberg, 69120 Heidelberg, GermanyDepartment of Obstetrics and Gynecology, University Hospital LMU Munich, 81377 Munich, GermanyDepartment of Obstetrics and Gynecology, University of Heidelberg, 69120 Heidelberg, GermanyDepartment of Obstetrics and Gynecology, University Hospital LMU Munich, 81377 Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital LMU Munich, 81377 Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital LMU Munich, 81377 Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital LMU Munich, 81377 Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital LMU Munich, 81377 Munich, GermanyDepartment of Pathology, LMU Munich, 80337 Munich, GermanyDepartment of Pathology, LMU Munich, 80337 Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital LMU Munich, 81377 Munich, GermanyBackground: Since the most well-known function of thyroid hormone receptors (TRs) relies on their ability to act as ligand-activated transcription factors, their subcellular localization has been recognized to be relevant for their biological meaning. The current study aimed to determine the prevalence and subcellular distribution of TR beta and TR beta-1 in ovarian cancer (OC). Methods: Tissue was collected from 153 patients that had undergone surgery due to OC at the Department of Obstetrics and Gynaecology of the Ludwig-Maximilians-University Munich. Immunohistochemistry detecting TR beta and TR beta-1 was performed. Staining signals were quantified and tested for association with clinico-pathological parameters including overall survival (OS). Results: The subcellular distribution of TR beta and TR beta-1 differed among histologic subtypes, grade and FIGO stage. TR beta positivity was strongly linked to shortened overall survival (<i>p</i> < 0.001). Strikingly, this shortened OS was mainly attributed to those cases showing complete (<i>p</i> = 0.005) or incomplete shift of TR beta to the cytoplasm (<i>p</i> < 0.001). Significance was lost in multivariate testing. Conclusions: Cytoplasmatic localization of TR beta was associated with reduced OS, at least in univariate analysis. Since TRs have long been supposed to mainly function via the regulation of gene transcription in the nucleus, cytoplasmatic shifting might be interpreted as a regulator of their activity.https://www.mdpi.com/1422-0067/23/5/2698ovarian cancerthyroid hormone receptor betaprognosis |
| spellingShingle | Sabine Heublein Udo Jeschke Cornelia Sattler Christina Kuhn Anna Hester Bastian Czogalla Fabian Trillsch Sven Mahner Doris Mayr Elisa Schmoeckel Nina Ditsch Subcellular Distribution of Thyroid Hormone Receptor Beta in Ovarian Cancer International Journal of Molecular Sciences ovarian cancer thyroid hormone receptor beta prognosis |
| title | Subcellular Distribution of Thyroid Hormone Receptor Beta in Ovarian Cancer |
| title_full | Subcellular Distribution of Thyroid Hormone Receptor Beta in Ovarian Cancer |
| title_fullStr | Subcellular Distribution of Thyroid Hormone Receptor Beta in Ovarian Cancer |
| title_full_unstemmed | Subcellular Distribution of Thyroid Hormone Receptor Beta in Ovarian Cancer |
| title_short | Subcellular Distribution of Thyroid Hormone Receptor Beta in Ovarian Cancer |
| title_sort | subcellular distribution of thyroid hormone receptor beta in ovarian cancer |
| topic | ovarian cancer thyroid hormone receptor beta prognosis |
| url | https://www.mdpi.com/1422-0067/23/5/2698 |
| work_keys_str_mv | AT sabineheublein subcellulardistributionofthyroidhormonereceptorbetainovariancancer AT udojeschke subcellulardistributionofthyroidhormonereceptorbetainovariancancer AT corneliasattler subcellulardistributionofthyroidhormonereceptorbetainovariancancer AT christinakuhn subcellulardistributionofthyroidhormonereceptorbetainovariancancer AT annahester subcellulardistributionofthyroidhormonereceptorbetainovariancancer AT bastianczogalla subcellulardistributionofthyroidhormonereceptorbetainovariancancer AT fabiantrillsch subcellulardistributionofthyroidhormonereceptorbetainovariancancer AT svenmahner subcellulardistributionofthyroidhormonereceptorbetainovariancancer AT dorismayr subcellulardistributionofthyroidhormonereceptorbetainovariancancer AT elisaschmoeckel subcellulardistributionofthyroidhormonereceptorbetainovariancancer AT ninaditsch subcellulardistributionofthyroidhormonereceptorbetainovariancancer |