ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editing

ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editing

Abstract Background Identifying nuclease-induced double-stranded breaks in DNA on a genome-wide scale is critical for assessing the safety and efficacy of genome editing therapies. We previously demonstrated that after administering adeno-associated viral (AAV) vector-mediated genome-editing strateg...

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Bibliographic Details
Main Authors: Camilo Breton, Peter M. Clark, Lili Wang, Jenny A. Greig, James M. Wilson
Format: Article
Language:English
Published: BMC 2020-03-01
Series:BMC Genomics
Subjects:
Genome editing
Off-targets
Next-generation sequencing
In vivo
Editing
AAV integration
Online Access:http://link.springer.com/article/10.1186/s12864-020-6655-4

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