Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing
Abstract Blindness caused by corneal damage affects millions of people worldwide, and this number continues to rise. However, rapid epithelization and a stable epithelium process are the two biggest challenges for traditional corneal materials. These processes are related to corneal curvature, which...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-04-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202205878 |
| _version_ | 1827966899104776192 |
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| author | Yingni Xu Jia Liu Wenjing Song Qianchun Wang Xiaomin Sun Qi Zhao Yongrui Huang Haochen Li Yuehai Peng Jin Yuan Baohua Ji Li Ren |
| author_facet | Yingni Xu Jia Liu Wenjing Song Qianchun Wang Xiaomin Sun Qi Zhao Yongrui Huang Haochen Li Yuehai Peng Jin Yuan Baohua Ji Li Ren |
| author_sort | Yingni Xu |
| collection | DOAJ |
| description | Abstract Blindness caused by corneal damage affects millions of people worldwide, and this number continues to rise. However, rapid epithelization and a stable epithelium process are the two biggest challenges for traditional corneal materials. These processes are related to corneal curvature, which is an important factor in determination of the corneal healing process and epithelial behavior during corneal damage. In this study, smooth 3D‐printed convex corneal implants based on gelatin methacrylate and collagen are generated. As epithelium distribution and adhesion vary in different regions of the natural cornea, this work separates the surfaces into four regions and studies how cells sense topological cues on curvature. It is found that rabbit corneal epithelial cells (RCECs) seeded on steeper slope gradient surfaces on convex structures result in more aligned cell organization and tighter cell‐substrate adhesion, which can also be verified through finite element simulation and signaling pathway analysis. In vivo transplantation of convex implants result in a better fit with adjacent tissue and stronger cell adhesion than flat implants, thereby accelerating corneal epithelialization and promoting collagen fibers and neural regeneration within 180 days. Taken together, printed convex corneal implants that facilitate corneal regeneration may offer a translational strategy for the treatment of corneal damage. |
| first_indexed | 2024-04-09T17:56:55Z |
| format | Article |
| id | doaj.art-84dda761ba824cfc93721b92125bd2d2 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-09T17:56:55Z |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-84dda761ba824cfc93721b92125bd2d22023-04-14T19:54:19ZengWileyAdvanced Science2198-38442023-04-011011n/an/a10.1002/advs.202205878Biomimetic Convex Implant for Corneal Regeneration Through 3D PrintingYingni Xu0Jia Liu1Wenjing Song2Qianchun Wang3Xiaomin Sun4Qi Zhao5Yongrui Huang6Haochen Li7Yuehai Peng8Jin Yuan9Baohua Ji10Li Ren11School of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. ChinaSchool of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. ChinaSchool of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. ChinaWenzhou Institute University of Chinese Academy of Sciences Wenzhou 325001 P. R. ChinaSchool of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. ChinaSchool of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. ChinaSchool of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. ChinaSchool of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. ChinaNational Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. ChinaState Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat‐sen University Guangzhou 510623 P. R. ChinaInstitute of Biomechanics and Applications, Department of Engineering Mechanics Zhejiang University Hangzhou 310027 P. R. ChinaSchool of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and Reconstruction Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education Innovation Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 P. R. ChinaAbstract Blindness caused by corneal damage affects millions of people worldwide, and this number continues to rise. However, rapid epithelization and a stable epithelium process are the two biggest challenges for traditional corneal materials. These processes are related to corneal curvature, which is an important factor in determination of the corneal healing process and epithelial behavior during corneal damage. In this study, smooth 3D‐printed convex corneal implants based on gelatin methacrylate and collagen are generated. As epithelium distribution and adhesion vary in different regions of the natural cornea, this work separates the surfaces into four regions and studies how cells sense topological cues on curvature. It is found that rabbit corneal epithelial cells (RCECs) seeded on steeper slope gradient surfaces on convex structures result in more aligned cell organization and tighter cell‐substrate adhesion, which can also be verified through finite element simulation and signaling pathway analysis. In vivo transplantation of convex implants result in a better fit with adjacent tissue and stronger cell adhesion than flat implants, thereby accelerating corneal epithelialization and promoting collagen fibers and neural regeneration within 180 days. Taken together, printed convex corneal implants that facilitate corneal regeneration may offer a translational strategy for the treatment of corneal damage.https://doi.org/10.1002/advs.2022058783D printingslope gradientcell adhesioncellular mechanosensingcorneal regeneration |
| spellingShingle | Yingni Xu Jia Liu Wenjing Song Qianchun Wang Xiaomin Sun Qi Zhao Yongrui Huang Haochen Li Yuehai Peng Jin Yuan Baohua Ji Li Ren Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing Advanced Science 3D printing slope gradient cell adhesion cellular mechanosensing corneal regeneration |
| title | Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing |
| title_full | Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing |
| title_fullStr | Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing |
| title_full_unstemmed | Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing |
| title_short | Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing |
| title_sort | biomimetic convex implant for corneal regeneration through 3d printing |
| topic | 3D printing slope gradient cell adhesion cellular mechanosensing corneal regeneration |
| url | https://doi.org/10.1002/advs.202205878 |
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