Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice

Abstract There is no simple method to measure glomerular filtration rate (GFR) in mice, which limits the use of mice in models of renal diseases. We aimed at simplifying the plasma clearance of iohexol in mice, using dried blood spot (DBS) sampling in order to reduce the amount of blood taken for an...

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Main Authors: Ana Elena Rodríguez-Rodríguez, Sergio Luis-Lima, Javier Donate-Correa, Laura Diaz-Martín, María Rosa Arnau, Alejandro Jiménez-Sosa, Flavio Gaspari, Alberto Ortiz, Esteban Porrini
Format: Article
Language:English
Published: Nature Portfolio 2021-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-83934-2
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author Ana Elena Rodríguez-Rodríguez
Sergio Luis-Lima
Javier Donate-Correa
Laura Diaz-Martín
María Rosa Arnau
Alejandro Jiménez-Sosa
Flavio Gaspari
Alberto Ortiz
Esteban Porrini
author_facet Ana Elena Rodríguez-Rodríguez
Sergio Luis-Lima
Javier Donate-Correa
Laura Diaz-Martín
María Rosa Arnau
Alejandro Jiménez-Sosa
Flavio Gaspari
Alberto Ortiz
Esteban Porrini
author_sort Ana Elena Rodríguez-Rodríguez
collection DOAJ
description Abstract There is no simple method to measure glomerular filtration rate (GFR) in mice, which limits the use of mice in models of renal diseases. We aimed at simplifying the plasma clearance of iohexol in mice, using dried blood spot (DBS) sampling in order to reduce the amount of blood taken for analysis. GFR was measured simultaneously by a reference method in total blood—as described before—and tested method using DBS in fifteen male and six female C57BL/6J mice. Total blood extraction was 50 μL for the reference methods and 25μL for the tested methods, distributed in 5 samples. The agreement of GFR values between both methods was analyzed with the concordance correlation coefficient (CCC), total deviation index (TDI) and coverage probability (CP). The agreement between both methods was excellent, showing a TDI = 8.1%, which indicates that 90% of the GFR values obtained with DBS showed an error ranging from − 8 to + 8% of the reference method; a CCC of 0.996 (CI: 0.992), reflecting high precision and accuracy and a CP of 94 (CI: 83), indicating that 6% of the GFR values obtained with DBS had an error greater than 10% of the method in blood. So, both methods are interchangeable. DBS represent a major simplification of GFR measurement in mice. Also, DBS improves animal welfare by reducing the total blood required and refining the procedure.
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spelling doaj.art-84dec4fe7eb64ad9b3eb968af3079f7c2022-12-21T19:31:08ZengNature PortfolioScientific Reports2045-23222021-02-011111710.1038/s41598-021-83934-2Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious miceAna Elena Rodríguez-Rodríguez0Sergio Luis-Lima1Javier Donate-Correa2Laura Diaz-Martín3María Rosa Arnau4Alejandro Jiménez-Sosa5Flavio Gaspari6Alberto Ortiz7Esteban Porrini8Research Unit, Hospital Universitario de CanariasDepartment of Nephrology and Hypertension, IIS-Fundación Jimenez Díaz, UAMResearch Unit, Hospital Universitario de CanariasFundación Canaria Investigación Sanitaria de Canarias (FIISC)Servicio del Estabulario, Universidad de la LagunaFundación Canaria Investigación Sanitaria de Canarias (FIISC)Laboratorio de Función Renal, Instituto Tecnologías Biomédicas (ITB), Universidad de la LagunaDepartment of Nephrology and Hypertension, IIS-Fundación Jimenez Díaz, UAMNephrology Department, Unidad de Ensayos Clinicos-UCICEC. Hospital Universitario de CanariasAbstract There is no simple method to measure glomerular filtration rate (GFR) in mice, which limits the use of mice in models of renal diseases. We aimed at simplifying the plasma clearance of iohexol in mice, using dried blood spot (DBS) sampling in order to reduce the amount of blood taken for analysis. GFR was measured simultaneously by a reference method in total blood—as described before—and tested method using DBS in fifteen male and six female C57BL/6J mice. Total blood extraction was 50 μL for the reference methods and 25μL for the tested methods, distributed in 5 samples. The agreement of GFR values between both methods was analyzed with the concordance correlation coefficient (CCC), total deviation index (TDI) and coverage probability (CP). The agreement between both methods was excellent, showing a TDI = 8.1%, which indicates that 90% of the GFR values obtained with DBS showed an error ranging from − 8 to + 8% of the reference method; a CCC of 0.996 (CI: 0.992), reflecting high precision and accuracy and a CP of 94 (CI: 83), indicating that 6% of the GFR values obtained with DBS had an error greater than 10% of the method in blood. So, both methods are interchangeable. DBS represent a major simplification of GFR measurement in mice. Also, DBS improves animal welfare by reducing the total blood required and refining the procedure.https://doi.org/10.1038/s41598-021-83934-2
spellingShingle Ana Elena Rodríguez-Rodríguez
Sergio Luis-Lima
Javier Donate-Correa
Laura Diaz-Martín
María Rosa Arnau
Alejandro Jiménez-Sosa
Flavio Gaspari
Alberto Ortiz
Esteban Porrini
Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
Scientific Reports
title Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
title_full Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
title_fullStr Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
title_full_unstemmed Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
title_short Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
title_sort iohexol plasma clearance simplified by dried blood spot dbs sampling to measure renal function in conscious mice
url https://doi.org/10.1038/s41598-021-83934-2
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