Comparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE×WE) Japanese Quail Embryos
Sex reversal effects of 17 beta-estradiol (E2), diethylstilbestrol (DES) and ethynylestradiol (EE2) on male gonads in F1 (AWE×WE) Japanese quail (Corturnix japonica) embryos were comparatively evaluated in a newly developed in vivo screening model called as the sex reversal test. Male and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2005-05-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/42/2/42_2_119/_pdf/-char/en |
| _version_ | 1797816948322467840 |
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| author | Kazumoto Shibuya Makoto Mizutani Kazuo Sato Masafumi Itabashi Tetsuo Nunoya |
| author_facet | Kazumoto Shibuya Makoto Mizutani Kazuo Sato Masafumi Itabashi Tetsuo Nunoya |
| author_sort | Kazumoto Shibuya |
| collection | DOAJ |
| description | Sex reversal effects of 17 beta-estradiol (E2), diethylstilbestrol (DES) and ethynylestradiol (EE2) on male gonads in F1 (AWE×WE) Japanese quail (Corturnix japonica) embryos were comparatively evaluated in a newly developed in vivo screening model called as the sex reversal test. Male and female offspring of F1 (AWE×WE) Japanese quail exhibit exactly wild and albino plumage colors, respectively, ruled by a criss-cross inheritance. The natural and synthetic estrogens were injected into egg white just before the incubation. At 16 days of incubation, embryos were subjected by a complete necropsy and their gonads were grossly observed and examined histopathologically and morphometrically. Grossly, genetic sex confirmed by plumage colors coincided completely with external sex phenotype of the gonads in all embryos of the control group and E2 and DES-treated groups. However, several male embryos with wild plumage in the EE2 2000ng group possessed an ovary-like gonad in the left side and a vestigial right gonad. Histopathologically, E2, DES and EE2 exposures induced a dose-dependent sex reversal effect, i.e. ovotestis development, in the left testis. The left testes showing an ovary-like morphology in the EE2 2000ng group consisted of the most of area replaced with ovarian tissue and the small area of remaining testicular cords. The incidence and morphometric analysis of the ovotestis revealed that the order of potency of sex reversal effect in Japanese quail embryos was EE2>DES>E2. E2, DES and EE2 exposures induced no noticeable changes in the ovaries of any embryos. The present study suggests that the sex reversal test using F1 (AWE×WE) Japanese quail embryo is possible to evaluate feminization effects of endocrine disrupting chemicals with estrogenic activities in avian male embryos. |
| first_indexed | 2024-03-13T08:45:13Z |
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| id | doaj.art-84e610bc14c040829bb556b6d6417fcd |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:45:13Z |
| publishDate | 2005-05-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-84e610bc14c040829bb556b6d6417fcd2023-05-30T06:13:42ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862005-05-0142211912910.2141/jpsa.42.119jpsaComparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE×WE) Japanese Quail EmbryosKazumoto Shibuya0Makoto Mizutani1Kazuo Sato2Masafumi Itabashi3Tetsuo Nunoya4Nippon Institute for Biological ScienceNippon Institute for Biological ScienceNippon Institute for Biological ScienceNippon Institute for Biological ScienceNippon Institute for Biological ScienceSex reversal effects of 17 beta-estradiol (E2), diethylstilbestrol (DES) and ethynylestradiol (EE2) on male gonads in F1 (AWE×WE) Japanese quail (Corturnix japonica) embryos were comparatively evaluated in a newly developed in vivo screening model called as the sex reversal test. Male and female offspring of F1 (AWE×WE) Japanese quail exhibit exactly wild and albino plumage colors, respectively, ruled by a criss-cross inheritance. The natural and synthetic estrogens were injected into egg white just before the incubation. At 16 days of incubation, embryos were subjected by a complete necropsy and their gonads were grossly observed and examined histopathologically and morphometrically. Grossly, genetic sex confirmed by plumage colors coincided completely with external sex phenotype of the gonads in all embryos of the control group and E2 and DES-treated groups. However, several male embryos with wild plumage in the EE2 2000ng group possessed an ovary-like gonad in the left side and a vestigial right gonad. Histopathologically, E2, DES and EE2 exposures induced a dose-dependent sex reversal effect, i.e. ovotestis development, in the left testis. The left testes showing an ovary-like morphology in the EE2 2000ng group consisted of the most of area replaced with ovarian tissue and the small area of remaining testicular cords. The incidence and morphometric analysis of the ovotestis revealed that the order of potency of sex reversal effect in Japanese quail embryos was EE2>DES>E2. E2, DES and EE2 exposures induced no noticeable changes in the ovaries of any embryos. The present study suggests that the sex reversal test using F1 (AWE×WE) Japanese quail embryo is possible to evaluate feminization effects of endocrine disrupting chemicals with estrogenic activities in avian male embryos.https://www.jstage.jst.go.jp/article/jpsa/42/2/42_2_119/_pdf/-char/enendocrine disrupting effectsestrogensjapanese quail embryoovotestisscreening model |
| spellingShingle | Kazumoto Shibuya Makoto Mizutani Kazuo Sato Masafumi Itabashi Tetsuo Nunoya Comparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE×WE) Japanese Quail Embryos The Journal of Poultry Science endocrine disrupting effects estrogens japanese quail embryo ovotestis screening model |
| title | Comparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE×WE) Japanese Quail Embryos |
| title_full | Comparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE×WE) Japanese Quail Embryos |
| title_fullStr | Comparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE×WE) Japanese Quail Embryos |
| title_full_unstemmed | Comparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE×WE) Japanese Quail Embryos |
| title_short | Comparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE×WE) Japanese Quail Embryos |
| title_sort | comparative evaluation of sex reversal effects of natural and synthetic estrogens in sex reversal test using f1 awe times we japanese quail embryos |
| topic | endocrine disrupting effects estrogens japanese quail embryo ovotestis screening model |
| url | https://www.jstage.jst.go.jp/article/jpsa/42/2/42_2_119/_pdf/-char/en |
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