Characterization of the gsdf gene in channel catfish (Ictalurus punctatus): molecular cloning, tissue distribution, and expression patterns in the gonad after treatment with 17β-estradiol
The gonadal soma-derived factor (gsdf) gene was previously identified in a variety of fish and was found to be closely related to sexual differentiation and gonad development. In this study, gsdf was identified in channel catfish (Ictalurus punctatus) using a series of bioinformatics methods. Additi...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423002302 |
| _version_ | 1797752985732775936 |
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| author | Siqi Xu Shiyong Zhang Wenping Zhang Hongyan Liu Ju Liu Minghua Wang Liqiang Zhong Xiaohui Chen |
| author_facet | Siqi Xu Shiyong Zhang Wenping Zhang Hongyan Liu Ju Liu Minghua Wang Liqiang Zhong Xiaohui Chen |
| author_sort | Siqi Xu |
| collection | DOAJ |
| description | The gonadal soma-derived factor (gsdf) gene was previously identified in a variety of fish and was found to be closely related to sexual differentiation and gonad development. In this study, gsdf was identified in channel catfish (Ictalurus punctatus) using a series of bioinformatics methods. Additionally, artificial compound feed containing 17β-estradiol (60 μg/g) was used to induce the sexual reversal of male catfish, and then gsdf expression was compared among XX females, XY females, and XY males at the age of 90 and 540 days after hatching. Synteny analysis revealed that gsdf genes were relatively conserved in bony fish. Tissue distribution analysis showed that this gene was highly expressed mainly in the testis (P < 0.01). After 17β-estradiol treatment, gsdf expression was inhibited in XY female channel catfish. Its expression differed significantly between XY females and XY males (P < 0.01) but not between XY and XX females. In situ hybridization showed that gsdf mRNA was expressed in Sertoli cells of the testis but not in the ovary of normal female channel catfish, and only a small amount was detected in the ovary of XY female channel catfish. Results of this study provide a foundation for further analysis of the molecular mechanism by which gsdf affects sex differentiation and maintenance in channel catfish. |
| first_indexed | 2024-03-12T17:11:22Z |
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| id | doaj.art-84ef56e4f8364f78b1aa0eda75060119 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-12T17:11:22Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj.art-84ef56e4f8364f78b1aa0eda750601192023-08-06T04:37:45ZengElsevierAquaculture Reports2352-51342023-08-0131101691Characterization of the gsdf gene in channel catfish (Ictalurus punctatus): molecular cloning, tissue distribution, and expression patterns in the gonad after treatment with 17β-estradiolSiqi Xu0Shiyong Zhang1Wenping Zhang2Hongyan Liu3Ju Liu4Minghua Wang5Liqiang Zhong6Xiaohui Chen7National Genetic Breeding Center of Channel Catfish, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, ChinaNational Genetic Breeding Center of Channel Catfish, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, China; College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang, China; Corresponding authors at: National Genetic Breeding Center of Channel Catfish, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, China.National Genetic Breeding Center of Channel Catfish, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, China; College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang, ChinaNational Genetic Breeding Center of Channel Catfish, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, ChinaNational Genetic Breeding Center of Channel Catfish, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, ChinaNational Genetic Breeding Center of Channel Catfish, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, ChinaNational Genetic Breeding Center of Channel Catfish, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, ChinaNational Genetic Breeding Center of Channel Catfish, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, China; College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang, China; Corresponding authors at: National Genetic Breeding Center of Channel Catfish, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, China.The gonadal soma-derived factor (gsdf) gene was previously identified in a variety of fish and was found to be closely related to sexual differentiation and gonad development. In this study, gsdf was identified in channel catfish (Ictalurus punctatus) using a series of bioinformatics methods. Additionally, artificial compound feed containing 17β-estradiol (60 μg/g) was used to induce the sexual reversal of male catfish, and then gsdf expression was compared among XX females, XY females, and XY males at the age of 90 and 540 days after hatching. Synteny analysis revealed that gsdf genes were relatively conserved in bony fish. Tissue distribution analysis showed that this gene was highly expressed mainly in the testis (P < 0.01). After 17β-estradiol treatment, gsdf expression was inhibited in XY female channel catfish. Its expression differed significantly between XY females and XY males (P < 0.01) but not between XY and XX females. In situ hybridization showed that gsdf mRNA was expressed in Sertoli cells of the testis but not in the ovary of normal female channel catfish, and only a small amount was detected in the ovary of XY female channel catfish. Results of this study provide a foundation for further analysis of the molecular mechanism by which gsdf affects sex differentiation and maintenance in channel catfish.http://www.sciencedirect.com/science/article/pii/S2352513423002302gsdf17β-estradiolSex reversalChannel catfish |
| spellingShingle | Siqi Xu Shiyong Zhang Wenping Zhang Hongyan Liu Ju Liu Minghua Wang Liqiang Zhong Xiaohui Chen Characterization of the gsdf gene in channel catfish (Ictalurus punctatus): molecular cloning, tissue distribution, and expression patterns in the gonad after treatment with 17β-estradiol Aquaculture Reports gsdf 17β-estradiol Sex reversal Channel catfish |
| title | Characterization of the gsdf gene in channel catfish (Ictalurus punctatus): molecular cloning, tissue distribution, and expression patterns in the gonad after treatment with 17β-estradiol |
| title_full | Characterization of the gsdf gene in channel catfish (Ictalurus punctatus): molecular cloning, tissue distribution, and expression patterns in the gonad after treatment with 17β-estradiol |
| title_fullStr | Characterization of the gsdf gene in channel catfish (Ictalurus punctatus): molecular cloning, tissue distribution, and expression patterns in the gonad after treatment with 17β-estradiol |
| title_full_unstemmed | Characterization of the gsdf gene in channel catfish (Ictalurus punctatus): molecular cloning, tissue distribution, and expression patterns in the gonad after treatment with 17β-estradiol |
| title_short | Characterization of the gsdf gene in channel catfish (Ictalurus punctatus): molecular cloning, tissue distribution, and expression patterns in the gonad after treatment with 17β-estradiol |
| title_sort | characterization of the gsdf gene in channel catfish ictalurus punctatus molecular cloning tissue distribution and expression patterns in the gonad after treatment with 17β estradiol |
| topic | gsdf 17β-estradiol Sex reversal Channel catfish |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423002302 |
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