Novel Microsatellite Loci, Cross-Species Validation of Multiplex Assays, and By-Catch Mitochondrial Genomes on <i>Ochthebius</i> Beetles from Supratidal Rockpools
Here we focus on designing, for the first time, microsatellite markers for evolutionary and ecological research on aquatic beetles from the genus <i>Ochthebius</i> (Coleoptera, Hydraenidae). Some of these non-model species, with high cryptic diversity, exclusively inhabit supratidal rock...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/11/881 |
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| author | Antonio José García-Meseguer Adrián Villastrigo Juana María Mirón-Gatón Andrés Millán Josefa Velasco Irene Muñoz |
| author_facet | Antonio José García-Meseguer Adrián Villastrigo Juana María Mirón-Gatón Andrés Millán Josefa Velasco Irene Muñoz |
| author_sort | Antonio José García-Meseguer |
| collection | DOAJ |
| description | Here we focus on designing, for the first time, microsatellite markers for evolutionary and ecological research on aquatic beetles from the genus <i>Ochthebius</i> (Coleoptera, Hydraenidae). Some of these non-model species, with high cryptic diversity, exclusively inhabit supratidal rockpools, extreme and highly dynamic habitats with important anthropogenic threats. We analysed 15 individuals of four species (<i>O. lejolisii</i>, <i>O. subinteger</i>, <i>O. celatus</i>, and <i>O. quadricollis</i>) across 10 localities from the Mediterranean coasts of Spain and Malta. Using next-generation sequencing technology, two libraries were constructed to interpret the species of the two subgenera present consistently (<i>Ochthebius</i> s. str., <i>O. quadricollis</i>; and <i>Cobalius</i>, the rest of the species). Finally, 20 markers (10 for each subgenus) were obtained and successfully tested by cross-validation in the four species under study. As a by-catch, we could retrieve the complete mitochondrial genomes of <i>O. lejolisii</i>, <i>O. quadricollis</i>, and <i>O. subinteger</i>. Interestingly, the mitochondrial genome of <i>O. quadricollis</i> exhibited high genetic variability compared to already published data. The novel SSR panels and mitochondrial genomes for <i>Ochthebius</i> will be valuable in future research on species identification, diversity, genetic structure, and population connectivity in highly dynamic and threatened habitats such as supratidal coastal rockpools. |
| first_indexed | 2024-03-09T16:44:07Z |
| format | Article |
| id | doaj.art-84f23390074543bb9927b3a2da24238d |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T16:44:07Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-84f23390074543bb9927b3a2da24238d2023-11-24T14:48:42ZengMDPI AGInsects2075-44502023-11-01141188110.3390/insects14110881Novel Microsatellite Loci, Cross-Species Validation of Multiplex Assays, and By-Catch Mitochondrial Genomes on <i>Ochthebius</i> Beetles from Supratidal RockpoolsAntonio José García-Meseguer0Adrián Villastrigo1Juana María Mirón-Gatón2Andrés Millán3Josefa Velasco4Irene Muñoz5Ecology and Hydrology Department, University of Murcia, 30100 Murcia, SpainDivision of Entomology, SNSB-Zoologische Staatssammlung München, 81247 Munich, GermanyEcology and Hydrology Department, University of Murcia, 30100 Murcia, SpainEcology and Hydrology Department, University of Murcia, 30100 Murcia, SpainEcology and Hydrology Department, University of Murcia, 30100 Murcia, SpainDepartment of Biodiversity, Ecology and Evolution, Complutense University of Madrid, 28040 Madrid, SpainHere we focus on designing, for the first time, microsatellite markers for evolutionary and ecological research on aquatic beetles from the genus <i>Ochthebius</i> (Coleoptera, Hydraenidae). Some of these non-model species, with high cryptic diversity, exclusively inhabit supratidal rockpools, extreme and highly dynamic habitats with important anthropogenic threats. We analysed 15 individuals of four species (<i>O. lejolisii</i>, <i>O. subinteger</i>, <i>O. celatus</i>, and <i>O. quadricollis</i>) across 10 localities from the Mediterranean coasts of Spain and Malta. Using next-generation sequencing technology, two libraries were constructed to interpret the species of the two subgenera present consistently (<i>Ochthebius</i> s. str., <i>O. quadricollis</i>; and <i>Cobalius</i>, the rest of the species). Finally, 20 markers (10 for each subgenus) were obtained and successfully tested by cross-validation in the four species under study. As a by-catch, we could retrieve the complete mitochondrial genomes of <i>O. lejolisii</i>, <i>O. quadricollis</i>, and <i>O. subinteger</i>. Interestingly, the mitochondrial genome of <i>O. quadricollis</i> exhibited high genetic variability compared to already published data. The novel SSR panels and mitochondrial genomes for <i>Ochthebius</i> will be valuable in future research on species identification, diversity, genetic structure, and population connectivity in highly dynamic and threatened habitats such as supratidal coastal rockpools.https://www.mdpi.com/2075-4450/14/11/881water beetlesmicrosatellite markersnext-generation sequencingcross-species validationinsect conservation |
| spellingShingle | Antonio José García-Meseguer Adrián Villastrigo Juana María Mirón-Gatón Andrés Millán Josefa Velasco Irene Muñoz Novel Microsatellite Loci, Cross-Species Validation of Multiplex Assays, and By-Catch Mitochondrial Genomes on <i>Ochthebius</i> Beetles from Supratidal Rockpools Insects water beetles microsatellite markers next-generation sequencing cross-species validation insect conservation |
| title | Novel Microsatellite Loci, Cross-Species Validation of Multiplex Assays, and By-Catch Mitochondrial Genomes on <i>Ochthebius</i> Beetles from Supratidal Rockpools |
| title_full | Novel Microsatellite Loci, Cross-Species Validation of Multiplex Assays, and By-Catch Mitochondrial Genomes on <i>Ochthebius</i> Beetles from Supratidal Rockpools |
| title_fullStr | Novel Microsatellite Loci, Cross-Species Validation of Multiplex Assays, and By-Catch Mitochondrial Genomes on <i>Ochthebius</i> Beetles from Supratidal Rockpools |
| title_full_unstemmed | Novel Microsatellite Loci, Cross-Species Validation of Multiplex Assays, and By-Catch Mitochondrial Genomes on <i>Ochthebius</i> Beetles from Supratidal Rockpools |
| title_short | Novel Microsatellite Loci, Cross-Species Validation of Multiplex Assays, and By-Catch Mitochondrial Genomes on <i>Ochthebius</i> Beetles from Supratidal Rockpools |
| title_sort | novel microsatellite loci cross species validation of multiplex assays and by catch mitochondrial genomes on i ochthebius i beetles from supratidal rockpools |
| topic | water beetles microsatellite markers next-generation sequencing cross-species validation insect conservation |
| url | https://www.mdpi.com/2075-4450/14/11/881 |
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