Morphological examination of pelvic floor muscles in a rat model of vaginal delivery
Abstract Objective This study investigated morphological changes in the composition of the pelvic floor muscles, degree of atrophy, and urethral function in a rat of simulated birth trauma induced by vaginal distension (VD) model. Methods Female Sprague–Dawley rats were classified into four groups:...
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| Format: | Article |
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BMC
2024-01-01
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| Series: | BMC Pregnancy and Childbirth |
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| Online Access: | https://doi.org/10.1186/s12884-024-06278-5 |
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| author | Yui Abe-Takahashi Takeya Kitta Mifuka Ouchi Hiroki Chiba Madoka Higuchi Mio Togo Naohisa Kusakabe Hidehiro Kakizaki Nobuo Shinohara |
| author_facet | Yui Abe-Takahashi Takeya Kitta Mifuka Ouchi Hiroki Chiba Madoka Higuchi Mio Togo Naohisa Kusakabe Hidehiro Kakizaki Nobuo Shinohara |
| author_sort | Yui Abe-Takahashi |
| collection | DOAJ |
| description | Abstract Objective This study investigated morphological changes in the composition of the pelvic floor muscles, degree of atrophy, and urethral function in a rat of simulated birth trauma induced by vaginal distension (VD) model. Methods Female Sprague–Dawley rats were classified into four groups: a sham group, and 1, 2, and 4 weeks post-VD (1 W, 2 W, and 4 W, respectively) groups. We measured the amplitude of urethral response to electrical stimulation (A-URE) to evaluate urethral function. After measuring the muscle wet weight of the pubococcygeus (Pcm) and iliococcygeus (Icm) muscles, histochemical staining was used to classify muscle fibers into Types I, IIa, and IIb, and the occupancy and cross-sectional area of each muscle fiber were determined. Results There were 24 Sprague–Dawley rats used. A-URE was significantly lower in the 1 W group versus the other groups. Muscle wet weight was significantly lower in the VD groups versus the sham group for Pcm. The cross-sectional area of Type I Pcm and Icm was significantly lower in the VD groups versus the sham group. Type I muscle fiber composition in Pcm was significantly lower in the VD groups versus the sham groupand lowest in the 2 W group. Type I muscle fiber composition in Icm was significantly lower in the 2 and 4 W groups versus the sham group. Conclusion Muscle atrophy and changes in muscle composition in the pelvic floor muscles were observed even after improvements in urethral function. These results may provide insight into the pathogenesis of stress urinary incontinence after VD. |
| first_indexed | 2024-03-07T14:36:00Z |
| format | Article |
| id | doaj.art-84f33a52a2e349c08e707ac2503580de |
| institution | Directory Open Access Journal |
| issn | 1471-2393 |
| language | English |
| last_indexed | 2024-03-07T14:36:00Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pregnancy and Childbirth |
| spelling | doaj.art-84f33a52a2e349c08e707ac2503580de2024-03-05T20:40:17ZengBMCBMC Pregnancy and Childbirth1471-23932024-01-012411710.1186/s12884-024-06278-5Morphological examination of pelvic floor muscles in a rat model of vaginal deliveryYui Abe-Takahashi0Takeya Kitta1Mifuka Ouchi2Hiroki Chiba3Madoka Higuchi4Mio Togo5Naohisa Kusakabe6Hidehiro Kakizaki7Nobuo Shinohara8Department of Renal and Genitourinary Surgery, Graduate School of Medicine, Hokkaido UniversityDepartment of Renal and Urologic Surgery, Asahikawa Medical UniversityDepartment of Renal and Genitourinary Surgery, Graduate School of Medicine, Hokkaido UniversityDepartment of Renal and Genitourinary Surgery, Graduate School of Medicine, Hokkaido UniversityDepartment of Renal and Genitourinary Surgery, Graduate School of Medicine, Hokkaido UniversityDepartment of Renal and Genitourinary Surgery, Graduate School of Medicine, Hokkaido UniversityDepartment of Renal and Genitourinary Surgery, Graduate School of Medicine, Hokkaido UniversityDepartment of Renal and Urologic Surgery, Asahikawa Medical UniversityDepartment of Renal and Genitourinary Surgery, Graduate School of Medicine, Hokkaido UniversityAbstract Objective This study investigated morphological changes in the composition of the pelvic floor muscles, degree of atrophy, and urethral function in a rat of simulated birth trauma induced by vaginal distension (VD) model. Methods Female Sprague–Dawley rats were classified into four groups: a sham group, and 1, 2, and 4 weeks post-VD (1 W, 2 W, and 4 W, respectively) groups. We measured the amplitude of urethral response to electrical stimulation (A-URE) to evaluate urethral function. After measuring the muscle wet weight of the pubococcygeus (Pcm) and iliococcygeus (Icm) muscles, histochemical staining was used to classify muscle fibers into Types I, IIa, and IIb, and the occupancy and cross-sectional area of each muscle fiber were determined. Results There were 24 Sprague–Dawley rats used. A-URE was significantly lower in the 1 W group versus the other groups. Muscle wet weight was significantly lower in the VD groups versus the sham group for Pcm. The cross-sectional area of Type I Pcm and Icm was significantly lower in the VD groups versus the sham group. Type I muscle fiber composition in Pcm was significantly lower in the VD groups versus the sham groupand lowest in the 2 W group. Type I muscle fiber composition in Icm was significantly lower in the 2 and 4 W groups versus the sham group. Conclusion Muscle atrophy and changes in muscle composition in the pelvic floor muscles were observed even after improvements in urethral function. These results may provide insight into the pathogenesis of stress urinary incontinence after VD.https://doi.org/10.1186/s12884-024-06278-5Morphological changeMuscle fiber compositionPelvic floor musclesStress urinary incontinenceUrethral functionVaginal distention |
| spellingShingle | Yui Abe-Takahashi Takeya Kitta Mifuka Ouchi Hiroki Chiba Madoka Higuchi Mio Togo Naohisa Kusakabe Hidehiro Kakizaki Nobuo Shinohara Morphological examination of pelvic floor muscles in a rat model of vaginal delivery BMC Pregnancy and Childbirth Morphological change Muscle fiber composition Pelvic floor muscles Stress urinary incontinence Urethral function Vaginal distention |
| title | Morphological examination of pelvic floor muscles in a rat model of vaginal delivery |
| title_full | Morphological examination of pelvic floor muscles in a rat model of vaginal delivery |
| title_fullStr | Morphological examination of pelvic floor muscles in a rat model of vaginal delivery |
| title_full_unstemmed | Morphological examination of pelvic floor muscles in a rat model of vaginal delivery |
| title_short | Morphological examination of pelvic floor muscles in a rat model of vaginal delivery |
| title_sort | morphological examination of pelvic floor muscles in a rat model of vaginal delivery |
| topic | Morphological change Muscle fiber composition Pelvic floor muscles Stress urinary incontinence Urethral function Vaginal distention |
| url | https://doi.org/10.1186/s12884-024-06278-5 |
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