Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with <i>Citrullus colocynthis</i>
This study was conducted to investigate the antagonistic potential of endophytic and rhizospheric bacterial isolates obtained from <i>Citrullus colocynthis</i> in suppressing <i>Fusarium solani</i> and <i>Pythium aphanidermatum</i> and promoting the growth of cucu...
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MDPI AG
2023-10-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/11/1275 |
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| author | Badriya Khalfan Al-Shuaibi Elham Ahmed Kazerooni Shah Hussain Rethinasamy Velazhahan Abdullah Mohammed Al-Sadi |
| author_facet | Badriya Khalfan Al-Shuaibi Elham Ahmed Kazerooni Shah Hussain Rethinasamy Velazhahan Abdullah Mohammed Al-Sadi |
| author_sort | Badriya Khalfan Al-Shuaibi |
| collection | DOAJ |
| description | This study was conducted to investigate the antagonistic potential of endophytic and rhizospheric bacterial isolates obtained from <i>Citrullus colocynthis</i> in suppressing <i>Fusarium solani</i> and <i>Pythium aphanidermatum</i> and promoting the growth of cucumber. Molecular identification of bacterial strains associated with <i>C. colocynthis</i> confirmed that these strains belong to the <i>Achromobacter</i>, <i>Pantoea</i>, <i>Pseudomonas</i>, <i>Rhizobium</i>, <i>Sphingobacterium</i>, <i>Bacillus</i>, <i>Sinorhizobium</i>, <i>Staphylococcus</i>, <i>Cupriavidus</i>, and <i>Exiguobacterium</i> genera. A dual culture assay showed that nine of the bacterial strains exhibited antifungal activity, four of which were effective against both pathogens. Strains B27 (<i>Pantoea dispersa</i>) and B28 (<i>Exiguobacterium indicum</i>) caused the highest percentage of inhibition towards <i>F. solani</i> (48.5% and 48.1%, respectively). <i>P. aphanidermatum</i> growth was impeded by the B21 (<i>Bacillus cereus</i>, 44.7%) and B28 (<i>Exiguobacterium indicum</i>, 51.1%) strains. Scanning electron microscopy showed that the strains caused abnormality in phytopathogens’ mycelia. All of the selected bacterial strains showed good IAA production (>500 ppm). A paper towel experiment demonstrated that these strains improved the seed germination, root/shoot growth, and vigor index of cucumber seedlings. Our findings suggest that the bacterial strains from <i>C. colocynthis</i> are suppressive to <i>F. solani</i> and <i>P. aphanidermatum</i> and can promote cucumber growth. This appears to be the first study to report the efficacy of these bacterial strains from <i>C. colocynthis</i> against <i>F. solani</i> and <i>P. aphanidermatum</i>. |
| first_indexed | 2024-03-09T16:33:25Z |
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| id | doaj.art-84f4e46fa927461da532ad3a276e3e70 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
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| last_indexed | 2024-03-09T16:33:25Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-84f4e46fa927461da532ad3a276e3e702023-11-24T14:59:34ZengMDPI AGPathogens2076-08172023-10-011211127510.3390/pathogens12111275Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with <i>Citrullus colocynthis</i>Badriya Khalfan Al-Shuaibi0Elham Ahmed Kazerooni1Shah Hussain2Rethinasamy Velazhahan3Abdullah Mohammed Al-Sadi4Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, P.O. Box 34, Al-Khod 123, OmanDepartment of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, P.O. Box 34, Al-Khod 123, OmanDepartment of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, P.O. Box 34, Al-Khod 123, OmanDepartment of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, P.O. Box 34, Al-Khod 123, OmanDepartment of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, P.O. Box 34, Al-Khod 123, OmanThis study was conducted to investigate the antagonistic potential of endophytic and rhizospheric bacterial isolates obtained from <i>Citrullus colocynthis</i> in suppressing <i>Fusarium solani</i> and <i>Pythium aphanidermatum</i> and promoting the growth of cucumber. Molecular identification of bacterial strains associated with <i>C. colocynthis</i> confirmed that these strains belong to the <i>Achromobacter</i>, <i>Pantoea</i>, <i>Pseudomonas</i>, <i>Rhizobium</i>, <i>Sphingobacterium</i>, <i>Bacillus</i>, <i>Sinorhizobium</i>, <i>Staphylococcus</i>, <i>Cupriavidus</i>, and <i>Exiguobacterium</i> genera. A dual culture assay showed that nine of the bacterial strains exhibited antifungal activity, four of which were effective against both pathogens. Strains B27 (<i>Pantoea dispersa</i>) and B28 (<i>Exiguobacterium indicum</i>) caused the highest percentage of inhibition towards <i>F. solani</i> (48.5% and 48.1%, respectively). <i>P. aphanidermatum</i> growth was impeded by the B21 (<i>Bacillus cereus</i>, 44.7%) and B28 (<i>Exiguobacterium indicum</i>, 51.1%) strains. Scanning electron microscopy showed that the strains caused abnormality in phytopathogens’ mycelia. All of the selected bacterial strains showed good IAA production (>500 ppm). A paper towel experiment demonstrated that these strains improved the seed germination, root/shoot growth, and vigor index of cucumber seedlings. Our findings suggest that the bacterial strains from <i>C. colocynthis</i> are suppressive to <i>F. solani</i> and <i>P. aphanidermatum</i> and can promote cucumber growth. This appears to be the first study to report the efficacy of these bacterial strains from <i>C. colocynthis</i> against <i>F. solani</i> and <i>P. aphanidermatum</i>.https://www.mdpi.com/2076-0817/12/11/1275antagonistic bacteriabiological controldamping-off<i>Fusarium solani</i>plant growth promotion<i>Pythium aphanidermatum</i> |
| spellingShingle | Badriya Khalfan Al-Shuaibi Elham Ahmed Kazerooni Shah Hussain Rethinasamy Velazhahan Abdullah Mohammed Al-Sadi Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with <i>Citrullus colocynthis</i> Pathogens antagonistic bacteria biological control damping-off <i>Fusarium solani</i> plant growth promotion <i>Pythium aphanidermatum</i> |
| title | Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with <i>Citrullus colocynthis</i> |
| title_full | Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with <i>Citrullus colocynthis</i> |
| title_fullStr | Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with <i>Citrullus colocynthis</i> |
| title_full_unstemmed | Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with <i>Citrullus colocynthis</i> |
| title_short | Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with <i>Citrullus colocynthis</i> |
| title_sort | plant disease suppressive and growth promoting activities of endophytic and rhizobacterial isolates associated with i citrullus colocynthis i |
| topic | antagonistic bacteria biological control damping-off <i>Fusarium solani</i> plant growth promotion <i>Pythium aphanidermatum</i> |
| url | https://www.mdpi.com/2076-0817/12/11/1275 |
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