In-vivo inhibition of latanoprost induced iridal hyperpigmentation in rabbits- An investigational study
Objectives: To evaluate the inhibitory effects of different concentrations of α-methyl-DL-tyrosine on latanoprost-induced iridal hyperpigmentation in rabbits. Methods: We investigated 4 groups of rabbits. Both eyes of the pink, red, and blue groups were treated with latanoprost followed by 0.5%, 1%,...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022027736 |
| _version_ | 1811213625332858880 |
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| author | Muhammad Sadiq Waqar Ahmad Muhammad Bilal Mahmood Ali Mir Azam Khan Farah Akhtar |
| author_facet | Muhammad Sadiq Waqar Ahmad Muhammad Bilal Mahmood Ali Mir Azam Khan Farah Akhtar |
| author_sort | Muhammad Sadiq |
| collection | DOAJ |
| description | Objectives: To evaluate the inhibitory effects of different concentrations of α-methyl-DL-tyrosine on latanoprost-induced iridal hyperpigmentation in rabbits. Methods: We investigated 4 groups of rabbits. Both eyes of the pink, red, and blue groups were treated with latanoprost followed by 0.5%, 1%, and 2% α-methyl-DL-tyrosine (inhibitor) in the right eyes respectively and the green group received only inhibitor. We prospectively investigated the irides, estimated quantitatively total melanin contents, and studied any histopathological changes that occurred. Results: The observers favored hyperpigmentation in the left eyes while in the right eyes they noted a decrease in pigmentation as compared to the baseline. An increase in pigmentation was noted by 93.33% of observers in the left eye of the blue group. A significant difference in the mean melanin contents was noted in the blue group (Right eye = 09.560 μg/g (±0.750), Left eye = 3.730 μg/g (±1.062). There was no evidence of stromal malignant changes, Hemorrhage, mitosis, inflammation, and atypical melanocytes in all specimens. A moderate degree of pigmentation in the left eye of the red group was noted. Mild stromal-free melanin pigment was present in all samples of pink, red and blue groups. Conclusions: The α-methyl-DL-tyrosine significantly inhibited latanoprost-induced iridal pigmentation without causing any histopathological changes at a 2% dose. |
| first_indexed | 2024-04-12T05:49:46Z |
| format | Article |
| id | doaj.art-84f7e9724267461293c39f0b095f2efa |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-12T05:49:46Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-84f7e9724267461293c39f0b095f2efa2022-12-22T03:45:20ZengElsevierHeliyon2405-84402022-11-01811e11485In-vivo inhibition of latanoprost induced iridal hyperpigmentation in rabbits- An investigational studyMuhammad Sadiq0Waqar Ahmad1Muhammad Bilal2Mahmood Ali3Mir Azam Khan4Farah Akhtar5Department of Pharmacy, University of Malakand, Chakdara, PakistanDepartment of Pharmacy, Bacha Khan University, Charsadda, Pakistan; Corresponding author.Pathology Department, Shifa International Hospital, Islamabad, PakistanGlaucoma Department, Al-Shifa Trust Eye Hospital, Rawalpindi, PakistanDepartment of Pharmacy, University of Malakand, Chakdara, PakistanGlaucoma Department, Al-Shifa Trust Eye Hospital, Rawalpindi, PakistanObjectives: To evaluate the inhibitory effects of different concentrations of α-methyl-DL-tyrosine on latanoprost-induced iridal hyperpigmentation in rabbits. Methods: We investigated 4 groups of rabbits. Both eyes of the pink, red, and blue groups were treated with latanoprost followed by 0.5%, 1%, and 2% α-methyl-DL-tyrosine (inhibitor) in the right eyes respectively and the green group received only inhibitor. We prospectively investigated the irides, estimated quantitatively total melanin contents, and studied any histopathological changes that occurred. Results: The observers favored hyperpigmentation in the left eyes while in the right eyes they noted a decrease in pigmentation as compared to the baseline. An increase in pigmentation was noted by 93.33% of observers in the left eye of the blue group. A significant difference in the mean melanin contents was noted in the blue group (Right eye = 09.560 μg/g (±0.750), Left eye = 3.730 μg/g (±1.062). There was no evidence of stromal malignant changes, Hemorrhage, mitosis, inflammation, and atypical melanocytes in all specimens. A moderate degree of pigmentation in the left eye of the red group was noted. Mild stromal-free melanin pigment was present in all samples of pink, red and blue groups. Conclusions: The α-methyl-DL-tyrosine significantly inhibited latanoprost-induced iridal pigmentation without causing any histopathological changes at a 2% dose.http://www.sciencedirect.com/science/article/pii/S2405844022027736Latanoprostα-methyl-DL-tyrosineHyperpigmentationInhibitionPakistan |
| spellingShingle | Muhammad Sadiq Waqar Ahmad Muhammad Bilal Mahmood Ali Mir Azam Khan Farah Akhtar In-vivo inhibition of latanoprost induced iridal hyperpigmentation in rabbits- An investigational study Heliyon Latanoprost α-methyl-DL-tyrosine Hyperpigmentation Inhibition Pakistan |
| title | In-vivo inhibition of latanoprost induced iridal hyperpigmentation in rabbits- An investigational study |
| title_full | In-vivo inhibition of latanoprost induced iridal hyperpigmentation in rabbits- An investigational study |
| title_fullStr | In-vivo inhibition of latanoprost induced iridal hyperpigmentation in rabbits- An investigational study |
| title_full_unstemmed | In-vivo inhibition of latanoprost induced iridal hyperpigmentation in rabbits- An investigational study |
| title_short | In-vivo inhibition of latanoprost induced iridal hyperpigmentation in rabbits- An investigational study |
| title_sort | in vivo inhibition of latanoprost induced iridal hyperpigmentation in rabbits an investigational study |
| topic | Latanoprost α-methyl-DL-tyrosine Hyperpigmentation Inhibition Pakistan |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022027736 |
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