Automated high-throughput DNA synthesis and assembly
DNA synthesis and assembly primarily revolve around the innovation and refinement of tools that facilitate the creation of specific genes and the manipulation of entire genomes. This multifaceted process encompasses two fundamental steps: the synthesis of lengthy oligonucleotides and the seamless as...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024029980 |
| _version_ | 1827294075692253184 |
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| author | Yuxin Ma Zhaoyang Zhang Bin Jia Yingjin Yuan |
| author_facet | Yuxin Ma Zhaoyang Zhang Bin Jia Yingjin Yuan |
| author_sort | Yuxin Ma |
| collection | DOAJ |
| description | DNA synthesis and assembly primarily revolve around the innovation and refinement of tools that facilitate the creation of specific genes and the manipulation of entire genomes. This multifaceted process encompasses two fundamental steps: the synthesis of lengthy oligonucleotides and the seamless assembly of numerous DNA fragments. With the advent of automated pipetting workstations and integrated experimental equipment, a substantial portion of repetitive tasks in the field of synthetic biology can now be efficiently accomplished through integrated liquid handling workstations. This not only reduces the need for manual labor but also enhances overall efficiency. This review explores the ongoing advancements in the oligonucleotide synthesis platform, automated DNA assembly techniques, and biofoundries. The development of accurate and high-throughput DNA synthesis and assembly technologies presents both challenges and opportunities. |
| first_indexed | 2024-04-24T13:50:32Z |
| format | Article |
| id | doaj.art-84f80eb7855544ee9eaf2a23111788be |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:50:32Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-84f80eb7855544ee9eaf2a23111788be2024-04-04T05:04:19ZengElsevierHeliyon2405-84402024-03-01106e26967Automated high-throughput DNA synthesis and assemblyYuxin Ma0Zhaoyang Zhang1Bin Jia2Yingjin Yuan3Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, ChinaFrontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, ChinaCorresponding author.; Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, ChinaFrontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, ChinaDNA synthesis and assembly primarily revolve around the innovation and refinement of tools that facilitate the creation of specific genes and the manipulation of entire genomes. This multifaceted process encompasses two fundamental steps: the synthesis of lengthy oligonucleotides and the seamless assembly of numerous DNA fragments. With the advent of automated pipetting workstations and integrated experimental equipment, a substantial portion of repetitive tasks in the field of synthetic biology can now be efficiently accomplished through integrated liquid handling workstations. This not only reduces the need for manual labor but also enhances overall efficiency. This review explores the ongoing advancements in the oligonucleotide synthesis platform, automated DNA assembly techniques, and biofoundries. The development of accurate and high-throughput DNA synthesis and assembly technologies presents both challenges and opportunities.http://www.sciencedirect.com/science/article/pii/S2405844024029980DNA synthesisMicroarray oligonucleotide synthesisEnzymatic oligonucleotide synthesisAutomated DNA assemblySynthetic biology |
| spellingShingle | Yuxin Ma Zhaoyang Zhang Bin Jia Yingjin Yuan Automated high-throughput DNA synthesis and assembly Heliyon DNA synthesis Microarray oligonucleotide synthesis Enzymatic oligonucleotide synthesis Automated DNA assembly Synthetic biology |
| title | Automated high-throughput DNA synthesis and assembly |
| title_full | Automated high-throughput DNA synthesis and assembly |
| title_fullStr | Automated high-throughput DNA synthesis and assembly |
| title_full_unstemmed | Automated high-throughput DNA synthesis and assembly |
| title_short | Automated high-throughput DNA synthesis and assembly |
| title_sort | automated high throughput dna synthesis and assembly |
| topic | DNA synthesis Microarray oligonucleotide synthesis Enzymatic oligonucleotide synthesis Automated DNA assembly Synthetic biology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024029980 |
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