Study of the Repellent Activity of 60 Essential Oils and Their Main Constituents against <i>Aedes albopictus</i>, and Nano-Formulation Development
Mosquitoes are one of the most important disease vectors from a medical viewpoint in that they transmit several diseases such as malaria, filariasis, yellow and Dengue fever. Mosquito vector control and personal protection from mosquito bites are currently the most efficient ways to prevent these di...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/12/1077 |
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| author | Weifeng Wu Yu Yang Yingmiao Feng Xiaofei Ren Yuling Li Wenjiao Li Jietong Huang Lingjia Kong Xiaole Chen Zhongze Lin Xiaohui Hou Longlai Zhang Yajie Chen Zhaojun Sheng Weiqian Hong |
| author_facet | Weifeng Wu Yu Yang Yingmiao Feng Xiaofei Ren Yuling Li Wenjiao Li Jietong Huang Lingjia Kong Xiaole Chen Zhongze Lin Xiaohui Hou Longlai Zhang Yajie Chen Zhaojun Sheng Weiqian Hong |
| author_sort | Weifeng Wu |
| collection | DOAJ |
| description | Mosquitoes are one of the most important disease vectors from a medical viewpoint in that they transmit several diseases such as malaria, filariasis, yellow and Dengue fever. Mosquito vector control and personal protection from mosquito bites are currently the most efficient ways to prevent these diseases. Several synthetic repellents such as DEET, ethyl butylacetylaminopropionate (IR3535) and 1-(1-methylpropoxycarbonyl)-2-(2-hydroxyethyl)piperidine) (Picaridin), have been widely used to prevent humans from receiving mosquito bites. However, the use of synthetic repellents has raised several environment and health concerns. Therefore, essential oils (EOs) as natural alternatives receive our attention. In order to discover highly effective mosquito repellents from natural sources, the repellent activity of 60 commercial EOs against <i>Ae. albopictus</i> was screened in this study. Eight EOs including cinnamon, marjoram, lemongrass, bay, chamomile, jasmine, peppermint2, and thyme, showed a suitable repellent rate (>40%) at the tested dose of 10 μg/cm<sup>2</sup>. Then, their main constituents were analyzed by GC-MS, and the active constituents were identified. The most active compounds including cinnamaldehyde, citral and terpinen-4-ol, exhibited an 82%, 65% and 60% repellent rate, respectively. Moreover, the nanoemulsions of the three active compounds were prepared and characterized. In the arm-in-cage assay, the protection times of the nanoemulsions of cinnamaldehyde and citral were significantly extended compared with their normal solutions. This study provides several lead compounds to develop new mosquito repellents, and it suggests that nanoemulsification is an effective method for improving the duration of the activity of natural repellents. |
| first_indexed | 2024-03-09T16:17:51Z |
| format | Article |
| id | doaj.art-84fb8de3ad1545daacaef149a255a588 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T16:17:51Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-84fb8de3ad1545daacaef149a255a5882023-11-24T15:38:45ZengMDPI AGInsects2075-44502022-11-011312107710.3390/insects13121077Study of the Repellent Activity of 60 Essential Oils and Their Main Constituents against <i>Aedes albopictus</i>, and Nano-Formulation DevelopmentWeifeng Wu0Yu Yang1Yingmiao Feng2Xiaofei Ren3Yuling Li4Wenjiao Li5Jietong Huang6Lingjia Kong7Xiaole Chen8Zhongze Lin9Xiaohui Hou10Longlai Zhang11Yajie Chen12Zhaojun Sheng13Weiqian Hong14School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaThe Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaFaculty of Southern Medicine, Guangdong Jiangmen Chinese Medicine College, Jiangmen 529000, ChinaSchool of Preclinical Medicine, Zunyi Medical University, Zunyi 563003, ChinaMHOME (Guangzhou) Industrial Co., Ltd., Guangzhou 510700, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529020, ChinaMosquitoes are one of the most important disease vectors from a medical viewpoint in that they transmit several diseases such as malaria, filariasis, yellow and Dengue fever. Mosquito vector control and personal protection from mosquito bites are currently the most efficient ways to prevent these diseases. Several synthetic repellents such as DEET, ethyl butylacetylaminopropionate (IR3535) and 1-(1-methylpropoxycarbonyl)-2-(2-hydroxyethyl)piperidine) (Picaridin), have been widely used to prevent humans from receiving mosquito bites. However, the use of synthetic repellents has raised several environment and health concerns. Therefore, essential oils (EOs) as natural alternatives receive our attention. In order to discover highly effective mosquito repellents from natural sources, the repellent activity of 60 commercial EOs against <i>Ae. albopictus</i> was screened in this study. Eight EOs including cinnamon, marjoram, lemongrass, bay, chamomile, jasmine, peppermint2, and thyme, showed a suitable repellent rate (>40%) at the tested dose of 10 μg/cm<sup>2</sup>. Then, their main constituents were analyzed by GC-MS, and the active constituents were identified. The most active compounds including cinnamaldehyde, citral and terpinen-4-ol, exhibited an 82%, 65% and 60% repellent rate, respectively. Moreover, the nanoemulsions of the three active compounds were prepared and characterized. In the arm-in-cage assay, the protection times of the nanoemulsions of cinnamaldehyde and citral were significantly extended compared with their normal solutions. This study provides several lead compounds to develop new mosquito repellents, and it suggests that nanoemulsification is an effective method for improving the duration of the activity of natural repellents.https://www.mdpi.com/2075-4450/13/12/1077essential oilrepellent<i>Aedes albopictus</i>nanoemulsion |
| spellingShingle | Weifeng Wu Yu Yang Yingmiao Feng Xiaofei Ren Yuling Li Wenjiao Li Jietong Huang Lingjia Kong Xiaole Chen Zhongze Lin Xiaohui Hou Longlai Zhang Yajie Chen Zhaojun Sheng Weiqian Hong Study of the Repellent Activity of 60 Essential Oils and Their Main Constituents against <i>Aedes albopictus</i>, and Nano-Formulation Development Insects essential oil repellent <i>Aedes albopictus</i> nanoemulsion |
| title | Study of the Repellent Activity of 60 Essential Oils and Their Main Constituents against <i>Aedes albopictus</i>, and Nano-Formulation Development |
| title_full | Study of the Repellent Activity of 60 Essential Oils and Their Main Constituents against <i>Aedes albopictus</i>, and Nano-Formulation Development |
| title_fullStr | Study of the Repellent Activity of 60 Essential Oils and Their Main Constituents against <i>Aedes albopictus</i>, and Nano-Formulation Development |
| title_full_unstemmed | Study of the Repellent Activity of 60 Essential Oils and Their Main Constituents against <i>Aedes albopictus</i>, and Nano-Formulation Development |
| title_short | Study of the Repellent Activity of 60 Essential Oils and Their Main Constituents against <i>Aedes albopictus</i>, and Nano-Formulation Development |
| title_sort | study of the repellent activity of 60 essential oils and their main constituents against i aedes albopictus i and nano formulation development |
| topic | essential oil repellent <i>Aedes albopictus</i> nanoemulsion |
| url | https://www.mdpi.com/2075-4450/13/12/1077 |
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