DNA methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adults
Obesity is a major risk factor for cardiovascular disease. Blood-detected epigenetic profiles may serve as non-invasive clinically relevant biomarkers. Therefore, we investigated DNA methylation of genes involved in inflammation in peripheral blood of obese subjects and lean controls and their corre...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-01-01
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| Series: | Epigenetics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592294.2021.1876285 |
| _version_ | 1827811474449367040 |
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| author | Mohamed M. Ali Dina Naquiallah Maryam Qureshi Mohammed Imaduddin Mirza Chandra Hassan Mario Masrur Francesco M. Bianco Patrice Frederick Giulianotti P. Cristoforo Antonio Gangemi Shane A. Phillips Abeer M. Mahmoud |
| author_facet | Mohamed M. Ali Dina Naquiallah Maryam Qureshi Mohammed Imaduddin Mirza Chandra Hassan Mario Masrur Francesco M. Bianco Patrice Frederick Giulianotti P. Cristoforo Antonio Gangemi Shane A. Phillips Abeer M. Mahmoud |
| author_sort | Mohamed M. Ali |
| collection | DOAJ |
| description | Obesity is a major risk factor for cardiovascular disease. Blood-detected epigenetic profiles may serve as non-invasive clinically relevant biomarkers. Therefore, we investigated DNA methylation of genes involved in inflammation in peripheral blood of obese subjects and lean controls and their correlation with cardiometabolic measurements. We obtained blood and adipose tissue (AT) samples from bariatric patients (n = 24) and control adults (n = 24). AT-isolated arterioles were tested for flow-induced dilation (FID) and production of nitric oxide (NO) and reactive oxygen species (ROS). Brachial artery flow-mediated dilation (FMD) was measured via doppler ultrasound. Promoter methylation of 94 genes involved in inflammation and autoimmunity were analysed in whole-blood DNA in relation to vascular function and cardiometabolic risk factors. 77 genes had ahigher methylated fraction in the controls compare obese subjects and 28 proinflammatory genes were significantly hypomethylated in the obese individuals; on top of these genes are CXCL1, CXCL12, CXCL6, IGF2BP2, HDAC4, IL12A, and IL17RA. Fifteen of these genes had significantly higher mRNA in obese subjects compared to controls; on top of these genes are CXCL6, TLR5, IL6ST, EGR1, IL15RA, and HDAC4. Methylation % inversely correlated with BMI, total fat %, visceral fat%, blood pressure, fasting plasma insulin, serum IL6 and C-reactive protein, arteriolar ROS, and alcohol consumption and positive correlations with lean %, HDL, plasma folate and vitamin B12, arteriolar FID and NO production, and brachial FMD. Our results suggest that vascular dysfunction in obese adults may be attributed to asystemic hypomethylation and over expression of the immune-related genes. |
| first_indexed | 2024-03-11T23:05:37Z |
| format | Article |
| id | doaj.art-8502b0c6ff05487a8952fd5a0e714685 |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:05:37Z |
| publishDate | 2022-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-8502b0c6ff05487a8952fd5a0e7146852023-09-21T13:09:25ZengTaylor & Francis GroupEpigenetics1559-22941559-23082022-01-011719310910.1080/15592294.2021.18762851876285DNA methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adultsMohamed M. Ali0Dina Naquiallah1Maryam Qureshi2Mohammed Imaduddin Mirza3Chandra Hassan4Mario Masrur5Francesco M. Bianco6Patrice Frederick7Giulianotti P. Cristoforo8Antonio Gangemi9Shane A. Phillips10Abeer M. Mahmoud11College of Applied Health Sciences, University of Illinois at ChicagoCollege of Medicine, University of Illinois at ChicagoCollege of Medicine, University of Illinois at ChicagoCollege of Medicine, University of Illinois at ChicagoUniversity of Illinois at ChicagoUniversity of Illinois at ChicagoUniversity of Illinois at ChicagoUniversity of Illinois at ChicagoUniversity of Illinois at ChicagoUniversity of Illinois at ChicagoCollege of Applied Health Sciences, University of Illinois at ChicagoCollege of Medicine, University of Illinois at ChicagoObesity is a major risk factor for cardiovascular disease. Blood-detected epigenetic profiles may serve as non-invasive clinically relevant biomarkers. Therefore, we investigated DNA methylation of genes involved in inflammation in peripheral blood of obese subjects and lean controls and their correlation with cardiometabolic measurements. We obtained blood and adipose tissue (AT) samples from bariatric patients (n = 24) and control adults (n = 24). AT-isolated arterioles were tested for flow-induced dilation (FID) and production of nitric oxide (NO) and reactive oxygen species (ROS). Brachial artery flow-mediated dilation (FMD) was measured via doppler ultrasound. Promoter methylation of 94 genes involved in inflammation and autoimmunity were analysed in whole-blood DNA in relation to vascular function and cardiometabolic risk factors. 77 genes had ahigher methylated fraction in the controls compare obese subjects and 28 proinflammatory genes were significantly hypomethylated in the obese individuals; on top of these genes are CXCL1, CXCL12, CXCL6, IGF2BP2, HDAC4, IL12A, and IL17RA. Fifteen of these genes had significantly higher mRNA in obese subjects compared to controls; on top of these genes are CXCL6, TLR5, IL6ST, EGR1, IL15RA, and HDAC4. Methylation % inversely correlated with BMI, total fat %, visceral fat%, blood pressure, fasting plasma insulin, serum IL6 and C-reactive protein, arteriolar ROS, and alcohol consumption and positive correlations with lean %, HDL, plasma folate and vitamin B12, arteriolar FID and NO production, and brachial FMD. Our results suggest that vascular dysfunction in obese adults may be attributed to asystemic hypomethylation and over expression of the immune-related genes.http://dx.doi.org/10.1080/15592294.2021.1876285dna methylationobesityinflammationvascular functionflow-induced dilationcardiometabolic risk |
| spellingShingle | Mohamed M. Ali Dina Naquiallah Maryam Qureshi Mohammed Imaduddin Mirza Chandra Hassan Mario Masrur Francesco M. Bianco Patrice Frederick Giulianotti P. Cristoforo Antonio Gangemi Shane A. Phillips Abeer M. Mahmoud DNA methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adults Epigenetics dna methylation obesity inflammation vascular function flow-induced dilation cardiometabolic risk |
| title | DNA methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adults |
| title_full | DNA methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adults |
| title_fullStr | DNA methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adults |
| title_full_unstemmed | DNA methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adults |
| title_short | DNA methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adults |
| title_sort | dna methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adults |
| topic | dna methylation obesity inflammation vascular function flow-induced dilation cardiometabolic risk |
| url | http://dx.doi.org/10.1080/15592294.2021.1876285 |
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