Metabolomic profiling identifies biomarkers and metabolic impacts of surgery for colorectal cancer
BackgroundColorectal cancer (CRC) is one of the most common malignant tumors with recurrence and metastasis after surgical resection. This study aimed to identify the physiological changes after surgery and explore metabolites and metabolic pathways with potential prognostic value for CRC.MethodsAn...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Surgery |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsurg.2022.913967/full |
| _version_ | 1818515369048932352 |
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| author | Feng Zhuang Xuesong Bai Yang Shi Le Chang Wanchao Ai Juan Du Wei Liu Humin Liu Xukun Zhou Zhong Wang Tao Hong |
| author_facet | Feng Zhuang Xuesong Bai Yang Shi Le Chang Wanchao Ai Juan Du Wei Liu Humin Liu Xukun Zhou Zhong Wang Tao Hong |
| author_sort | Feng Zhuang |
| collection | DOAJ |
| description | BackgroundColorectal cancer (CRC) is one of the most common malignant tumors with recurrence and metastasis after surgical resection. This study aimed to identify the physiological changes after surgery and explore metabolites and metabolic pathways with potential prognostic value for CRC.MethodsAn ultra-high-performance liquid chromatography Q-exactive mass spectrometry was used to profile serum metabolites from 67 CRC patients and 50 healthy volunteers. Principal component analysis (PCA) and orthogonal projections to latent structures-discriminant analysis were used to distinguish the internal characteristics of data in different groups. Multivariate statistics were compiled to screen the significant metabolites and metabolic pathways.ResultA total of 180 metabolites were detected. Under the conditions of variable importance in projection >1 and p-value <0.05, 46 differentially expressed metabolites were screened for further pathway enrichment analysis. Based on the Kyoto Encyclopedia of Genes and Genomes database and Small Molecule Pathway Database, three metabolic pathways—arginine and proline metabolism, ascorbate and aldarate metabolism, and phenylalanine metabolism—were significantly altered after surgical resection and identified as associated with the removal of CRC. Notably, gamma-linolenic acid was upregulated in the CRC preoperative patients compared with those in healthy volunteers but returned to healthy levels after surgery.ConclusionThrough serum-based metabolomics, our study demonstrated the differential metabolic characteristics in CRC patients after surgery compared with those before surgery. Our results suggested that metabonomic analysis may be a powerful method for exploring physiological alterations in CRC patients after surgery as well as a useful tool for identifying candidate biomarkers and monitoring disease recurrence. |
| first_indexed | 2024-12-11T00:28:01Z |
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| institution | Directory Open Access Journal |
| issn | 2296-875X |
| language | English |
| last_indexed | 2024-12-11T00:28:01Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Surgery |
| spelling | doaj.art-8502d176014748f2a3ebda32db7974392022-12-22T01:27:29ZengFrontiers Media S.A.Frontiers in Surgery2296-875X2022-08-01910.3389/fsurg.2022.913967913967Metabolomic profiling identifies biomarkers and metabolic impacts of surgery for colorectal cancerFeng Zhuang0Xuesong Bai1Yang Shi2Le Chang3Wanchao Ai4Juan Du5Wei Liu6Humin Liu7Xukun Zhou8Zhong Wang9Tao Hong10General Surgery Department, Hospital of Xinjiang Production and Construction Corps, Urumchi, ChinaGeneral Surgery Department, Peking Union Medical College Hospital, China Academy of Medical Science & Peking Union Medical College, Beijing, ChinaGeneral Surgery Department, Hospital of Xinjiang Production and Construction Corps, Urumchi, ChinaGeneral Surgery Department, Hospital of Xinjiang Production and Construction Corps, Urumchi, ChinaGeneral Surgery Department, Hospital of Xinjiang Production and Construction Corps, Urumchi, ChinaGeneral Surgery Department, Hospital of Xinjiang Production and Construction Corps, Urumchi, ChinaGeneral Surgery Department, Peking Union Medical College Hospital, China Academy of Medical Science & Peking Union Medical College, Beijing, ChinaGeneral Surgery Department, Hospital of Xinjiang Production and Construction Corps, Urumchi, ChinaGeneral Surgery Department, Hospital of Xinjiang Production and Construction Corps, Urumchi, ChinaGeneral Surgery Department, Hospital of Xinjiang Production and Construction Corps, Urumchi, ChinaGeneral Surgery Department, Peking Union Medical College Hospital, China Academy of Medical Science & Peking Union Medical College, Beijing, ChinaBackgroundColorectal cancer (CRC) is one of the most common malignant tumors with recurrence and metastasis after surgical resection. This study aimed to identify the physiological changes after surgery and explore metabolites and metabolic pathways with potential prognostic value for CRC.MethodsAn ultra-high-performance liquid chromatography Q-exactive mass spectrometry was used to profile serum metabolites from 67 CRC patients and 50 healthy volunteers. Principal component analysis (PCA) and orthogonal projections to latent structures-discriminant analysis were used to distinguish the internal characteristics of data in different groups. Multivariate statistics were compiled to screen the significant metabolites and metabolic pathways.ResultA total of 180 metabolites were detected. Under the conditions of variable importance in projection >1 and p-value <0.05, 46 differentially expressed metabolites were screened for further pathway enrichment analysis. Based on the Kyoto Encyclopedia of Genes and Genomes database and Small Molecule Pathway Database, three metabolic pathways—arginine and proline metabolism, ascorbate and aldarate metabolism, and phenylalanine metabolism—were significantly altered after surgical resection and identified as associated with the removal of CRC. Notably, gamma-linolenic acid was upregulated in the CRC preoperative patients compared with those in healthy volunteers but returned to healthy levels after surgery.ConclusionThrough serum-based metabolomics, our study demonstrated the differential metabolic characteristics in CRC patients after surgery compared with those before surgery. Our results suggested that metabonomic analysis may be a powerful method for exploring physiological alterations in CRC patients after surgery as well as a useful tool for identifying candidate biomarkers and monitoring disease recurrence.https://www.frontiersin.org/articles/10.3389/fsurg.2022.913967/fullcolorectal cancermetabolomic profilingtumorectomygamma-linolenic acid (GLA)prolineascorbate |
| spellingShingle | Feng Zhuang Xuesong Bai Yang Shi Le Chang Wanchao Ai Juan Du Wei Liu Humin Liu Xukun Zhou Zhong Wang Tao Hong Metabolomic profiling identifies biomarkers and metabolic impacts of surgery for colorectal cancer Frontiers in Surgery colorectal cancer metabolomic profiling tumorectomy gamma-linolenic acid (GLA) proline ascorbate |
| title | Metabolomic profiling identifies biomarkers and metabolic impacts of surgery for colorectal cancer |
| title_full | Metabolomic profiling identifies biomarkers and metabolic impacts of surgery for colorectal cancer |
| title_fullStr | Metabolomic profiling identifies biomarkers and metabolic impacts of surgery for colorectal cancer |
| title_full_unstemmed | Metabolomic profiling identifies biomarkers and metabolic impacts of surgery for colorectal cancer |
| title_short | Metabolomic profiling identifies biomarkers and metabolic impacts of surgery for colorectal cancer |
| title_sort | metabolomic profiling identifies biomarkers and metabolic impacts of surgery for colorectal cancer |
| topic | colorectal cancer metabolomic profiling tumorectomy gamma-linolenic acid (GLA) proline ascorbate |
| url | https://www.frontiersin.org/articles/10.3389/fsurg.2022.913967/full |
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