Prevalence of Enterotoxin Genes and spa Genotypes of Methicillin-resistant Staphylococcus aureus from a Tertiary Care Hospital in China
Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen that causes a variety of infections. MRSA has evolved resistance to multiple antibiotics. Genetic background and virulence differs in different geographic regions. The present study was aimed to investi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2015-05-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5990/12090_CE[Ra]_F(P)_PF1(VSUAK)_PFA(AK)_PF2(AGAK).pdf |
| _version_ | 1811245976788140032 |
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| author | Yanmeng Li Ruike Zhao Xianfeng Zhang Qingzhen Han Xuefeng Qian Guohao Gu Jinfang Shi Jie Xu |
| author_facet | Yanmeng Li Ruike Zhao Xianfeng Zhang Qingzhen Han Xuefeng Qian Guohao Gu Jinfang Shi Jie Xu |
| author_sort | Yanmeng Li |
| collection | DOAJ |
| description | Objectives: Methicillin-resistant Staphylococcus aureus (MRSA)
is a major nosocomial pathogen that causes a variety of
infections. MRSA has evolved resistance to multiple antibiotics.
Genetic background and virulence differs in different geographic
regions. The present study was aimed to investigate the
prevalence of enterotoxin genes and spa genotypes of hospitalassociated methicillin-resistant Staphylococcus aureus (HAMRSA) isolated from a tertiary care hospital of Jiangsu province,
China.
Materials and Methods: HA-MRSA isolates from August 2013
to April 2014 at a tertiary care hospital of China were collected.
We investigated antimicrobial pattern, spa types, SCCmec
types and the presence of 14 virulence genes.
Results: Eighty HA-MRSA isolates were collected. Results from
SCCmec typing revealed that 73.8% were type II; 13.8% were
type III; 12.5% were type V. There were 19 different spa types.
Spa type t2460 was the most common (35.0%), followed by
t002 (11.3%). CC5 was the predominant MLST CCs type (50%).
The most frequent toxin genes were sea, seb, sed, sel, sen and
seo (100.0%). None of the investigated isolates carried the sec
or tst.
Conclusion: Genotypic and virulence evaluation of the isolated
HA-MRSA revealed that the isolates with CC5 and SCCmec
II were the predominant type and highly homological. The
virulence profiles mainly existed in the genes of sea, seb, sed,
sel, sen, seo and ser. The prevalence of t2460 was an outbreak
and the predominant spa type. |
| first_indexed | 2024-04-12T14:46:44Z |
| format | Article |
| id | doaj.art-8504fb1f57cd4969b56306cf6e530447 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-12T14:46:44Z |
| publishDate | 2015-05-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-8504fb1f57cd4969b56306cf6e5304472022-12-22T03:28:37ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-05-0195DC11DC1410.7860/JCDR/2015/12090.5990Prevalence of Enterotoxin Genes and spa Genotypes of Methicillin-resistant Staphylococcus aureus from a Tertiary Care Hospital in ChinaYanmeng Li0Ruike Zhao1Xianfeng Zhang2Qingzhen Han3Xuefeng Qian4Guohao Gu5Jinfang Shi6Jie Xu7Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen that causes a variety of infections. MRSA has evolved resistance to multiple antibiotics. Genetic background and virulence differs in different geographic regions. The present study was aimed to investigate the prevalence of enterotoxin genes and spa genotypes of hospitalassociated methicillin-resistant Staphylococcus aureus (HAMRSA) isolated from a tertiary care hospital of Jiangsu province, China. Materials and Methods: HA-MRSA isolates from August 2013 to April 2014 at a tertiary care hospital of China were collected. We investigated antimicrobial pattern, spa types, SCCmec types and the presence of 14 virulence genes. Results: Eighty HA-MRSA isolates were collected. Results from SCCmec typing revealed that 73.8% were type II; 13.8% were type III; 12.5% were type V. There were 19 different spa types. Spa type t2460 was the most common (35.0%), followed by t002 (11.3%). CC5 was the predominant MLST CCs type (50%). The most frequent toxin genes were sea, seb, sed, sel, sen and seo (100.0%). None of the investigated isolates carried the sec or tst. Conclusion: Genotypic and virulence evaluation of the isolated HA-MRSA revealed that the isolates with CC5 and SCCmec II were the predominant type and highly homological. The virulence profiles mainly existed in the genes of sea, seb, sed, sel, sen, seo and ser. The prevalence of t2460 was an outbreak and the predominant spa type.https://jcdr.net/articles/PDF/5990/12090_CE[Ra]_F(P)_PF1(VSUAK)_PFA(AK)_PF2(AGAK).pdfseasebsedselsenseosert2460 |
| spellingShingle | Yanmeng Li Ruike Zhao Xianfeng Zhang Qingzhen Han Xuefeng Qian Guohao Gu Jinfang Shi Jie Xu Prevalence of Enterotoxin Genes and spa Genotypes of Methicillin-resistant Staphylococcus aureus from a Tertiary Care Hospital in China Journal of Clinical and Diagnostic Research sea seb sed sel sen seo ser t2460 |
| title | Prevalence of Enterotoxin Genes and spa Genotypes of Methicillin-resistant Staphylococcus aureus from a Tertiary Care Hospital in China |
| title_full | Prevalence of Enterotoxin Genes and spa Genotypes of Methicillin-resistant Staphylococcus aureus from a Tertiary Care Hospital in China |
| title_fullStr | Prevalence of Enterotoxin Genes and spa Genotypes of Methicillin-resistant Staphylococcus aureus from a Tertiary Care Hospital in China |
| title_full_unstemmed | Prevalence of Enterotoxin Genes and spa Genotypes of Methicillin-resistant Staphylococcus aureus from a Tertiary Care Hospital in China |
| title_short | Prevalence of Enterotoxin Genes and spa Genotypes of Methicillin-resistant Staphylococcus aureus from a Tertiary Care Hospital in China |
| title_sort | prevalence of enterotoxin genes and spa genotypes of methicillin resistant staphylococcus aureus from a tertiary care hospital in china |
| topic | sea seb sed sel sen seo ser t2460 |
| url | https://jcdr.net/articles/PDF/5990/12090_CE[Ra]_F(P)_PF1(VSUAK)_PFA(AK)_PF2(AGAK).pdf |
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