Baicalein blocked cervical carcinoma cell proliferation by targeting CCND1 via Wnt/β-catenin signaling pathway
The purpose of this study was to investigate the inhibitory effect of baicalein on the proliferation of cervical carcinoma cells and stimulate cervical carcinoma cells with baicalein. MTT method was used to observe cell proliferation. Flow cytometry was used to observe cell cycle, and gene technolog...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1636055 |
| _version_ | 1811291876686299136 |
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| author | Xiulian Xia Jiyi Xia Hai Yang Yan Li Shengyue Liu Yong Cao Li Tang Xiaolan Yu |
| author_facet | Xiulian Xia Jiyi Xia Hai Yang Yan Li Shengyue Liu Yong Cao Li Tang Xiaolan Yu |
| author_sort | Xiulian Xia |
| collection | DOAJ |
| description | The purpose of this study was to investigate the inhibitory effect of baicalein on the proliferation of cervical carcinoma cells and stimulate cervical carcinoma cells with baicalein. MTT method was used to observe cell proliferation. Flow cytometry was used to observe cell cycle, and gene technology was used to observe the expression of corresponding genes at the level of gene and protein. β-catenin activity was assessed using Western blot and ChIP. Baicalein suppressed cervical carcinoma cell HeLa proliferation by enhancing the activity of caspase-3. Baicalein blocked cell cycle at G0/G1 stage by inhibiting the expression of some genes. At the same time, it can prevent the nuclear translocation of β-catenin and inhibit the activity of Wnt. When the Wnt signaling pathway is increased, the proliferation of HeLa cells is inhibited, and apoptosis is promoted in this way. In conclusion, it indicated that baicalein inhibits cervical carcinoma progression by targeting CCND1 via Wnt/β-catenin signaling pathway. |
| first_indexed | 2024-04-13T04:35:57Z |
| format | Article |
| id | doaj.art-850836d2d5554afb9fcb10381410903c |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T04:35:57Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-850836d2d5554afb9fcb10381410903c2022-12-22T03:02:09ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014712729273610.1080/21691401.2019.1636055Baicalein blocked cervical carcinoma cell proliferation by targeting CCND1 via Wnt/β-catenin signaling pathwayXiulian Xia0Jiyi Xia1Hai Yang2Yan Li3Shengyue Liu4Yong Cao5Li Tang6Xiaolan Yu7Department of Obstetrics and Gynecology, The Affiliated TCM Hospital of South West Medical University, Luzhou, ChinaSchool of Medical Information and Engineering, Southwest Medical University, Luzhou, ChinaDepartment of Obstetrics and Gynecology, The Affiliated TCM Hospital of South West Medical University, Luzhou, ChinaMedicine Experimental Center, The Affiliated Hospital of Southwest Medical University, Luzhou, ChinaDepartment of Obstetrics and Gynecology, The Affiliated TCM Hospital of South West Medical University, Luzhou, ChinaMedicine Experimental Center, The Affiliated Hospital of Southwest Medical University, Luzhou, ChinaMedicine Experimental Center, The Affiliated Hospital of Southwest Medical University, Luzhou, ChinaDepartment of Obstetrics and Gynecology, The Affiliated TCM Hospital of South West Medical University, Luzhou, ChinaThe purpose of this study was to investigate the inhibitory effect of baicalein on the proliferation of cervical carcinoma cells and stimulate cervical carcinoma cells with baicalein. MTT method was used to observe cell proliferation. Flow cytometry was used to observe cell cycle, and gene technology was used to observe the expression of corresponding genes at the level of gene and protein. β-catenin activity was assessed using Western blot and ChIP. Baicalein suppressed cervical carcinoma cell HeLa proliferation by enhancing the activity of caspase-3. Baicalein blocked cell cycle at G0/G1 stage by inhibiting the expression of some genes. At the same time, it can prevent the nuclear translocation of β-catenin and inhibit the activity of Wnt. When the Wnt signaling pathway is increased, the proliferation of HeLa cells is inhibited, and apoptosis is promoted in this way. In conclusion, it indicated that baicalein inhibits cervical carcinoma progression by targeting CCND1 via Wnt/β-catenin signaling pathway.https://www.tandfonline.com/doi/10.1080/21691401.2019.1636055Baicaleincervical carcinomaCCND1Wnt/β-catenin pathwayproliferation |
| spellingShingle | Xiulian Xia Jiyi Xia Hai Yang Yan Li Shengyue Liu Yong Cao Li Tang Xiaolan Yu Baicalein blocked cervical carcinoma cell proliferation by targeting CCND1 via Wnt/β-catenin signaling pathway Artificial Cells, Nanomedicine, and Biotechnology Baicalein cervical carcinoma CCND1 Wnt/β-catenin pathway proliferation |
| title | Baicalein blocked cervical carcinoma cell proliferation by targeting CCND1 via Wnt/β-catenin signaling pathway |
| title_full | Baicalein blocked cervical carcinoma cell proliferation by targeting CCND1 via Wnt/β-catenin signaling pathway |
| title_fullStr | Baicalein blocked cervical carcinoma cell proliferation by targeting CCND1 via Wnt/β-catenin signaling pathway |
| title_full_unstemmed | Baicalein blocked cervical carcinoma cell proliferation by targeting CCND1 via Wnt/β-catenin signaling pathway |
| title_short | Baicalein blocked cervical carcinoma cell proliferation by targeting CCND1 via Wnt/β-catenin signaling pathway |
| title_sort | baicalein blocked cervical carcinoma cell proliferation by targeting ccnd1 via wnt β catenin signaling pathway |
| topic | Baicalein cervical carcinoma CCND1 Wnt/β-catenin pathway proliferation |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1636055 |
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