Additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regeneration
The study focused on the effects of a triply periodic minimal surface (TPMS) scaffolds, varying in porosity, on the repair of mandibular defects in New Zealand white rabbits. Four TPMS configurations (40%, 50%, 60%, and 70% porosity) were fabricated with β-tricalcium phosphate bioceramic via additiv...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2024-04-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314241244997 |
| _version_ | 1797212990247796736 |
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| author | Hong Zhu Jinsi Wang Shengfa Wang Yue Yang Meiyi Chen Qifei Luan Xiaochuan Liu Ziheng Lin Jiaqi Hu Kenny Man Jingying Zhang |
| author_facet | Hong Zhu Jinsi Wang Shengfa Wang Yue Yang Meiyi Chen Qifei Luan Xiaochuan Liu Ziheng Lin Jiaqi Hu Kenny Man Jingying Zhang |
| author_sort | Hong Zhu |
| collection | DOAJ |
| description | The study focused on the effects of a triply periodic minimal surface (TPMS) scaffolds, varying in porosity, on the repair of mandibular defects in New Zealand white rabbits. Four TPMS configurations (40%, 50%, 60%, and 70% porosity) were fabricated with β-tricalcium phosphate bioceramic via additive manufacturing. Scaffold properties were assessed through scanning electron microscopy and mechanical testing. For proliferation and adhesion assays, mouse bone marrow stem cells (BMSCs) were cultured on these scaffolds. In vivo, the scaffolds were implanted into rabbit mandibular defects for 2 months. Histological staining evaluated osteogenic potential. Moreover, RNA-sequencing analysis and RT-qPCR revealed the significant involvement of angiogenesis-related factors and Hippo signaling pathway in influencing BMSCs behavior. Notably, the 70% porosity TPMS scaffold exhibited optimal compressive strength, superior cell proliferation, adhesion, and significantly enhanced osteogenesis and angiogenesis. These findings underscore the substantial potential of 70% porosity TPMS scaffolds in effectively promoting bone regeneration within mandibular defects. |
| first_indexed | 2024-04-24T10:51:09Z |
| format | Article |
| id | doaj.art-850d10b0c1a6496db4cf2b8c58783b01 |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-04-24T10:51:09Z |
| publishDate | 2024-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-850d10b0c1a6496db4cf2b8c58783b012024-04-12T12:03:24ZengSAGE PublishingJournal of Tissue Engineering2041-73142024-04-011510.1177/20417314241244997Additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regenerationHong Zhu0Jinsi Wang1Shengfa Wang2Yue Yang3Meiyi Chen4Qifei Luan5Xiaochuan Liu6Ziheng Lin7Jiaqi Hu8Kenny Man9Jingying Zhang10The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. ChinaThe First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. ChinaDalian University of Technology, Dalian, P.R. ChinaThe First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. ChinaThe First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. ChinaThe First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. ChinaThe First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. ChinaThe First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. ChinaThe First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. ChinaRegenerative Medicine Center Urecht, Utrecht, The NetherlandsThe First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. ChinaThe study focused on the effects of a triply periodic minimal surface (TPMS) scaffolds, varying in porosity, on the repair of mandibular defects in New Zealand white rabbits. Four TPMS configurations (40%, 50%, 60%, and 70% porosity) were fabricated with β-tricalcium phosphate bioceramic via additive manufacturing. Scaffold properties were assessed through scanning electron microscopy and mechanical testing. For proliferation and adhesion assays, mouse bone marrow stem cells (BMSCs) were cultured on these scaffolds. In vivo, the scaffolds were implanted into rabbit mandibular defects for 2 months. Histological staining evaluated osteogenic potential. Moreover, RNA-sequencing analysis and RT-qPCR revealed the significant involvement of angiogenesis-related factors and Hippo signaling pathway in influencing BMSCs behavior. Notably, the 70% porosity TPMS scaffold exhibited optimal compressive strength, superior cell proliferation, adhesion, and significantly enhanced osteogenesis and angiogenesis. These findings underscore the substantial potential of 70% porosity TPMS scaffolds in effectively promoting bone regeneration within mandibular defects.https://doi.org/10.1177/20417314241244997 |
| spellingShingle | Hong Zhu Jinsi Wang Shengfa Wang Yue Yang Meiyi Chen Qifei Luan Xiaochuan Liu Ziheng Lin Jiaqi Hu Kenny Man Jingying Zhang Additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regeneration Journal of Tissue Engineering |
| title | Additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regeneration |
| title_full | Additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regeneration |
| title_fullStr | Additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regeneration |
| title_full_unstemmed | Additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regeneration |
| title_short | Additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regeneration |
| title_sort | additively manufactured bioceramic scaffolds based on triply periodic minimal surfaces for bone regeneration |
| url | https://doi.org/10.1177/20417314241244997 |
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