The Interaction between Enterobacteriaceae and Calcium Oxalate Deposits.
The role of calcium oxalate crystals and deposits in UTI pathogenesis has not been established. The objectives of this study were to identify bacteria present in pediatric urolithiasis and, using in vitro and in vivo models, to determine the relevance of calcium oxalate deposits during experimental...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4598009?pdf=render |
| _version_ | 1818319016778792960 |
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| author | Evan Barr-Beare Vijay Saxena Evann E Hilt Krystal Thomas-White Megan Schober Birong Li Brian Becknell David S Hains Alan J Wolfe Andrew L Schwaderer |
| author_facet | Evan Barr-Beare Vijay Saxena Evann E Hilt Krystal Thomas-White Megan Schober Birong Li Brian Becknell David S Hains Alan J Wolfe Andrew L Schwaderer |
| author_sort | Evan Barr-Beare |
| collection | DOAJ |
| description | The role of calcium oxalate crystals and deposits in UTI pathogenesis has not been established. The objectives of this study were to identify bacteria present in pediatric urolithiasis and, using in vitro and in vivo models, to determine the relevance of calcium oxalate deposits during experimental pyelonephritis.Pediatric kidney stones and urine were collected and both cultured and sequenced for bacteria. Bacterial adhesion to calcium oxalate was compared. Murine kidney calcium oxalate deposits were induced by intraperitoneal glyoxalate injection and kidneys were transurethrally inoculated with uropathogenic Escherichia coli to induce pyelonephritis.E. coli of the family Enterobacteriaceae was identified in patients by calcium oxalate stone culture. Additionally Enterobacteriaceae DNA was sequenced from multiple calcium oxalate kidney stones. E. coli selectively aggregated on and around calcium oxalate monohydrate crystals. Mice inoculated with glyoxalate and uropathogenic E. coli had higher bacterial burdens, increased kidney calcium oxalate deposits and an increased kidney innate immune response compared to mice with only calcium oxalate deposits or only pyelonephritis.In a murine model, the presence of calcium oxalate deposits increases pyelonephritis risk, likely due to preferential aggregation of bacteria on and around calcium oxalate crystals. When both calcium oxalate deposits and uropathogenic bacteria were present, calcium oxalate deposit number increased along with renal gene transcription of inner stone core matrix proteins increased. Therefore renal calcium oxalate deposits may be a modifiable risk factor for infections of the kidney and urinary tract. Furthermore, bacteria may be present in calcium oxalate deposits and potentially contribute to calcium oxalate renal disease. |
| first_indexed | 2024-12-13T10:02:25Z |
| format | Article |
| id | doaj.art-850da49c32444a1fbf924734154ecf58 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T10:02:25Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-850da49c32444a1fbf924734154ecf582022-12-21T23:51:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011010e013957510.1371/journal.pone.0139575The Interaction between Enterobacteriaceae and Calcium Oxalate Deposits.Evan Barr-BeareVijay SaxenaEvann E HiltKrystal Thomas-WhiteMegan SchoberBirong LiBrian BecknellDavid S HainsAlan J WolfeAndrew L SchwadererThe role of calcium oxalate crystals and deposits in UTI pathogenesis has not been established. The objectives of this study were to identify bacteria present in pediatric urolithiasis and, using in vitro and in vivo models, to determine the relevance of calcium oxalate deposits during experimental pyelonephritis.Pediatric kidney stones and urine were collected and both cultured and sequenced for bacteria. Bacterial adhesion to calcium oxalate was compared. Murine kidney calcium oxalate deposits were induced by intraperitoneal glyoxalate injection and kidneys were transurethrally inoculated with uropathogenic Escherichia coli to induce pyelonephritis.E. coli of the family Enterobacteriaceae was identified in patients by calcium oxalate stone culture. Additionally Enterobacteriaceae DNA was sequenced from multiple calcium oxalate kidney stones. E. coli selectively aggregated on and around calcium oxalate monohydrate crystals. Mice inoculated with glyoxalate and uropathogenic E. coli had higher bacterial burdens, increased kidney calcium oxalate deposits and an increased kidney innate immune response compared to mice with only calcium oxalate deposits or only pyelonephritis.In a murine model, the presence of calcium oxalate deposits increases pyelonephritis risk, likely due to preferential aggregation of bacteria on and around calcium oxalate crystals. When both calcium oxalate deposits and uropathogenic bacteria were present, calcium oxalate deposit number increased along with renal gene transcription of inner stone core matrix proteins increased. Therefore renal calcium oxalate deposits may be a modifiable risk factor for infections of the kidney and urinary tract. Furthermore, bacteria may be present in calcium oxalate deposits and potentially contribute to calcium oxalate renal disease.http://europepmc.org/articles/PMC4598009?pdf=render |
| spellingShingle | Evan Barr-Beare Vijay Saxena Evann E Hilt Krystal Thomas-White Megan Schober Birong Li Brian Becknell David S Hains Alan J Wolfe Andrew L Schwaderer The Interaction between Enterobacteriaceae and Calcium Oxalate Deposits. PLoS ONE |
| title | The Interaction between Enterobacteriaceae and Calcium Oxalate Deposits. |
| title_full | The Interaction between Enterobacteriaceae and Calcium Oxalate Deposits. |
| title_fullStr | The Interaction between Enterobacteriaceae and Calcium Oxalate Deposits. |
| title_full_unstemmed | The Interaction between Enterobacteriaceae and Calcium Oxalate Deposits. |
| title_short | The Interaction between Enterobacteriaceae and Calcium Oxalate Deposits. |
| title_sort | interaction between enterobacteriaceae and calcium oxalate deposits |
| url | http://europepmc.org/articles/PMC4598009?pdf=render |
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