Alternative splicing produces two transcripts encoding female-biased pheromone subfamily receptors in the navel orangeworm, Amyelois transitella
Insect odorant receptors are key sensors of environmental odors and members of the lepidopteran pheromone receptor subfamily are thought to play important roles in mate finding by recognizing sex pheromones. Much research has been done to identify putative pheromone receptors in lepidopteran males,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-10-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fevo.2015.00115/full |
| _version_ | 1828485017085411328 |
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| author | Stephen F Garczynski Walter Soares Leal |
| author_facet | Stephen F Garczynski Walter Soares Leal |
| author_sort | Stephen F Garczynski |
| collection | DOAJ |
| description | Insect odorant receptors are key sensors of environmental odors and members of the lepidopteran pheromone receptor subfamily are thought to play important roles in mate finding by recognizing sex pheromones. Much research has been done to identify putative pheromone receptors in lepidopteran males, but little attention has been given to female counterparts. In this study, degenerate oligonucleotide primers designed against a conserved amino acid region in the C-terminus of lepidopteran pheromone receptors were used in 3’ RACE reactions to identify candidate pheromone receptors expressed in the antennae of female navel orangeworm. Two near full-length transcripts of 1469 nt and 1302 nt encoding the complete open reading frames for proteins of 446 and 425 amino acids, respectively, were identified. Based on BLAST homology and phylogenetic analyses, the putative proteins encoded by these transcripts are members of the lepidopteran pheromone receptor subfamily. Characterization of these transcripts indicates that they are alternatively spliced products of a single gene. Tissue expression studies indicate that the transcripts are female-biased with detection mainly in female antennae. To the best of our knowledge, these transcripts represent the first detection of alternatively spliced female-biased members of the lepidopteran pheromone receptor subfamily. |
| first_indexed | 2024-12-11T09:05:34Z |
| format | Article |
| id | doaj.art-850eb490fcf04bba9912ace94cee39b3 |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-12-11T09:05:34Z |
| publishDate | 2015-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-850eb490fcf04bba9912ace94cee39b32022-12-22T01:13:38ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2015-10-01310.3389/fevo.2015.00115160338Alternative splicing produces two transcripts encoding female-biased pheromone subfamily receptors in the navel orangeworm, Amyelois transitellaStephen F Garczynski0Walter Soares Leal1United States Department of Agriculture - Agricultural Research ServiceUniversity of California DavisInsect odorant receptors are key sensors of environmental odors and members of the lepidopteran pheromone receptor subfamily are thought to play important roles in mate finding by recognizing sex pheromones. Much research has been done to identify putative pheromone receptors in lepidopteran males, but little attention has been given to female counterparts. In this study, degenerate oligonucleotide primers designed against a conserved amino acid region in the C-terminus of lepidopteran pheromone receptors were used in 3’ RACE reactions to identify candidate pheromone receptors expressed in the antennae of female navel orangeworm. Two near full-length transcripts of 1469 nt and 1302 nt encoding the complete open reading frames for proteins of 446 and 425 amino acids, respectively, were identified. Based on BLAST homology and phylogenetic analyses, the putative proteins encoded by these transcripts are members of the lepidopteran pheromone receptor subfamily. Characterization of these transcripts indicates that they are alternatively spliced products of a single gene. Tissue expression studies indicate that the transcripts are female-biased with detection mainly in female antennae. To the best of our knowledge, these transcripts represent the first detection of alternatively spliced female-biased members of the lepidopteran pheromone receptor subfamily.http://journal.frontiersin.org/Journal/10.3389/fevo.2015.00115/fullAlternative Splicingodorant receptorNavel orangewormpheromone receptor subfamily3’ RACE |
| spellingShingle | Stephen F Garczynski Walter Soares Leal Alternative splicing produces two transcripts encoding female-biased pheromone subfamily receptors in the navel orangeworm, Amyelois transitella Frontiers in Ecology and Evolution Alternative Splicing odorant receptor Navel orangeworm pheromone receptor subfamily 3’ RACE |
| title | Alternative splicing produces two transcripts encoding female-biased pheromone subfamily receptors in the navel orangeworm, Amyelois transitella |
| title_full | Alternative splicing produces two transcripts encoding female-biased pheromone subfamily receptors in the navel orangeworm, Amyelois transitella |
| title_fullStr | Alternative splicing produces two transcripts encoding female-biased pheromone subfamily receptors in the navel orangeworm, Amyelois transitella |
| title_full_unstemmed | Alternative splicing produces two transcripts encoding female-biased pheromone subfamily receptors in the navel orangeworm, Amyelois transitella |
| title_short | Alternative splicing produces two transcripts encoding female-biased pheromone subfamily receptors in the navel orangeworm, Amyelois transitella |
| title_sort | alternative splicing produces two transcripts encoding female biased pheromone subfamily receptors in the navel orangeworm amyelois transitella |
| topic | Alternative Splicing odorant receptor Navel orangeworm pheromone receptor subfamily 3’ RACE |
| url | http://journal.frontiersin.org/Journal/10.3389/fevo.2015.00115/full |
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