Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2
DNA testing in cases of disputed paternity is a routine analysis carried out in genetic laboratories. The purpose of the test is to demonstrate similarities and differences in analyzed genetic markers between the alleged father, mother, and a child. The existence of differences in the examined loci...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/1/62 |
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| author | Andrzej Doniec Wojciech Łuczak Maria Wróbel Miłosz Januła Andrzej Ossowski Paweł Grzmil Tomasz Kupiec |
| author_facet | Andrzej Doniec Wojciech Łuczak Maria Wróbel Miłosz Januła Andrzej Ossowski Paweł Grzmil Tomasz Kupiec |
| author_sort | Andrzej Doniec |
| collection | DOAJ |
| description | DNA testing in cases of disputed paternity is a routine analysis carried out in genetic laboratories. The purpose of the test is to demonstrate similarities and differences in analyzed genetic markers between the alleged father, mother, and a child. The existence of differences in the examined loci between the child and the presumed father may indicate the exclusion of biological parenthood. However, another reason for such differences is genetic mutations, including chromosome aberrations and genome mutations. The presented results relate to genetic analyses carried out on three persons for the purposes of disputed paternity testing. A deviation from inheritance based on Mendel’s Law was found in 7 out of 53 STR-type loci examined. All polymorphic loci that ruled out the paternity of the alleged father were located on chromosome 2. Additional analysis of 32 insertion–deletion markers (DIPplex, Qiagen) and sequencing of 94 polymorphic positions of the single nucleotide polymorphism (SNP) type (Illumina, ForenSeq) did not exclude the defendant’s biological paternity. A sequence analysis of STR alleles and their flanking regions confirmed the hypothesis that the alleles on chromosome 2 of the child may originate only from the mother. The results of the tests did not allow exclusion of the paternity of the alleged father, but are an example of uniparental maternal disomy, which is briefly described in the literature. |
| first_indexed | 2024-03-10T13:30:05Z |
| format | Article |
| id | doaj.art-850eee7090d8453cafefcb91f5f987ba |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T13:30:05Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-850eee7090d8453cafefcb91f5f987ba2023-11-21T08:09:12ZengMDPI AGGenes2073-44252021-01-011216210.3390/genes12010062Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2Andrzej Doniec0Wojciech Łuczak1Maria Wróbel2Miłosz Januła3Andrzej Ossowski4Paweł Grzmil5Tomasz Kupiec6Department of Genetics and Evolutionism, Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, PolandLaboratory of Molecular Diagnostics Genmed, ul. Św. Marcin 49, 61-806 Poznań, PolandForensic Genetics Section, Institute of Forensic Research, Westerplatte 9, 31-033 Kraków, PolandForensic Genetics Section, Institute of Forensic Research, Westerplatte 9, 31-033 Kraków, PolandDepartment of Forensic Medicine, Pomeranian Medical University of Szczecin, Powstańców Wielkopolskich 72, 70-111 Szczecin, PolandDepartment of Genetics and Evolutionism, Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, PolandForensic Genetics Section, Institute of Forensic Research, Westerplatte 9, 31-033 Kraków, PolandDNA testing in cases of disputed paternity is a routine analysis carried out in genetic laboratories. The purpose of the test is to demonstrate similarities and differences in analyzed genetic markers between the alleged father, mother, and a child. The existence of differences in the examined loci between the child and the presumed father may indicate the exclusion of biological parenthood. However, another reason for such differences is genetic mutations, including chromosome aberrations and genome mutations. The presented results relate to genetic analyses carried out on three persons for the purposes of disputed paternity testing. A deviation from inheritance based on Mendel’s Law was found in 7 out of 53 STR-type loci examined. All polymorphic loci that ruled out the paternity of the alleged father were located on chromosome 2. Additional analysis of 32 insertion–deletion markers (DIPplex, Qiagen) and sequencing of 94 polymorphic positions of the single nucleotide polymorphism (SNP) type (Illumina, ForenSeq) did not exclude the defendant’s biological paternity. A sequence analysis of STR alleles and their flanking regions confirmed the hypothesis that the alleles on chromosome 2 of the child may originate only from the mother. The results of the tests did not allow exclusion of the paternity of the alleged father, but are an example of uniparental maternal disomy, which is briefly described in the literature.https://www.mdpi.com/2073-4425/12/1/62uniparental disomypaternity testingtrisomy rescueshort tandem repeat profiling |
| spellingShingle | Andrzej Doniec Wojciech Łuczak Maria Wróbel Miłosz Januła Andrzej Ossowski Paweł Grzmil Tomasz Kupiec Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2 Genes uniparental disomy paternity testing trisomy rescue short tandem repeat profiling |
| title | Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2 |
| title_full | Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2 |
| title_fullStr | Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2 |
| title_full_unstemmed | Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2 |
| title_short | Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2 |
| title_sort | confirmation of paternity despite three genetic incompatibilities at chromosome 2 |
| topic | uniparental disomy paternity testing trisomy rescue short tandem repeat profiling |
| url | https://www.mdpi.com/2073-4425/12/1/62 |
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